This invention discloses a rapid method for extracting
DNA from turmeric, belonging to the fields of
plant molecular biology and
nucleic acid extraction technology. The method includes: taking a turmeric sample,
grinding, crushing, or homogenizing it, adding
nucleic acid lysis buffer and Chelex 100 resin filler, mixing thoroughly, and then heating to lyse the sample, causing the turmeric tissue cells to release
DNA; subsequently, removing tissue debris, resin particles, polysaccharides, pigments, and other insoluble impurities by
filtration or
centrifugation, and collecting the filtrate or supernatant to obtain the initial extracted turmeric
DNA. This method is simple to operate, fast in extraction, and low in cost. It does not require organic reagents such as
phenol and
chloroform, and the obtained DNA can be directly used for PCR amplification,
DNA barcoding detection,
molecular marker analysis, and identification of the authenticity of turmeric medicinal materials. It is suitable for
rapid detection and
batch processing of turmeric samples.