A kind of super selenium-enriched yeast and its application

Abstract

The invention relates to a super rich selenium yeast and the application thereof. The super rich selenium yeast is characterized in that the selenium-super rich yeast is selenium-super rich yeast separated from selenium mines in Enshi small reservoirs. The taxonomy name of the yeast is Wikerhamomyces anomala and the name is XS-1. On 15th, November, 2013, the yeast is preserved in the general microbiology center of China General Microbiological Culture Collection Center and the preservation number is CGMCC nO.8475. The super rich selenium yeast can be applied to the preparation for selenium-rich yeast.

Description

technical field

[0001] The invention relates to the technical field of microbial engineering, in particular to a super selenium-enriched yeast and its application. Background technique

[0002] Selenium is an indispensable trace element for animal growth. In animals, selenium is an essential component of glutathione peroxidase and thyroid 5'-deiodinase, and is also a component of many proteins in the body. It plays an important role in anti-oxidation, anti-stress and improving immunity.

[0003] More than 40 countries and regions in the world are selenium deficient areas. my country is a large selenium-deficient country. According to the "Atlas of Local Diseases and Environmental Factors of the People's Republic of China", there is a low-selenium zone in the area from the three northeastern provinces to the Yunnan-Guizhou Plateau, which accounts for 72% of my country's land area, of which 30% is In severe selenium deficient areas, the selenium content of natural foods such ...

Examples

Embodiment 1

[0030] (1) Activation of strains: the strain XS-1 of the present invention was inserted into the peptone yeast extract medium, activated on a plate for 24 hours, and the temperature was 28±1°C;

[0031] (2) Seed culture: After the activation of the strain XS-1, it was transferred to the wort medium and cultured in a shaker flask for 18 hours at a temperature of 28±1°C;

[0032] (3) Fermentation culture: prepare the fermentation medium, which includes the following components:

[0033]

[0034] Put the culture medium into the fermenter, stir evenly, and then sterilize under high pressure. After the sterilization is completed, inoculate yeast seeds into the fermenter for cultivation. The inoculation amount is 5%, the fermentation temperature is 28±2°C, pH 5.0, and the fermentation time is 48h.

[0035](4) Solid-liquid separation: The mixed liquid in the fermenter is centrifuged, washed, filtered and other steps to obtain yeast sludge.

[0036] (5) Detection and analysis: 5g ...

Embodiment 2

[0039] (1) Activation of strains: the strain XS-1 of the present invention was inserted into the peptone yeast extract medium, activated on a plate for 24 hours, and the temperature was 28±1°C;

[0040] (2) Seed culture: After the activation of the strain XS-1, it was transferred to the wort medium and cultured in a shaker flask for 18 hours at a temperature of 28±1°C;

[0041] (3) Fermentation culture: prepare the fermentation medium, which includes the following components:

[0042]

[0043] Put the culture medium into the fermenter, stir evenly, and then sterilize under high pressure. After the sterilization is completed, inoculate yeast seeds into the fermenter for cultivation. The inoculation amount is 8%, the fermentation temperature is 28±2°C, pH5.3, and the fermentation time is 72h.

[0044] (4) Solid-liquid separation: The mixed liquid in the fermenter is centrifuged, washed, filtered and other steps to obtain yeast sludge.

[0045] (5) Detection and analysis: 5g ...

Embodiment 3

[0048] (1) Activation of strains: the strain XS-1 of the present invention was inserted into the peptone yeast extract medium, activated on a plate for 24 hours, and the temperature was 28±1°C;

[0049] (2) Seed culture: After the activation of the strain XS-1, it was transferred to the wort medium and cultured in a shaker flask for 18 hours at a temperature of 28±1°C;

[0050] (3) Fermentation culture: prepare the fermentation medium, which includes the following components:

[0051]

[0052] Put the culture medium into the fermenter, stir evenly, and then sterilize under high pressure. After the sterilization is completed, inoculate yeast seeds into the fermenter for cultivation. The inoculation amount is 10%, the fermentation temperature is 28±2°C, pH5.4, and the fermentation time is 96h.

