A kind of radiation degradation treatment method of fumonisin and t-2 toxin

Abstract

The invention relates to an irradiation degradation processing method of fumitremorgin and T-2 toxin. The irradiation degradation processing method comprises the steps of (1) preparing standard solutions for standard substances of fumitremorgin FB1 and the T-2 toxin, carrying out irradiation processing with the irradiation range of 0-200kGy on the standard solutions, and measuring the contents of the FB1 and T-2 through a liquid chromatogram-tandem mass spectrometry; (2) carrying out preprocessing on samples containing poison after irradiation with the irradiation range of 0-9kGy, and measuring the FB1 and T-2 degrading effects through a liquid chromatogram-tandem mass spectrometry; (3) selecting the FB1 and T-2 standard solutions with highest concentrations as the samples to carry out irradiation processing with the irradiation range of 0-200kGy, and then detecting and analyzing degradation products through the liquid chromatogram-tandem mass spectrometry. The irradiation degradation processing method provided by the invention has the advantages of realizing research and environment-friendly application of the FB1 and T-2 degradation product which lacks of irradiation degradation, the degradation effect is good, and the environment-friendly detoxification processing of mildew agricultural products waste is realized.

Description

technical field [0001] The invention belongs to the field of analytical chemistry, and in particular relates to an irradiation degradation treatment method of fumonisin and T-2 toxin. Background technique [0002] Fumonisin and T-2 belong to mycotoxins. Mycotoxins are toxic metabolites produced by toxin-producing fungi under suitable environmental conditions. Fumonisin and T-2 are particularly harmful to humans and have a high frequency of pollution. Strict limit standards have been established for fumonisin and T-2 in grains, food and feed. [0003] In Chinese patent 201110000805.2 "Method for detecting ochratoxin A in traditional Chinese medicine liquor by immunoaffinity column purification by high performance liquid chromatography", it is disclosed that positive results are confirmed by high performance liquid chromatography-mass spectrometry; in Chinese patent 201010209190 .X "A method for detecting nivalenol and deoxynivalenol toxins in traditional Chinese medicine", d...

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Problems solved by technology

[0004] Existing related technologies include: Zhang Xichun et al. reported that the irradiation dose of 3kGy~5kGy has a more obvious effect on the degradation of mold and mycotoxins; The degradation rate can reach more than 80% at 4kGy, 96% at 6kGy, and more than 90% at 10kGy of 0.1mg / L fumonisin B1 solution; Yin Qinggang's research results show that when the irradiation dose is 18kGy , the degradation rate of zearalenone in powdered corn can reach 74.9%. When the irradiation dose is 10kGy, the degradation rate of zearalenone in whole corn can reach 94.1%. When the irradiation dose is 5kGy, 30.0mg The degradation rate of zearalenone / kg zearalenone aqueous solution reached 90%; Chi Lei et al. published a paper on the degradation of ochratoxin A by gamma rays, which mentioned the method of structural analysis of degradation products and the possible mechanism of degradation , but no degradation products were reported

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