Method for rapidly preparing antrodia camphorate asexual spores

An asexual spore, rapid technology, applied in the field of bioengineering, can solve the problems of long cultivation period of Antrodia spores, and achieve the effect of shortening the cultivation period

Active Publication Date: 2014-03-26
SHENZHEN RENTAI BIOLOGICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The object of the present invention is to provide a kind of method for rapidly preparing the asexual spores of Antrodia cinnamomea, to solve the problem that the spores of Antrodia cinnamomea has a long culture cycle

Method used

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  • Method for rapidly preparing antrodia camphorate asexual spores
  • Method for rapidly preparing antrodia camphorate asexual spores
  • Method for rapidly preparing antrodia camphorate asexual spores

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] S1: pick the mycelia or spores of Antrodia camphorata and inoculate them in the center of the agar plate medium, place them at 28°C for 60 hours. The composition of the agar plate medium is 2% corn starch, 1% malt extract, 0.1% peptone, 0.01% camphor tree essential oil and 1.5% agar powder, and the balance is water.

[0027] S2: Cut off the part with mycelium growing on the agar plate, and crush it, put the debris into a 1000ml Erlenmeyer flask containing 200ml of sporulation medium, and place it in the culture flask with a diameter of about 3-5mm 10 small glass balls. The composition of sporulation medium is 1% corn starch, 2.2% bran, 1% malt extract, 0.1% peptone, 0.1% glucose, and the balance is water.

[0028] S3: Place the Erlenmeyer flask inoculated with the mycelium of Antrodia camphorata on a constant temperature shaking shaker, and cultivate in stages: the first stage: 28°C, 200rpm for 72 hours;

[0029] The second stage: adjust the temperature of the shaking...

Embodiment 2

[0033] S1: pick the mycelia or spores of Antrodia camphorata (the deposit number is CCTCC M2013359) and inoculate them in the center of the agar plate medium, and culture them at 25°C for 72 hours. The composition of the agar plate medium is 2% corn starch, 1% malt extract, 0.1% peptone, 0.01% camphor tree essential oil and 1.5% agar powder, and the balance is water.

[0034] S2: Cut off the part with mycelium growing on the agar plate, and crush it, put the debris into a 500ml Erlenmeyer flask containing 100ml of sporulation medium, and place it in the culture flask with a diameter of about 3-5mm 15 small glass balls. The composition of sporulation medium is 1% corn starch, 2.2% bran, 1% malt extract, 0.1% peptone, 0.1% glucose, and the balance is water.

[0035] S3: Place the Erlenmeyer flask inoculated with the mycelium of Antrodia camphorata on a constant temperature shaking shaker, and cultivate in stages: the first stage: 25°C, 180rpm for 60 hours;

[0036] The second ...

Embodiment 3

[0040] S1: pick the mycelia or spores of Antrodia camphorata (the deposit number is BCRC37849) and inoculate them on the center of the agar plate medium, and culture them at 26°C for 70 hours. The composition of the agar plate medium is 2% corn starch, 1% malt extract, 0.1% peptone, 0.01% camphor tree essential oil and 1.5% agar powder, and the balance is water.

[0041] S2: Cut off the part with mycelium growing on the agar plate, and crush it, put the debris into a 1000ml Erlenmeyer flask containing 200ml of sporulation medium, and place it in the culture flask with a diameter of about 3-5mm 20 small glass balls. The composition of sporulation medium is 1% corn starch, 2.2% bran, 1% malt extract, 0.1% peptone, 0.1% glucose, and the balance is water.

[0042] S3: Place the Erlenmeyer flask inoculated with the mycelium of Antrodia camphorata on a constant temperature shaking shaker, and cultivate in stages: the first stage: 26°C, 190rpm for 68 hours;

[0043] The second stag...

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Abstract

The invention belongs to the field of bioengineering and provides a method for rapidly preparing antrodia camphorate asexual spores. The method is based on the liquid submerged fermentation technology, through specially-owned fermentation medium and fermentation temperature control, antrodia camphorate is made to accumulate mycelia in the early-stage fermentation process, and the mycelia are rapidly converted into the asexual spores under the unfavorable environmental stress in the later stage. The cultivation period of the spores is greatly shortened through a temperature induction stress method, so that the problem that the period of the antrodia camphorate asexual spores which are prepared through liquid fermentation is long in the prior art is solved.

Description

technical field [0001] The invention relates to the field of bioengineering, in particular to a method for rapidly preparing asexual spores of Antrodia antrodia based on liquid submerged fermentation and temperature-induced stress. Background technique [0002] Antrodia cintrodia is a precious medicinal fungus in Taiwan. Antrodia cintrodia is plate-shaped or bell-shaped, with a salmon-red surface. It is used for health care, and its effect is far better than that of Ganoderma lucidum. It only grows in high-altitude evergreen broad-leaved trees. On the camphor tree. Antrodia Antrodia is rich in triterpenoids, superoxide dismutase, adenosine, polysaccharides, β-D-glucan, vitamins, immunoglobulins, etc. It is often used for anti-cancer, anti-itch, anti-allergy and anti-fatigue. In traditional therapy, Antrodia Cinnamomea is known as a "Holy Medicine for Anticancer". [0003] At present, the main methods of artificial cultivation of Antrodia camphorata include liquid fermentat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/04
Inventor 赵宗杰李光飞谢海涛周荣灵
Owner SHENZHEN RENTAI BIOLOGICAL TECH
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