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Liquid flow system of flow cytometer, air tightness detection method and flow cytometer

A flow cytometer and air tightness technology, which is applied in the field of flow cytometer, can solve the problems of poor clogging detection accuracy, poor instrument reliability, poor practicability of instruments, and poor maintenance of flow cytometer, and achieves simple and convenient operation and convenience. The effect of blockage removal maintenance and reliability improvement

Inactive Publication Date: 2019-08-13
北京唯公医疗技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In traditional flow cytometers, only a small number of configurations have the functions of liquid path air tightness and blockage alarm, but the accuracy of liquid path air tightness detection and blockage detection is poor, and the reliability and practicability of the instrument are often relatively low. Poor, resulting in poor maintainability of the flow cytometer

Method used

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  • Liquid flow system of flow cytometer, air tightness detection method and flow cytometer

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Experimental program
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Embodiment 1

[0082] This embodiment provides a method for detecting air tightness of the liquid flow system 10 of a flow cytometer, which includes the following steps:

[0083] see figure 1 As shown, the liquid flow system 10 is in an initialized state to be tested;

[0084] Close the emptying valve 120, the filter exhaust valve 380, the sample control valve 230, the first sheath fluid control valve 320, the second sheath fluid control valve 330 and the negative pressure control valve 440; A certain volume of sheath fluid is pumped in or out to make the pressure reach 30 kPa, and the pressure value P2 of the sheath fluid pressure sensor 350 is read after 10 seconds. If |P2-P1|<0.5kPa, it means that the airtightness of the sheath liquid device 300 of the liquid flow system 10 is good, and if |P2-P1|≥0.5kPa, it means that the airtightness of the sheath liquid device 300 of the liquid flow system 10 is not good. it is good;

[0085] In the initialization state, read the pressure value P7 o...

Embodiment 2

[0089] This embodiment provides a method for detecting air tightness of the liquid flow system 10 of a flow cytometer, which is easy to operate, has high detection accuracy, and high detection efficiency.

[0090] The liquid flow system 10 air tightness detection method of the above-mentioned flow cytometer comprises the following steps:

[0091] see figure 1 As shown, the filter exhaust valve 380, the sample control valve 230, and the first sheath liquid control valve 320 of the liquid flow system 10 are opened, so that the sheath liquid device, the test device, and the sample loading device of the entire liquid circuit system are filled with sheath liquid. At this time, the liquid flow system 10 is in an initialized state to be tested;

[0092] Close the emptying valve 120, the sample control valve 230, the filter exhaust valve 380, the first sheath fluid control valve 320, the second sheath fluid control valve 330 and the negative pressure control valve 440;

[0093] Pump...

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Abstract

The invention discloses a liquid flow system of a flow cytometer, an air tightness detection method and a flow cytometer. The liquid flow system comprises a sheath liquid device, a negative pressure device, a testing device and a sample loading device. The sheath liquid device comprises a sheath liquid pipeline communicated with the liquid inlet, a first sheath liquid control valve arranged on thetank flushing pipeline, a sheath liquid pump arranged on the sheath liquid pipeline, a sheath liquid pressure sensor and a second sheath liquid control valve, and the tank flushing pipeline is also communicated with the sheath liquid pipeline; the negative pressure device is communicated with the flow chamber and used for generating negative pressure for the liquid flow system; the testing devicecomprises a flow chamber and a negative pressure control valve arranged at a liquid outlet of the flow chamber; and the sample loading device comprises a sample loading pipeline communicated with a sample loading opening of the flow chamber, a sampling part arranged on the sample loading pipeline and a sample control valve. The liquid flow system can improve the accuracy of liquid path air tightness detection and blockage detection and reduce the cost of instruments.

Description

technical field [0001] The invention relates to the field of biological detection, in particular to a liquid flow system of a flow cytometer, an air tightness detection method and the flow cytometer. Background technique [0002] Flow cytometer (Flow Cytometer) quickly analyzes the physical or chemical properties of cells and other biological particles by measuring the scattered light and labeled fluorescence intensity, and can simultaneously measure multiple characteristic parameters from a cell for qualitative or quantitative analysis. Analysis has the characteristics of fast speed, high precision and good accuracy. Flow cytometer is mainly composed of four parts: liquid flow system, optical system, electronic system and data analysis system. [0003] As the basic system of flow cytometer, the liquid flow system sends the sample liquid containing fluorescently stained cells or particles together with the sheath fluid into the flow chamber, and uses the principle of liquid...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N15/14G01M3/26
CPCG01M3/26G01N15/1404
Inventor 张洪祥李为公
Owner 北京唯公医疗技术有限公司
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