The invention belongs to the technical field of detection tools, and relates to a diaphragm airtightness detection automation device and a use method thereof, the diaphragm airtightness detection automation device comprises a rack, a positioning tool, a detection mechanism and a conveying mechanism; the rack comprises an upper frame and a lower frame, the detection mechanism comprises a three-axisdisplacement table, a transverse support and a vacuum sucker, and the three-axis displacement table is installed in the upper frame; a transverse bracket and a vacuum chuck are mounted on the three-axis displacement table; the positioning tool comprises an air cylinder, polished shafts, a pressing plate, a connecting plate, lower jigs and upper jigs; the air cylinder is installed in the lower frame, the connecting plate is arranged on a piston rod of the air cylinder, the two ends of the connecting plate are fixedly connected with the polished shafts, the pressing plate is fixedly arranged atthe tops of the polished shafts, and the multiple upper jigs are arranged at the bottom of the pressing plate. A plurality of lower jigs are arranged at the top of the lower frame, the two sides of the diaphragm are covered with the upper jigs and the lower jigs, just like air holes communicated with the outside are formed in the lower jigs in the upper jigs, vacuumizing operation is further conducted on air in the upper jigs, and then the air tightness of the diaphragm can be detected.