Method for determining content of hydrogen peroxide by determining concentration of oxygen

A technology for hydrogen peroxide and oxygen concentration, which is used in measuring devices, instruments, scientific instruments, etc., can solve the problems of insignificant color change at the end point, many interference factors, low sensitivity, etc., and achieves short measurement process time and strong anti-interference ability. , the effect of high sensitivity

Active Publication Date: 2014-04-09
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Among them, the iodometric method is the most widely used hydrogen peroxide determination method in factory practice. This method is simple to operate, but has low sensitivity and many interference factors.

Method used

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  • Method for determining content of hydrogen peroxide by determining concentration of oxygen
  • Method for determining content of hydrogen peroxide by determining concentration of oxygen
  • Method for determining content of hydrogen peroxide by determining concentration of oxygen

Examples

Experimental program
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Effect test

Embodiment 1

[0033] The mensuration of hydrogen peroxide content in the urea peroxide of embodiment 1

[0034] (1) Preparation of hydrogen peroxide standard solution: Accurately measure 2.0mL of 30% hydrogen peroxide solution by mass percentage, add deionized water to dilute to 100mL, and then carry out chemical calibration according to the national standard GB6684-2002.

[0035] (2) Preparation of enzyme solution: Accurately weigh 0.4g of catalase, add phosphate buffer to dissolve, and then dilute to 100mL.

[0036] (3) Establish a standard curve: Add 3.0mL enzyme solution to a series of headspace vials, seal the vials, and then add 0, 0.1, 0.25, 0.5, 0.75, 1.0 , 1.5mL hydrogen peroxide standard solution, put the sample bottle in a water bath, and after reacting at 37°C for 5 minutes, place it on the sample stage, and then perform headspace-gas chromatography detection, and obtain the peak area of ​​the oxygen signal The corresponding relationship between the amount of hydrogen peroxide ...

Embodiment 2

[0041] The mensuration of residual hydrogen peroxide content in the hydrogen peroxide bleaching waste liquor of embodiment 2

[0042] (1) Preparation of hydrogen peroxide standard solution: Accurately measure 2.0mL of 30% hydrogen peroxide solution by mass percentage, add deionized water to dilute to 100mL, and then carry out chemical calibration according to the national standard GB6684-2002.

[0043] (2) Preparation of enzyme solution: Accurately weigh 0.4g of catalase, add phosphate buffer to dissolve, and then dilute to 100mL.

[0044] (3) Establish a standard curve: Add 3.0mL enzyme solution to a series of headspace vials, seal the vials, and then add 0, 0.1, 0.25, 0.5, 0.75, 1.0 , 1.5mL hydrogen peroxide standard solution, put the sample bottle in a water bath, and after reacting at 37°C for 5 minutes, place it on the sample stage, and then perform headspace-gas chromatography detection, and obtain the peak area of ​​the oxygen signal The corresponding relationship betw...

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Abstract

The invention relates to a method for determining the content of hydrogen peroxide by determining the concentration of oxygen. The method comprises the following steps: (1) preparing a standard solution; (2) preparing an enzyme solution; (3) establishing a standard curve; (4) performing sample detection by adopting headspace-gas chromatography; and (5) performing result calculation. According to the method provided by the invention, the content of the hydrogen peroxide is determined by utilizing a way combining a biological enzyme with headspace-gas chromatography. The method has the beneficial effects that the method is adopted for determining the content of the hydrogen peroxide, and the method has the advantages of strong specificity, high accuracy, convenience, rapidness, short determination time, strong anti-interference capability and the like, and is suitable for batch detection of samples.

Description

technical field [0001] The invention relates to the technical field of hydrogen peroxide content detection, in particular to a method for measuring hydrogen peroxide content by using biological enzymes combined with headspace-gas chromatography. Background technique [0002] hydrogen peroxide (H 2 o 2 ) Commonly known as hydrogen peroxide, it is a colorless liquid with strong oxidizing and corrosive properties. It can be used as a disinfectant, dechlorination agent, bleaching agent, oxidant and polymerization initiator, etc. Medicine, papermaking, national defense and other fields. Accurate determination of hydrogen peroxide content is of great significance to improve the efficiency of hydrogen peroxide and control its reaction process. [0003] At present, the methods for detecting hydrogen peroxide mainly include the following categories: conventional titration, spectrophotometry, chemiluminescence, fluorescence photometry, chromatography, enzymatic chemistry, electroch...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
Inventor 李海龙
Owner SOUTH CHINA UNIV OF TECH
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