A specific molecular marker for detecting rice panicle structure gene ips1 and its detection method

A molecular marker, structural gene technology, applied in biochemical equipment and methods, microbial determination/inspection, DNA/RNA fragments, etc., can solve problems such as incompatibility, achieve optimal ratio, improve rice quality, and improve ear structure Effect

Inactive Publication Date: 2017-02-08
FARMING & CULTIVATION RES INST OF HEILONGJIANG ACADEMY OF AGRI SCI
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  • Abstract
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Problems solved by technology

These genes are not suitable for Northeast rice region

Method used

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  • A specific molecular marker for detecting rice panicle structure gene ips1 and its detection method
  • A specific molecular marker for detecting rice panicle structure gene ips1 and its detection method

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Embodiment Construction

[0032] The above and other technical features and advantages of the present invention will be described in more detail below in conjunction with the accompanying drawings.

[0033] The recombinant inbred line population constructed by the Northeast japonica rice 'Shennong 265' and the Yunnan local variety 'Lijiang Xintuan Heigu' of the present invention was analyzed in Shenyang and Harbin for the QTL analysis of panicle structural traits, and a total of control branches were detected. 5 QTLs for the number of peduncles were obtained, and the QTL IPS1, which controls both the number of primary peduncles and the number of solid grains of primary peduncles, was fine-mapped using the remaining heterozygous derived populations, and molecular markers that were closely linked and co-segregated with IPS1 were provided.

[0034] The specific molecular marker used to detect IPS1 in the present invention is IPS1-1, wherein the nucleotide sequence of the molecular marker of the present inv...

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Abstract

The invention relates to a specific molecular marker for detecting a structural gene IPS1 of rice spikes and a detection method thereof. The molecular marker is IPS1-1; and the fragment is shown in the sequence SEQ ID NO: 1. The method comprises the specific steps of a, DNA extraction; b, PCR (polymerase chain reaction) amplification: a PCR reaction system is 15 microlitres and contains 50mM of KCl, 10mM of Tris-HCl(pH8.8), 0.1 percent of Triton-X, 1.5mM of MgCl2, 200mu M of each of NTPs, 0.2mu M of each primer, 5 percent (v / v) dimethyl sulfoxide and 0.5UT of aq DNA polymerase; c, PCR product detection: adding sample injection buffer solution into an amplification product, adding 8 microlitres of the sample into 1.5% agarose gel, performing electrophoresis under constant voltage of 100V for 1 hour, and observing an image formed by a gel-imaging system; and d, obtaining the gene IPS1. The molecular marker disclosed by the invention can be used for authenticating whether a material to be detected contains a synergistic allele of a control spike structure of Lijiang new black rice on an IPS1 site; the spike structure can be improved, the ratio of kernels per spike of primary and secondary branches is optimized, the number of spike kernels is increased, the unit yield of paddy rice is enhanced, and the quality of rice is improved.

Description

technical field [0001] The invention relates to the technical field of detection in rice breeding, in particular to a specific molecular marker and a detection method for detecting IPS1 alleles that simultaneously increase yield and efficiency during molecular-assisted breeding of high-yield and high-quality rice. Background technique [0002] As the final yield organ of rice, the structure and shape of the panicle is always an important reference index in the breeding process, which involves two of the three elements of rice yield (number of panicles per plant, number of grains per panicle and grain weight). Due to ecological conditions and other reasons, the panicle number level of northeast japonica rice is relatively high, and the potential for further increase on this basis is small. Under long-term natural selection and artificial selection, there is little difference in thousand-grain weight among the varieties used in production, and the effect on high yield is relat...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6895C12Q2600/13
Inventor 姜树坤王嘉宇张凤鸣刘丹白良明孙世臣丁国华王彤彤张喜娟姜辉
Owner FARMING & CULTIVATION RES INST OF HEILONGJIANG ACADEMY OF AGRI SCI
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