A method for evaluating the antibacterial activity of Andrographis paniculata

A technology of antibacterial activity and Andrographis paniculata, applied in the field of evaluating the antibacterial activity of Andrographis paniculata, can solve the problems of large influence, low precision, and inability to track and record bacteria in real time, and achieve the effect of strong specificity and rapid detection.

Active Publication Date: 2016-01-13
THE FIFTH MEDICAL CENT OF CHINESE PLA GENERAL HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, these methods are complex and cumbersome to operate, are greatly affected by the external environment, and have low precision, and most of them are end-point design methods, which only compare the antibacterial activities of various drugs at the end, and cannot track and record the influence of bacterial growth and metabolism in real time. Characteristic Information of Changes Produced by Drug Intervention

Method used

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  • A method for evaluating the antibacterial activity of Andrographis paniculata
  • A method for evaluating the antibacterial activity of Andrographis paniculata
  • A method for evaluating the antibacterial activity of Andrographis paniculata

Examples

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Effect test

Embodiment 1

[0054] Weigh 20 g of Andrographis paniculata (produced in Xishuangbanna, Yunnan) into a round-bottomed flask, add deionized water whose volume (ml) is 10 times the weight of Andrographis paniculata (g), soak for 30 minutes, and reflux extraction twice. The reflux time is 2 hours, the extracts are combined and filtered with filter paper, the filtrate is decompressed to recover water, dried in vacuum to obtain a dry extract, and the yield is calculated. Wherein, the dry extract is the Andrographis paniculata sample.

Embodiment 2

[0056] Andrographis paniculata samples were prepared in the same way as in Example 1, except that the volume (ml) of deionized water was 5 times the weight (g) of the whole herb of Andrographis paniculata, the reflux time was 1.5 h and the reflux extraction was performed once.

Embodiment 3

[0058] Weigh 20 g of Andrographis paniculata (produced in Xishuangbanna, Yunnan Province) into a ground conical flask, add deionized water whose volume (ml) is 10 times the weight of Andrographis paniculata (g), soak for 30 minutes, and ultrasonically treat (power 300W , frequency 40kHz) for 30min, suction filtration, water recovery under reduced pressure, vacuum drying to obtain a dry extract, and calculate the yield. Wherein, the dry extract is the Andrographis paniculata sample.

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Abstract

The invention discloses a method for evaluating antimicrobial activity of andrographis paniculata. According to the method, the antimicrobial activity of andrographis paniculata is evaluated by antibacterial rate I%, wherein the I% is calculated according to the following formula (1):I%=(t2-t0) / t0*100%(1), t0 refers to the time to peak of a second exponential growth phase in a growth and metabolism thermogram of bacteria which is not subjected to pharmacological intervention, t2 refers to the time to peak of a second exponential growth phase in a growth and metabolism thermogram of bacteria which is subjected to intervention of andrographis paniculata, and the concentration of a test solution of the andrographis paniculata interfering growth and metabolism of the bacteria ranges from 0.4mg / mL to 4 mg / mL, preferably 3mg / mL. The method provided by the invention can be used for qualitatively analyzing the antimicrobial activity of andrographis paniculata produced in different production areas, different preparation methods, different production batches or different growth time, and the like.

Description

technical field [0001] The present application relates to a method for evaluating the antibacterial activity of traditional Chinese medicines, in particular to a method for evaluating the antibacterial activity of Andrographis paniculata. Background technique [0002] Andrographis paniculata (Burm.f.) Nees is the dry aerial part of the annual herbaceous plant Andrographis paniculata (Burm.f.) Nees. It is native to India, Sri Lanka, Thailand, Vietnam and other places. It was introduced and cultivated in my country in the 1950s. Guangxi, Hainan, Yunnan and other places have been introduced and cultivated in the warm regions north and south of the Yangtze River. Andrographis paniculata has the effects of clearing heat and detoxifying, cooling blood, and drying dampness. It is good at treating bacillary dysentery. [0003] At present, there are many methods for evaluating the antibacterial activity of drugs, such as fluorescence absorption method, dilution method, turbidimetric ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N25/20
Inventor 王伽伯王艳辉肖小河李硕房吉祥
Owner THE FIFTH MEDICAL CENT OF CHINESE PLA GENERAL HOSPITAL
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