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Method for quickly detecting polymorphism of cattle APOA2 gene and application thereof

A technology of polymorphism and scalping, which is applied in the direction of biochemical equipment and methods, microbial measurement/testing, etc., can solve problems such as blanks, and achieve the effects of low cost, reduced method and instability

Inactive Publication Date: 2014-05-14
NORTHWEST A & F UNIV
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0005] There is no research on the genetic variation of the cattle APOA2 gene at home and abroad, and the research on the function of this gene locus and the relationship between genetic variation and economic traits (such as body weight, etc.) are still blank

Method used

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  • Method for quickly detecting polymorphism of cattle APOA2 gene and application thereof
  • Method for quickly detecting polymorphism of cattle APOA2 gene and application thereof
  • Method for quickly detecting polymorphism of cattle APOA2 gene and application thereof

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Embodiment Construction

[0044] specific implementation plan

[0045] The present invention utilizes the PCR-RFLP method to detect the polymorphisms of the three loci of cattle APOA2 gene AC_000160.1g.360T>G, AC_000160.1g.424G>C and AC_000160.1g.540A>G. The detailed description is intended to explain rather than limit the present invention.

[0046] 1. Cloning of partial DNA sequence of cattle APOA2 gene and detection of its polymorphism

[0047] 1. Blood sample collection

[0048] In the present invention, a total of 1021 yellow cattle individuals of 4 Chinese local yellow cattle varieties are used as detection objects.

[0049] The specific collection samples are shown in Table 1:

[0050] Table 1 Sample Collection Information Form

[0051] Variety Number of samples Sample source Sampling method Nanyang cattle 225 Yellow Cattle Breeding Farm in Nanyang City, Henan Province jugular vein blood sampling Qinchuan cattle 246 Qinchuan Cattle Breeding Center of Shaanxi...

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Abstract

The invention discloses a method for quickly detecting PCR-RFLP (Polymerase Chain Reaction-Restriction Fragment Length Polymorphism) of sequence variation of Chinese native cattle APOA2 gene. The method comprises the following steps: taking the to-be-detected cattle whole-genome DNA (Desoxvribose Nucleic Acid) containing the APOA2 gene as a template and a primer pair P as the primers, and discovering three mutation sites by amplifying the APOA2 gene through PCR, designing three pairs of primers PR1, PR2 and PR2 for the three mutation sites, carrying out digestion of the PCR products by using three restriction endonucleases Bmgt120I, HindIII and HaeIII, respectively, and then identifying the polymorphism of the three mutation sites of the cattle APOA2 gene by virtue of agarose gel electrophoresis. Therefore, a method for DNA level screening and for identifying the polymorphism of a gene closely related to the growth traits of cattle is provided for marker assisted selection (MAS) breeding of the growth traits of the Chinese native cattle, and as a result, a cattle population excellent in genetic resources can be established quickly.

Description

technical field [0001] The invention belongs to the field of animal molecular breeding, and relates to a method for detecting the polymorphisms of yellow cattle APOA2 genes AC_000160.1g.360T>G, AC_000160.1g.424G>C and AC_000160.1g.540A>G, and its growth characteristics in beef cattle The application of marker-assisted selection (MAS) breeding to quickly establish cattle populations with excellent genetic resources. Background technique [0002] In the MAS breeding of cattle growth and development traits, there are two basic methods to study the relationship between DNA markers and target traits: candidate gene analysis and linkage marker analysis. In animal research, candidate gene analysis is easier to operate than linkage marker analysis, and does not require special experimental design, low cost, and strong statistical power. It is widely used and applicable to any population where genotype and phenotype data are available. The method establishes a corresponding...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
CPCC12Q1/686C12Q1/6888C12Q2600/124C12Q2600/156C12Q2535/138
Inventor 陈宏周扬李彩霞蔡含芳徐瑶蓝贤勇雷初朝
Owner NORTHWEST A & F UNIV
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