[0053] (4) Solid-liquid separation: The mixed liquid in the fermenter is centrifuged, washed, filtered and other steps to obtain yeast sludge.

[0054] (5) Detection and analysis: 5g...

technical field

[0001] The invention relates to the technical field of microbial engineering, in particular to a super selenium-enriched yeast and its application. Background technique

[0002] Selenium is an indispensable trace element for animal growth. In animals, selenium is an essential component of glutathione peroxidase and thyroid 5'-deiodinase, and is also a component of many proteins in the body. It plays an important role in anti-oxidation, anti-stress and improving immunity.

[0003] More than 40 countries and regions in the world are selenium deficient areas. my country is a large selenium-deficient country. According to the "Atlas of Local Diseases and Environmental Factors of the People's Republic of China", there is a low-selenium zone in the area from the three northeastern provinces to the Yunnan-Guizhou Plateau, which accounts for 72% of my country's land area, of which 30% is In severe selenium deficient areas, the selenium content of natural foods such ...

Examples

Embodiment 1

[0030] (1) Activation of strains: the strain XS-1 of the present invention was inserted into the peptone yeast extract medium, activated on a plate for 24 hours, and the temperature was 28±1°C;

[0031] (2) Seed culture: After the activation of the strain XS-1, it was transferred to the wort medium and cultured in a shaker flask for 18 hours at a temperature of 28±1°C;

[0032] (3) Fermentation culture: prepare the fermentation medium, which includes the following components:

[0033]

[0034] Put the culture medium into the fermenter, stir evenly, and then sterilize under high pressure. After the sterilization is completed, inoculate yeast seeds into the fermenter for cultivation. The inoculation amount is 5%, the fermentation temperature is 28±2°C, pH 5.0, and the fermentation time is 48h.

[0035](4) Solid-liquid separation: The mixed liquid in the fermenter is centrifuged, washed, filtered and other steps to obtain yeast sludge.

[0036] (5) Detection and analysis: 5g ...

Embodiment 2

[0039] (1) Activation of strains: the strain XS-1 of the present invention was inserted into the peptone yeast extract medium, activated on a plate for 24 hours, and the temperature was 28±1°C;

[0040] (2) Seed culture: After the activation of the strain XS-1, it was transferred to the wort medium and cultured in a shaker flask for 18 hours at a temperature of 28±1°C;

[0041] (3) Fermentation culture: prepare the fermentation medium, which includes the following components:

[0042]

[0043] Put the culture medium into the fermenter, stir evenly, and then sterilize under high pressure. After the sterilization is completed, inoculate yeast seeds into the fermenter for cultivation. The inoculation amount is 8%, the fermentation temperature is 28±2°C, pH5.3, and the fermentation time is 72h.

[0044] (4) Solid-liquid separation: The mixed liquid in the fermenter is centrifuged, washed, filtered and other steps to obtain yeast sludge.

[0045] (5) Detection and analysis: 5g ...

Embodiment 3

[0048] (1) Activation of strains: the strain XS-1 of the present invention was inserted into the peptone yeast extract medium, activated on a plate for 24 hours, and the temperature was 28±1°C;

[0049] (2) Seed culture: After the activation of the strain XS-1, it was transferred to the wort medium and cultured in a shaker flask for 18 hours at a temperature of 28±1°C;

[0050] (3) Fermentation culture: prepare the fermentation medium, which includes the following components:

[0051]

[0052] Put the culture medium into the fermenter, stir evenly, and then sterilize under high pressure. After the sterilization is completed, inoculate yeast seeds into the fermenter for cultivation. The inoculation amount is 10%, the fermentation temperature is 28±2°C, pH5.4, and the fermentation time is 96h.

[0053] (4) Solid-liquid separation: The mixed liquid in the fermenter is centrifuged, washed, filtered and other steps to obtain yeast sludge.

[0054] (5) Detection and analysis: 5g...

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