Immobilized method and application of petroleum degrading bacteria

A petroleum-degrading bacteria and a technology for petroleum-degrading are applied in the field of immobilization of petroleum-degrading bacteria, which can solve the problems of increasing operation complexity and operation cost, and achieve the effects of easy mass production, simple preparation process and low production cost.

Active Publication Date: 2014-05-07
CHINA PETROLEUM & CHEM CORP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the process of preparing immobilized bacteria, this invention needs to supplement the proliferation medium

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0025] Example 1

[0026] (1) Pretreatment of carrier material

[0027] The corn stalks, wheat stalks and sycamore leaves collected in the farmland and the field are dried, crushed, and passed through a 24-mesh sieve; then, they are mixed with commercially available wheat bran and homemade 24-mesh walnut shell activated carbon. The ratio of the carrier material is 50wt% wheat straw, 20wt% corn stalk, 5wt% wheat bran, 10wt% sycamore leaves, 12.5wt% walnut chitin activated carbon. The mixed carrier is soaked in distilled water for 12-24 hours, adjusted to pH 7.0-7.5, filtered off the floating impurities and supernatant, and the filtered mixed carrier is dried at 50°C for 24 hours, autoclaved at 121°C for 20 minutes, and set aside.

[0028] Walnut shell activated carbon uses ZnCl 2 Preparation by activation method, specifically as follows: Weigh a certain amount of crushed walnut shells that have passed through a 24-mesh sieve and soak in 50wt% ZnCl 2 In the solution, the ratio of mate...

Example Embodiment

[0036] Example 2

[0037] The difference from Example 1 is that the carrier material ratio is 37.5wt% wheat stalks, 20wt% corn stalks, 10wt% wheat bran, 12.5wt% sycamore leaves, 20wt% walnut shell activated carbon.

[0038] Under the same conditions for 40 days, the removal rate of petroleum pollutants reached 62wt%, of which the removal rate of saturated hydrocarbons could reach 72.5wt%.

Example Embodiment

[0039] Example 3

[0040] One of the differences from Example 1 is that the carrier material has a ratio of 25wt% wheat straw, 30wt% corn stover, 15wt% wheat bran, 15wt% sycamore leaves, 15wt% walnut shell activated carbon. The second difference is: the amount of the immobilized petroleum degrading bacteria product is 1kg / m 2 .

[0041] Other conditions are the same, the repair time is 40 days, the removal rate of petroleum pollutants reaches 58.5wt%, and the removal rate of saturated hydrocarbons can reach 70.3wt%.

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PUM

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Abstract

The invention discloses an immobilized method and application of petroleum degrading bacteria. The method includes the following steps: mixing and pretreating carrier materials of wheat straw, corn straw, wheat bran, leaves and walnut shell activated carbon; inoculating an activated petroleum degrading bacterium seed liquid, and carrying out adsorption culture; and filtering out an upper bacterial suspension under aseptic conditions, rinsing the carrier materials with sterile water, transferring the carrier materials adsorbed with bacteria to a new petroleum degrading bacterium seed liquid, repeating several times to reach saturation of the bacteria adsorbed by the carrier materials, so as to obtain the immobilized particles. The invention uses the natural organic materials and natural inorganic materials as carriers to overcome the defects of the individual usage of inorganic carrier or organic carrier; adsorption method is employed to realize immobilization of petroleum degrading bacteria; and the prepared immobilized petroleum degrading bacteria can be applied to in-situ remediation of petroleum contaminated soil to reach good effect, and is an environmentally friendly product which can be popularized and applied.

Description

technical field [0001] The invention relates to microorganism immobilization technology, in particular to an immobilization method and application of petroleum-degrading bacteria used for repairing petroleum-contaminated soil. Background technique [0002] During the process of oil extraction, refining, transportation and use, various reasons will cause different degrees of leakage, which will cause oil pollution to the soil. Petroleum-contaminated soil is one of the important pollutions of petroleum and petrochemical enterprises, and the petroleum pollutants in it have a high poisonous effect on human body and environment. The treatment methods of petroleum-contaminated soil at home and abroad mainly include incineration, landfill, stacking and bioremediation. [0003] The core of microbial remediation technology is the degradation of petroleum pollutants by highly efficient degrading bacteria. The degradation process makes the composition of pollutants tend to be more com...

Claims

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Application Information

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IPC IPC(8): C12N11/14C12N11/02B09C1/10
Inventor 秦丽姣邓德刚周彤
Owner CHINA PETROLEUM & CHEM CORP
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