Peanut rhizosphere biocontrol bacterium, and preparation method and application thereof

A bio-control bacteria and peanut root technology, applied in the biological field, can solve the problem of less Brevibacillus lateralis, etc.

Inactive Publication Date: 2014-04-30
青岛乡润生物科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0013] There are few reports on the inhibitory mechanism o

Method used

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  • Peanut rhizosphere biocontrol bacterium, and preparation method and application thereof
  • Peanut rhizosphere biocontrol bacterium, and preparation method and application thereof
  • Peanut rhizosphere biocontrol bacterium, and preparation method and application thereof

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Embodiment Construction

[0039] 1. Isolation of active strain LX12

[0040] Weigh 5g of peanut rhizosphere soil in the experimental field of Shandong Peanut Research Institute, put it into a triangular flask with 45ml of distilled water, shake it for a while, absorb 0.1ml of the supernatant and add it to a test tube containing 4.5ml of sterile water to make a suspension with a concentration of 1:100. Then add 0.2ml of the soil suspension of West Zone 10-2 and 10-3 dropwise to the LB solid medium plate containing rifampicin, spread evenly with a coating rod, and place the medium upside down at 28°C Cultured in a constant temperature incubator for 2 days. Pick a batch of single colonies on the plate, draw a line on the LB solid medium plate and place it upside down in a constant temperature incubator at 28°C for 2 days.

[0041] Among them, after one day of line-drawing culture in LB medium, the colony is about 0.3mm, white, with neat edges, raised, smooth and moist surface, and opaque. Such as figur...

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Abstract

The invention belongs to the biotechnical field, and concretely relates to a peanut rhizosphere biocontrol bacterium, and a preparation method and an application thereof. The peanut rhizosphere biocontrol bacterium is an active strain LX12, the strain LX12 is identified as Brevibacillus laterosporus through 16SrDNA, and the strain is a bacterium screened from peanut rhizosphere soil, antagonistic to fungi, is preserved in China General Microbiological Culture Collection Center, and has a preservation number of CGMCC7420. The strain has an obvious antagonistic effect on pathogens, standoff tests show that the bacterium has a substantial inhibition effect on peanut diseases comprising peanut root rot, southern blight, scab, Phyllosticta glycine and the like, and pot and field tests show that the bacterium has an obvious control effect on the peanut root rot, southern blight, scab, Phyllosticta glycine and the like.

Description

technical field [0001] The invention belongs to the field of biological technology, and in particular relates to a peanut rhizosphere biocontrol bacterium and its preparation method and application. Background technique [0002] In the research and application of bio-control bacteria, Bacillus and Pseudomonas are the two most deeply studied bio-control bacteria. Bacillus (Bacillaceae) is a family of bacteria, bacilli or cocci that can form spores (endospores). Including Bacillus, Lactobacillus, Clostridium, Desulfurized Enterobacter and Bacillus sarcina, etc. They are highly resistant to external harmful factors, widely distributed, and exist in soil, water, air, and animal intestines. The spore is a dormant body, and the vast majority is a bacterium that only forms a spore. The spore is located in the bacterium and consists of a core, a cortex, a spore shell and an outer wall. It is a Gram-positive bacterium. The core is the protoplast of the spore, which contains DNA, R...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12Q1/02A01N63/02A01P3/00C12R1/01
Inventor 迟玉成赵显民许曼琳许婷婷吴菊香王磊谢宏峰焦坤
Owner 青岛乡润生物科技有限公司
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