Hydrogel of poly-beta amino ester loaded with active substance as well as preparation method and application of hydrogel

A technology of active substances and biologically active substances, applied in the field of hydrogel preparation, can solve the problems of mechanical properties of polypeptide hydrogels without biological activity, toxicity, functional modification problems, etc., to achieve stable chemical properties and biocompatibility Good and strong effect of drug sustained release function

Pending Publication Date: 2021-12-28
NANTONG UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these hydrogels suffer from the potential toxicity of cross-linking agents from natural materials, potential host reactions from acellular matrices, control of mechanical properties of peptide hydrogels, and the inactivity and difficulty of functionalization of polyethylene glycol gels during use. The problem of modification

Method used

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Examples

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preparation example Construction

[0027] A kind of preparation method of the hydrogel of the polyurethane beta amino ester that is loaded with active substance, comprises the following steps:

[0028] S1. Preparation of polybeta amino ester containing propyloyl group; stirring and reacting propyloyl hydrazide and polyethylene glycol diacrylate at 50-90 degrees Celsius for 12-72 hours, and dialysis through a dialysis bag with a molecular weight cut-off of 1000 to remove unreacted small molecules, and lyophilized to obtain the polybeta amino ester containing propioloyl group; the molar ratio of propiolyl hydrazide to polyethylene glycol diacrylate is 1:0.8-1.2:1.

[0029] S2. At room temperature, mix the bioactive substance with propioloyl-containing poly-beta amino ester evenly, dissolve it in distilled water, then add multi-arm mercapto PEG or inositol hexamercaptopropionate, and let it stand for 24 hours to obtain the carrier Hydrogel of polyurethane with active substance. The biologically active substance i...

Embodiment 1

[0031] Synthesis of Poly(β-Amino Ester) Containing Propioyl Group

[0032] Propigyl hydrazide (0.1 mmol) and polyethylene glycol diacrylate (0.1 mmol, Mw600) were stirred and reacted at 70 degrees Celsius for 24 hours, and unreacted small molecules were removed by dialysis through a dialysis bag with a molecular weight cut-off of 1000, and freeze-dried Poly([beta]-amino esters) containing propyne hydrazide are obtained.

Embodiment 2

[0034] Preparation of poly(β-amino ester) hydrogel

[0035] Poly(β-amino ester) (68.4 mg) containing propynyl hydrazide (68.4 mg) was dissolved in 6 mL of distilled aqueous solution, and inositol hexamercaptopropionate (26.1 mg) was added and mixed uniformly. After standing still for 24 hours, a poly(β-amino ester) hydrogel was obtained, which has self-healing properties.

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Abstract

The invention discloses hydrogel of poly-beta amino ester loaded with active substances as well as a preparation method and application of the hydrogel. A Michael addition reaction is carried out on polyethyleneglycol diacrylate and propargyl hydrazide to obtain poly-beta amino ester containing propiolyl; and a coupling reaction is carried out on a bioactive substance containing double bonds or sulfydryl, the poly-beta amino ester and PEG of multi-arm sulfydryl or inositol hexa-mercaptopropionate to obtain the hydrogel of the poly-beta amino ester loaded with the active substance. The hydrogel disclosed by the invention is good in biocompatibility and stable in chemical property, and can be used as a neurotrophic factor carrier for treating nerve injury.

Description

technical field [0001] The invention relates to a poly-beta amino ester hydrogel loaded with active substances, a preparation method and application thereof, and belongs to the technical field of hydrogel preparation. Background technique [0002] Spinal cord injury is a severe central nervous system injury disease, which will cause serious dysfunction of the limbs below the injury, such as partial or complete loss of sensory and motor functions. Spinal cord injury can also cause deep vein thrombosis, pulmonary embolism, pain and other complications, which seriously affect people's quality of life and bring heavy burdens to families and society. The causes of spinal cord injury include traffic accidents, heavy objects, falling from heights, etc. According to statistics, there are 200,000 new cases of spinal cord injury every year in the world. [0003] First of all, after spinal cord injury, trauma at the injured site will cause a series of secondary pathophysiological reac...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/52A61L27/54A61L27/18C08G65/333C08J3/075C08L71/02
CPCA61L27/52A61L27/54A61L27/18C08G65/33324C08J3/075A61L2430/32A61L2300/414A61L2300/602A61L2300/43C08J2371/02C08J2471/02C08L71/02
Inventor 张鲁中傅泽茜杨宇民李贵才魏静静李文慧
Owner NANTONG UNIVERSITY
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