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Method for preparing and identifying recombinant bifidobacterium vaccine of taenia solium TSO45W-4B gene

A technology of TSO45W-4B and Taenia solium, which is applied in the field of preparation and identification of TSO45W-4B gene recombinant bifidobacteria vaccine of Taenia solium, and achieves the effects of convenient inoculation route, simple production and suitable for mass production

Inactive Publication Date: 2014-03-12
ZUNYI MEDICAL UNIVERSITY
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Problems solved by technology

In the field of parasites, there has not been any report on the recombinant Bb of Taenia solium. Therefore, this study intends to synthesize the TSO45W-4B gene of Taenia solium and clone it into the E. coli-Bifidobacterium shuttle expression vector pGEX- In 1λT, the recombinant plasmid pGEX-TSO45W-4B was constructed, and Bifidobacterium longum (B.longum) was transformed by electroporation, and the recombinant Bb (pGEX-TSO45W-4B) vaccine of Taenia solium was constructed to provide a vaccine for the prevention and treatment of cysticercosis valuable new vaccine

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  • Method for preparing and identifying recombinant bifidobacterium vaccine of taenia solium TSO45W-4B gene
  • Method for preparing and identifying recombinant bifidobacterium vaccine of taenia solium TSO45W-4B gene
  • Method for preparing and identifying recombinant bifidobacterium vaccine of taenia solium TSO45W-4B gene

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Embodiment Construction

[0019] The preparation and identification method of the T. solium TSO45W-4B gene recombinant bifidobacterium vaccine of the present invention comprises the following steps:

[0020] (1) TSO45W-4B gene synthesis and primer design: According to the TSO45W-4B gene sequence of Taenia solium, a full-length splicing primer was designed by the method of whole gene synthesis, and BamHI and EcoRI restriction sites and protective base. The TSO45W-4B gene with a length of 351bp was synthesized by Shanghai Handsome Biotechnology Co., Ltd.

[0021] (2) Construction and identification of the recombinant plasmid pGEX-TSO45W-4B: The PCR product of the synthetic gene and the cloning vector pMD18-T were ligated overnight at 16°C under the action of T4 DNA ligase, and the ligated product was transformed into E.coli Top10 competent cells. Ice bath for 30 min, 42 °C water bath for 90 s, after cooling in the ice bath, add 900 μl LB culture solution, shake at 37 °C 225 rpm for 45 min, centrifuge, d...

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Abstract

The invention discloses a method for preparing and identifying a recombinant bifidobacterium vaccine of a taenia solium TSO45W-4B gene. The taenia solium TSO45W-4B gene is synthetized by a complete genome, and directionally cloned to an escherichia coli-bifidobacterium expression vector pGEX-1lambdaT; a recombinant plasmid pGEX-TSO45W-4B is built; bifidobacterium longum (B.longum) is transformed in a manner of electroporation, and a taenia solium recombinant Bb(pGEX-TSO45W-4B) vaccine is built. Thus, a valuable novel vaccine is provided for prevention and treatment of cysticercosis cellulosae.

Description

technical field [0001] The invention relates to a preparation and identification method of a TSO45W-4B gene recombinant bifidobacterium vaccine of Taenia solium. Background technique [0002] Cysticercosis cellulosae (Cysticercosis cellulosae), also known as Cysticercosis (Cysticercosis), is a serious hazard caused by the middle-stage larvae of Taenia solium parasitizing in human subcutaneous, muscle, brain, eyes and other organs The zoonotic parasitic diseases of human health have become a global public health problem. my country is also one of the countries with a high incidence of porcine cysticercosis. The incidence is relatively high in the northeast, north, northwest, and southwest regions. There are about 2 million to 1 million people in the country There are 3 million cysticercosis patients, and the infection rate is 0.14% to 3.20%. Drugs and surgical treatment have their limitations, and the development of vaccines to prevent and treat the disease has become a curren...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/00C12N1/21C12N15/74C12N15/12C12Q1/68A61P33/10
Inventor 周必英
Owner ZUNYI MEDICAL UNIVERSITY
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