16 results about "Anabaena sp." patented technology

The invention relates to the technical field of water treatment filter materials, in particular to an intelligent clean gradient composite water purification filter element and a preparation method thereof. The filter element adopts an integrated design of'natural gradient base material + ZIF-67 function enhancement + fluorescence-ultrasonic intelligent regeneration ': modified vegetable sponge is used as a porous support layer, a silica gel / ZIF-67 composite gradient layer is constructed on the porous support layer to realize adsorption and antibacterial dual functions, and dopamine-aluminum ions are used for composite surface modification to enhance hydrophilicity and interlayer binding force; a matched automatic system senses pollution in real time through the fluorescence monitoring module and triggers the ultrasonic regeneration module to complete self-cleaning, and the flux recovery rate is larger than or equal to 97.5%; the interception rate of the filtering element to anabaena under the operation pressure of 0.1-0.3 MPa is larger than or equal to 99.2%, the filtering flux is increased by 6-8 times compared with that of a commercial element, the filtering element can tolerate complex water quality in the coexistence of drugs and personal care products, natural vegetable sponge serves as a base material, and the filtering element has the advantages of biocompatibility and low cost and is suitable for drinking water purification and eutrophication water body treatment.

The invention discloses a phycomycete composite biological fertilizer for an arid loess area as well as a preparation method and application of the phycomycete composite biological fertilizer, and belongs to the technical field of biological fertilizers. The phycomycete composite biological fertilizer is prepared from nitrogen-fixing anabaena and acinetobacter, counted by a single cell, the total viable count of the anabaena azotica is greater than or equal to 1.0 * 10 < 6 > CFU / mL, and the total viable count of the acinetobacter is greater than or equal to 1.0 * 10 < 7 > CFU / mL. Experiments prove that the phycomycete composite biological fertilizer can significantly improve the germination potential and germination rate of crop seeds, promote seedling growth, improve the nutritional quality of crops and reduce the death rate of seedlings, is suitable for various crops such as wheat, Shanghai Qing and peas, and has important application value in agricultural production in arid regions.

The invention discloses a compound microbial agent and application thereof in prevention and control of cyanobacterial bloom, two algicidal bacteria are obtained through screening, the preservation number is CCTCC NO: M 2024698, and the preservation number is CCTCC NO: M 2024699, and the compound microbial agent is proved to have obvious inhibition effects on a plurality of cyanobacteria bacteria such as microcystis aeruginosa, lacustrine triphora, anabaena and cylindrocystis, and can be used for preventing and controlling cyanobacteria bloom. And the composite bacterial agent combined by the two algicidal bacteria has a remarkable algicidal effect on algal blooms in natural water areas, can be applied to the field of biological prevention and control of freshwater cyanobacterial blooms, and provides an ideal choice for treatment of cyanobacterial blooms in water bodies.

The invention belongs to the field of gene engineering, and particularly relates to an N-acetyl glucosamine isomerase mutant, a recombinant expression vector, a recombinant strain and application thereof. According to the invention, modification is carried out based on amino acid of wild type N-acetyl glucosamine isomerase from Anabaena sp.CH1, the modified mutant comprises two mutation sites, namely K84D and F349Y, and the activity is improved by 1.84 times. The invention further constructs a double-enzyme co-expression strain of N-acetylglucosamine isomerase and N-acetylneuraminic acid aldolase, and the strain is used for producing N-acetylneuraminic acid, so that the dosage of thalli is reduced, the conversion efficiency is improved, a more economical enzyme catalysis scheme is provided for industrial catalytic preparation of N-acetylneuraminic acid, and the method has a wide application prospect. The method has an important industrial application prospect.

The invention discloses a method for removing harmful algae. According to the method, silicate hollow microspheres are selected for flotation of the harmful algae such as microcystis and anabaena. In order to enhance the effect of removing harmful algae, aluminum salt containing Al < 3 + > is added into the water body environment before the flotation process. The method is simple in technological process, low in economic cost, suitable for removing harmful algae with different cell densities and high in removal rate under different pH conditions. The used silicate hollow microspheres have low density and high strength, the surfaces of the silicate hollow microspheres have certain roughness, combination with the two microalgae is facilitated, the method causes less pollution to the environment, and the floating beads can be repeatedly used for 3-4 times.

The invention discloses a method for site-specific modification of the cyclization efficiency of an anabaena ribozyme PIE carrier based on a cryoelectron microscope structure. The RNA (Ribonucleic Acid) of the Anabaena mutant comprises at least one of an RNA (Ribonucleic Acid) of an Anabaena intron G37 mutant and an RNA of an Anabaena PIE POLR2A mutant. The invention also provides an application of the mutant in research of increasing the yield of circRNA. The method has the beneficial effects that cryo-EM is utilized to analyze a tertiary structure of Anabaena ribozyme in a cyclization reaction process, a complete molecular structure mechanism for forming circRNA is researched through a cryoelectron microscope technology, the Anabaena mutant RNA with improved cyclization efficiency as well as the preparation method and application thereof are provided accordingly, the provided mutant RNA can increase the yield of circRNA by 1-2 times, and the yield of circRNA is increased by 1-2 times. The mutant is obviously superior to a wild type and other site mutants.

