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Preparation method and application of hyacinth bletilla hemostatic gel

A technology of hemostatic gel and bletilla striata, which is applied in surgery, medical science, etc., can solve the problems that bletilla striata can't exert good effect on hemostasis and antibacterial effect, and achieve the effect of good biocompatibility and promoting cell growth

Active Publication Date: 2014-03-26
张家界欣农农业科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, only using one component of Bletilla striata to prepare Bletilla striata hydrogel can not make Bletilla striata exert good hemostatic and antibacterial effects

Method used

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  • Preparation method and application of hyacinth bletilla hemostatic gel
  • Preparation method and application of hyacinth bletilla hemostatic gel

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] Take fresh bletilla striata, remove the root and wash it, treat it with high temperature and high pressure for 20 minutes, mash it and put it in canvas, wash it with water at 30-45°C, collect the filtrate, let it stand to collect the precipitate, dry it at low temperature at 20-40°C, and set it aside.

[0052] Weigh 2.0g hyaluronic acid (100,000, injection grade, bio-fermentation) into 200mL sterile three-distilled water, stir until dissolved, add 4.0g 2,2'-dithiodiamide, and then use 1.0mol / L HCl Adjust the pH to 3.0, add 1.5g EDC and stir for 2h, then adjust the pH to 7.0 with 1.0mol / L NaOH, add 5g of β-mercaptoethanol, adjust the pH to 8.0 with 1.0mol / L NaOH, stir for 12h, and use 1.0mol / L Adjust the pH to 2.0 with HCl, put it into a dialysis bag of Mw3500Da, dialyze in 0.01mol / L HCl (pH, 2.0) of 5gNaCl for 1 day, dialyze in 0.01mol / L HCl (pH, 2.0) solution for 1 day, centrifuge, and The supernatant liquid was freeze-dried to obtain 1.85 g of thiolated hyaluronic aci...

Embodiment 2

[0055] Take fresh bletilla striata, remove the root and wash it, treat it under high temperature and high pressure for 30 minutes, mash it and put it in canvas, wash it with water at 30-45°C, collect the filtrate, let it stand to collect the precipitate, dry it at low temperature at 20-40°C, and set it aside.

[0056] Weigh 2.0g of hyaluronic acid (500,000, injection grade, biological fermentation) into 250mL sterile three-distilled water, stir until dissolved, add 2.5g of 4,4'-dithiodiacetamide, and then adjust with 1.0N HCl pH to 4.0, add 2.5g EDC and stir for 2h, then adjust the pH to 7.0 with 1.0mol / L NaOH, add 15g of β-mercaptoethanol, adjust the pH to 8.5 with 1.0mol / L NaOH, stir for 12h, use 1.0mol / L Adjust the pH to 3.0 with HCl, perform ultrafiltration with ultrafiltration (Ningbo Hongyao Electrophoresis Technology Co., Ltd., the molecular weight cut-off is 1500Da), ultrafilter in 0.001mol / L HCl (pH, 3.0) containing 10g NaCl, and then filter in 0.001 mol / L HCl (pH, 3.0)...

Embodiment 3

[0059] Take fresh bletilla striata, remove the root and wash it, treat it with high temperature and high pressure for 25 minutes, mash it and put it in canvas, wash it with water at 30-45°C, collect the filtrate, let it stand to collect the precipitate, dry it at low temperature at 20-40°C, and set it aside.

[0060] Weigh 2.0g of hyaluronic acid (800,000, injection grade, bio-fermentation) into 300mL sterile three-distilled water, stir until dissolved, add 3.0g of 2,2'-dithiodiacetylhydrazide, and then add 1.0mol / L HCl to adjust the pH to 4.5, add 3.0g EDC and stir for 2h, then use 1.0mol / L NaOH to adjust the pH to 7.4, add 20g of β-mercaptoethanol, use 1.0mol / L NaOH to adjust the pH to 8.5, stir, 24h, use 1.0mol / L HCl to adjust the pH to 3.5, put it into a dialysis bag of Mw3500Da, dialyze in 15g NaCl (pH, 3.5) for 2 days, and dialyze in HCl (pH, 3.5) for 2 days, centrifuge, and freeze-dry the supernatant to obtain 1.85g The mercapto-hyaluronic acid was qualitatively and qu...

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Abstract

The invention relates to a mixed preparation method of hyacinth bletilla effective components and a gel, and particularly relates to a method for preparing a hemostatic gel through polymerization of a hyacinth bletilla extract and hyaluronic acid and application of the hemostatic gel. Hyacinth bletilla and hyaluronic acid are used as raw materials, so that the hydrogel has high elasticity and certain mechanical strength and facilitates release of the hyaluronic acid, thereby ensuring that the hydrogel can be used as an injection hydrogel. By regulating the amount of the hyacinth bletilla and the concentration of the sulfhydrylated hyaluronic acid, hydrogels with different mechanical parameters can be obtained, thereby obtaining the hydrogel having a hemostatic function. The hyaluronic acid is an in vivo inherent component, can promote growth of cells and is fine in biocompatibility. The hyaluronic acid in the hydrogel obtained by the invention is easier to dissociate.

Description

technical field [0001] The invention belongs to the technical field of polymer materials, and relates to a preparation method for mixing active ingredients of bletilla striata and gel, in particular to a method and application of preparing hemostatic gel by polymerizing bletilla striata extract and hyaluronic acid. Background technique [0002] Bletilla striata, also known as Baiji and Ruolan, is a kind of orchid family, and its Latin name is Bletilla striata. Perennial herb, pseudobulb tuberous, white, plump, with finger-like divergences. The stem is stout, erect, 30-60 cm high. It is mainly used for hemoptysis and hematemesis, traumatic bleeding, sores and swollen toxins, and chapped skin. Tuberculosis hemoptysis, ulcer disease bleeding. Its corm contains bletilla striata gum, starch, volatile oil and so on. Medicinal, it has the effects of effect, nourishing the lungs, hemostasis, detumescence, etc. It can be applied externally to treat traumatic bleeding, carbuncle, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08J3/24C08J3/075C08L5/00C08L5/08C08F251/00C08F220/06C08B37/08A61L31/16A61L31/14A61L31/04
Inventor 唐忠海
Owner 张家界欣农农业科技有限公司
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