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Application of ginsenoside Rb2 to protection of osteoblast precursor cell

A technology of osteogenic precursor cells and ginsenosides, applied in medical preparations containing active ingredients, bone diseases, organic active ingredients, etc., can solve problems such as damage to cell structure and function, reduce mineral deposition rate, and improve loose bone. Bone microstructure, improving the effect of oxidative stress

Inactive Publication Date: 2014-05-21
FOURTH MILITARY MEDICAL UNIVERSITY
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Problems solved by technology

However, high concentrations of ROS can lead to oxidation reactions of nucleic acids, proteins, and lipids, damage the structure and function of cells, and cause diseases

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  • Application of ginsenoside Rb2 to protection of osteoblast precursor cell
  • Application of ginsenoside Rb2 to protection of osteoblast precursor cell
  • Application of ginsenoside Rb2 to protection of osteoblast precursor cell

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Embodiment Construction

[0033] The present invention will be further described in detail below in conjunction with specific embodiments, which are explanations of the present invention rather than limitations.

[0034] MC3T3-E1 is an osteoblast precursor cell line that can differentiate into osteoblasts, and is a more commonly used cell model for studying the growth and differentiation of osteoblasts. H 2 o 2 Treatment is also a commonly used in vitro oxidative stress model. In order to observe the effect of ginsenoside Rb2 on apoptosis and differentiation of osteoblasts, and its anti-oxidative damage, MC3T3-E1 was selected as the cell model. H 2 o 2 Treated as an in vitro model of oxidative stress. As an animal model, the ovariectomized (castrated) mouse model is commonly used to simulate postmenopausal osteoporosis in women.

[0035] 1. Establishment of oxidative stress model in vitro

[0036] MC3T3-E1 cells were 2 × 10 3 Inoculate on 96-well plate, discard the medium after the cells are full,...

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Abstract

The invention discloses an application of ginsenoside Rb2 to protection of an osteoblast precursor cell. The ginsenoside Rb2 is non-toxic to the osteoblast precursor cell, does not influence cell proliferation, has a protecting effect on apoptosis of the osteoblast precursor cell (MC3T3-E1) caused by hydrogen peroxide (H2O2), can be used for improving the H2O2 induced oxidative stress status in the differentiation process, has a protecting effect on inhibition of MC3T3-E1 osteogenic differentiation caused by H2O2, and can inhibit expression of bone absorption factors, so that osteoporosis can be protected by improving osteoblast apoptosis and differential inhibition and indirectly inhibiting osteoclast activation. The ginsenoside Rb2 can improve the oxidative stress status in serum, improve the trabecular microstructure of the distal femur and the fourth lumbar vertebra, and reduce the mineral deposition rate of the distal femur, which indicates that the ginsenoside Rb2 can improve the bone microstructure by inhibiting oxidative stress to play the effect of preventing and treating osteoporosis.

Description

technical field [0001] The invention belongs to the technical field of prevention and treatment of osteoporosis, and relates to ginsenoside Rb 2 Application to protection of osteoblast precursor cells. Background technique [0002] Osteoporosis (Osteoporosis, OP) is a degenerative, systemic, and metabolic bone disease. It is caused by a variety of etiologies that result in loss of bone mass, structural changes, and decreased biomechanical properties of bone tissue. It is prone to fractures and poses a serious threat to the elderly. physical and mental health. [0003] Oxidative stress is a state in which highly active substances such as ROS (Reactive oxygen species) are produced excessively in the body, which exceeds the body's ability to remove them, resulting in an imbalance between oxidation and anti-oxidation. ROS in the physiological range play an important role in regulating normal functions of the human body, such as cell apoptosis, gene expression, and signal trans...

Claims

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Application Information

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IPC IPC(8): A61K31/704A61P19/10A61P19/08A61P39/06
Inventor 刘建黄强张金康杨柳罗卓荆
Owner FOURTH MILITARY MEDICAL UNIVERSITY
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