Device and method for applying a continuous electric field
An electric field, a technology for manufacturing devices, which is applied in the field of devices for applying electric fields, can solve the problems of analyte loss, incomplete reduction of electric field distortion, etc.
Inactive Publication Date: 2014-03-05
GENETIC MICRODEVICES
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However, in practice, this configuration does not completely reduce electric field distortion for two reasons
The first reason is that it is not possible to place an infinite number of independently addressable electrodes along the channel
The second reason is that because the electrodes have finite dimensions, the voltage in the space immediately adjacent to the electrodes is constant (at a value approximately equal to the voltage of the electrodes)
[0009] Another problem with conventional separation techniques, including known field shifting techniques, is that some analytes are lost and instead of traveling through the electrodes as intended, objects travel towards the electrodes in direct contact with the channel and are effectively removed from the separation process. remove
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[0101] It will be appreciated that the devices and methods according to the exemplary embodiments described herein have wide application and may be used in any device for applications requiring smooth electric fields, such as microfluidic devices or, for example, LOCs. Potential applications include electrophoresis-like techniques for separating objects or other separation techniques (eg centrifugal spectroscopy as described in EP1455949). Alternative applications include microfluidic devices for the separation of objects, for example in hydrophobic paper substrates, in "free-flow" electrophoretic devices, in plate coagulation or in hydrophilic regions provided in electrochromatography,
[0102] The exemplary embodiments described below will focus on electrophoretic applications for separating objects in channels, in particular electric field movement techniques of the type described in WO2006 / 070176, but it should be understood that this is not intended to be limiting.
[010...
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Abstract
A device comprises an electric field applying assembly adapted to generate an electric field having a discrete electric field profile; a conducting volume and an electrical interface region provided between the conducting volume and the electric field applying assembly such that the discrete electric field is applied to the material by the electric field applying assembly at a location spaced from the conducting volume,wherein the electrical interface region comprises at least an ionically conductive material arranged adjacent to and in contact with the conducting volume, such that the discrete electric field applied by the electric field applying assembly is smoothed by the electrical interface region so that the electric field profile established within the conducting volume is substantially continuous.
Description
technical field [0001] The present invention relates to a device and method for applying an electric field and a method of manufacturing the same. The invention is particularly suitable for smoothing the shape of an electric field applied to a microfluidic device or "lab-on-a-chip" type device (LOC). Background technique [0002] Microfluidic devices such as LOCs have a wide range of applications. An increasing number of these applications involve the sequencing and sorting of objects such as biomolecules and the sorting of cells. Typically, sequencing and sorting of biomolecules, and sorting of cells are performed using electrophoresis. Electrophoretic techniques are well known and are often used to separate objects (sometimes referred to as "analytes") according to their electrical and hydrodynamic properties. Other separation techniques include the use of centrifugal spectrometers as described in EP1455949. [0003] In conventional electrophoresis, a constant uniform ...
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IPC IPC(8): G01N27/447G01N27/453B01D57/02
CPCB01D57/02G01N27/44713G01N27/44756G01N27/447G01N27/44791Y10T29/49002G01N27/44773
Inventor D·赛德瑞斯A·伊勒斯R·杰克逊
Owner GENETIC MICRODEVICES