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A rapid extraction method of curcuma longa dna

PendingCN122303220ASimple and fast operationReduce inhibitionBiotechnologyMedicinal herbs
This invention discloses a rapid method for extracting DNA from turmeric, belonging to the fields of plant molecular biology and nucleic acid extraction technology. The method includes: taking a turmeric sample, grinding, crushing, or homogenizing it, adding nucleic acid lysis buffer and Chelex 100 resin filler, mixing thoroughly, and then heating to lyse the sample, causing the turmeric tissue cells to release DNA; subsequently, removing tissue debris, resin particles, polysaccharides, pigments, and other insoluble impurities by filtration or centrifugation, and collecting the filtrate or supernatant to obtain the initial extracted turmeric DNA. This method is simple to operate, fast in extraction, and low in cost. It does not require organic reagents such as phenol and chloroform, and the obtained DNA can be directly used for PCR amplification, DNA barcoding detection, molecular marker analysis, and identification of the authenticity of turmeric medicinal materials. It is suitable for rapid detection and batch processing of turmeric samples.
Owner:FUJIAN UNIV OF TRADITIONAL CHINESE MEDICINE