Lipoxygenase mutant with improved enzyme activity and thermal stability and preparation method

A technology of lipoxygenase and thermostability, which is applied in the field of enzyme engineering, can solve the problems of no lipoxygenase, etc., achieve the effects of improving enzyme activity and thermostability, high-efficiency expression, and solving low yield
CN103820404BInactive Publication Date: 2016-04-20NANJING AGRICULTURAL UNIVERSITY
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
NANJING AGRICULTURAL UNIVERSITY
Publication Date
2016-04-20
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a lipoxygenase mutant with improved enzyme activity and thermal stability and a preparation method thereof. The directional transformation of Anabaena lipoxygenase molecules is carried out by using directed evolution technology, and after multiple rounds of error-prone PCR and DNA ? Shuffling, obtained a lipoxygenase mutant with improved enzyme activity and thermostability at the same time, and the enzyme activity was 21420? U / mL is 2.17 times higher than the wild type, and its half-life t1 / 2 at 50°C is 1.9 times higher. Using this strategy can significantly improve the enzyme activity and thermal stability of lipoxygenase, solve the problems of low lipoxygenase activity and poor thermal stability, and provide a basis for its industrial production. The modified lipoxygenase is more suitable for industrial production and application.
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Description

Technical field

[0001] The invention involves enzyme activity and thermal stability at the same time, which is also increasing with fat oxygen enzymes and preparation methods. It belongs to the field of enzyme engineering technology. Background technique

[0002] Fatoxygenase, EC1.13.11.12, LOX) is widely existed in animal and plant, and it is found in algae, bread yeast, fungi, and cyanococcus.LOX can catalyze the polyunsaturated fatty acid oxidation of a polyunsaturated fatty acid in the dual-bonding of the dual-bonding of the dual-bonding dual-bond. These hydrogen oxides are lively.Chemical reaction intermediates have a wide range of application prospects in the fields of food, medicine, chemical industry, especially in food processing, such as improved quality of flour and its products, preparation of flavor compounds, tea processing, etc.Although people have already recognized the potential application value of LOX in food processing and LOX exists in nature, it is directly ...

Claims

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