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Nanoparticles loaded with neurotrophic factors, and preparation and applications thereof

A technology of neurotrophic factors and nano-microspheres, which can be applied to medical preparations with non-active ingredients, medical preparations containing active ingredients, and nervous system diseases, etc., and can solve problems such as easy degradation, short half-life, and poor self-aggregation and absorption , to achieve the effects of good biocompatibility, extended half-life, and strong factor release function

Inactive Publication Date: 2015-04-15
NANTONG UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Neurotrophic factors themselves have the disadvantages of short half-life, easy degradation in vivo, self-aggregation and poor absorption, so continuous administration of effective doses is the key to the success of constructing bioactive neural grafts

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Example 1: Preparation of ε-polylysine-heparin nanospheres

[0015] Add 1 mL of ε-polylysine (3 mg / mL) aqueous solution to 2 mL of heparin (3 mg / mL) aqueous solution with stirring at room temperature and stir for 1 h. Then, 2.3 mg of genipin, a biocompatible cross-linking agent, was added to carry out the cross-linking reaction for 12 hours. After the reaction is completed, an aqueous ε-polylysine-heparin nanosphere dispersion is formed. The particle size range of the nano microsphere is 180-350nm.

Embodiment 2

[0016] Example 2: Preparation of ε-polylysine-heparin nanospheres loaded with nerve growth factor (NGF)

[0017] Add 1 mL of ε-polylysine (3 mg / mL) aqueous solution containing 1 μg nerve growth factor (NGF) to 2 mL of heparin (3 mg / mL) aqueous solution and stir for 1 h at room temperature. Then, 2.3 mg of genipin, a biocompatible cross-linking agent, was added to carry out the cross-linking reaction for 12 hours. After the reaction is completed, the epsilon-polylysine-heparin nanometer microsphere dispersion liquid loaded with neurotrophic factors is formed. The particle size range of the nano microsphere is 180-350nm. The added amount of nerve growth factor matches the amount required for treatment, the same below.

Embodiment 3

[0018] Example 3: Preparation of ε-polylysine-heparin nanospheres loaded with basic fibroblast growth factor (bFGF)

[0019] Add 1 mL of ε-polylysine (3 mg / mL) aqueous solution containing 1 μg of basic fibroblast growth factor (bFGF) to 2 mL of heparin (3 mg / mL) aqueous solution with stirring at room temperature and stir for 1 h. Then, 2.3 mg of genipin, a biocompatible cross-linking agent, was added to carry out the cross-linking reaction for 12 hours. After the reaction is completed, a neurotrophic factor-loaded ε-polylysine-heparin nanosphere dispersion is formed. The particle size range of the nano microsphere is 180-350nm.

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Abstract

The invention discloses nanoparticles loaded with therapeutic factors or neurotrophic factors. The nanoparticles are made of a high molecular material; the high molecular material is composed of biocompatible positively charged epsilon-polylysine and negatively charged heparin; the mass ratio of epsilon-polylysine to heparin ranges from 1:20 to 1:1; and average particle size grain diameter of the nanoparticles ranges from 100 to 400nm. The epsilon-polylysine-heparin nanoparticles are taken as carriers for loading of neurotrophic factors, and are high in biocompatibility, and stable in biochemical properties. The nanoparticles loaded with neurotrophic factors are capable of promoting cell axon growth effectively via sustained release.

Description

technical field [0001] The invention relates to a biodegradable biomacromolecule nano-microsphere, which is used as a factor slow-release carrier, its preparation and application. Background technique [0002] Peripheral nerve injuries caused by traffic accidents, wars or industrial production accidents are very common traumas in clinical practice. Peripheral nerve damage usually results in permanent motor and sensory dysfunction in the innervated area. Clinically, after peripheral nerve injury, end-to-end adventitial suture is usually used to repair the nerve injury. When nerve damage cannot be sutured end-to-end, it is necessary to rely on nerve grafts to achieve the purpose of repairing nerve damage. However, the sources of autologous nerves are limited, the donor site is permanently denervated, and the allogeneic nerve has the problem of immune rejection, so it is necessary to study artificial nerve grafts to replace autologous nerve grafts. At present, biodegradable ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K38/18A61K47/36A61K47/34A61K9/16A61P25/00
Inventor 张鲁中杨宇民李贵才王彩萍
Owner NANTONG UNIVERSITY
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