The invention provides a preparation method of a high-activity salmon salmon sperm polynucleotide PN (DNA sodium) extraction raw material and application of the high-activity salmon salmon sperm polynucleotide PN (DNA sodium) extraction raw material. Frozen salmon salmon sperm tissue, pretreated anabaena variabilis and fermented sake lactobacillus are used as ternary composite raw materials, mixed according to a specific proportion, subjected to impurity removal and then subjected to ultralow-temperature crushing, a composite buffer solution and composite salt are added, and the high-activity salmon salmon sperm polynucleotide PN (DNA sodium) extraction raw material is obtained. Performing ultrasonic-assisted stirring and low-temperature dispersion; then carrying out segmented enzymolysis at gradient pH and proper temperature by adopting a pH response type immobilized double-enzyme system, and automatically controlling the pH; the enzymatic hydrolysate is subjected to low-temperature multi-field coupling synergistic fragmentation to realize efficient release and homogenization of DNA, and then is subjected to low-temperature centrifugation, multistage membrane separation, specific capture and refining decoloration to finish graded purification; and finally adding a composite low-temperature protective agent and hyaluronic acid derivative microspheroidizing modification, and carrying out gradient heating and freeze-drying to obtain the product. The cyclic utilization rate of the enzymatic hydrolysate is greater than or equal to 75%, and the method is environment-friendly and efficient.

The invention discloses a biological cushion material based on microalgae, a preparation method of the biological cushion material and application of the biological cushion material in sandy soil improvement, and belongs to the technical field of agricultural ecological restoration. The preparation method of the biological cushion material comprises the following steps: (1) adding a microalgae suspension into a reaction kettle, adding glycerol according to the proportion, heating to 38-42 DEG C, stirring and dissolving, adding cationic starch, and stirring and mixing uniformly; and (2) adding polyurethane into the material prepared in the step (1), uniformly stirring, then adding sodium lignin sulfonate, reacting for 10-14 minutes at a constant temperature, and filtering the mixed liquid to obtain the biological cushion material. The microalgae is prepared from chlorella sorokiniana (Chlorella sorokiniana), Anabaena sp.) and Nodulcia cf.Harveyana (Nodulcia cf.Harveyana) according to a quantity ratio of 60: 20: 20, and the microalga is prepared from chlorella sorokiniana (Chlorella sorokiniana), Anabaena sp.) and Nodulcia cf.Harveyana (Nodulcia cf.Harveyana). The concentration of microalgae in the microalgae suspension is 1 * 10 < 6 >-1 * 10 < 7 > / mL; the addition amounts of the glycerol, the cationic starch, the sodium lignin sulfonate and the polyurethane are respectively 1 to 1.5 weight percent, 2 to 3 weight percent, 0.6 to 1 weight percent and 10 to 30 weight percent. The biological cushion material is wide in application range, and can be used as a soil conditioner, a coating agent of a slow / controlled-release fertilizer and a biological crust material for desertification control.

The invention discloses a microalgae composition and application thereof in fishing and farming integrated planting and breeding, and belongs to the technical field of aquaculture. The microalgae composition disclosed by the invention comprises chlorella, spirulina, nostoc, anabaena nitrogen fixation, schizochytrium limacinum and nannochloropsis oculata. The mass ratio of the chlorella to the spirulina to the nostoc to the nitrogen-fixing anabaena to the schizochytrium to the nannochloropsis oculata is 1: (4-5): (1: 1)-(2: 2.2-2.4): (3.4-3.6); the microalgae composition can be added into aquaculture feed to be applied to fishery and agricultural comprehensive planting and breeding; the fishing and farming integrated planting and breeding at least comprises rice-fish symbiosis; after the microalgae composition provided by the invention is applied to fishing and farming integrated planting, the growth of crops and aquaculture organisms for fishing and farming integrated planting can be effectively promoted.

The invention discloses algicidal bacteria TH41 and application thereof in inhibiting cyanobacterial bloom, and belongs to the technical field of biology. The algicidal bacterium TH41 is identified as bacillus pumilus TH41 and is preserved in the Guangdong Microbial Culture Collection Center, and the preservation number of the algicidal bacterium TH41 is GDMCC No: 66948. The invention also provides an algicidal bacterial agent containing the algicidal bacteria TH41. The invention further provides application of the algicidal bacterium TH41 or the algicidal fungicide in inhibiting cyanobacterial bloom. The algicidal bacterium TH41 has a degradation effect on harmful blue-green algae, has an efficient blue-green algae inhibition effect, has biological safety on daphnia magna and zebra fish in an algae killing concentration range, is suitable for preventing and treating various blue-green algae such as microcystis aeruginosa, cuscuta larakii, pichia mold and anabaena pseudosciaena, and has a wide application prospect.

The invention provides a multifunctional anabaena-bacterium compound growth-promoting organic fertilizer and a preparation method thereof. The organic fertilizer is prepared from the following raw materials in parts by weight: 60 to 80 parts of cow dung, 15 to 25 parts of modified porous attapulgite carrier, 0.5 to 1.5 parts of bacillus, 15 to 25 parts of anabaena powder, 8 to 12 parts of actinomycetes powder and 1 to 3 parts of trichoderma powder. According to the multifunctional anabaena-bacterium composite growth-promoting organic fertilizer disclosed by the invention, a continuous and synchronous fermentation process is carried out by adopting the modified porous attapulgite carrier loaded anabaena and beneficial bacteria, so that the biological function expression efficiency and the rhizosphere microorganism ecological regulation capability of the organic fertilizer are remarkably enhanced; the soil conditioner achieves an expected improvement effect in the aspects of soil reconstruction and agricultural green synergism, and can be widely applied to the fields of farmland soil pollution, soil quality improvement, atmospheric carbon and nitrogen fixation, crop yield increase and quality improvement and the like.

The present disclosure provides a variant of phenylalanine ammonia lyase (PAL), the phenylalanine ammonia lyase (PAL) being derived from Anabaena variabilis and comprises the amino acid sequence shown in SEQ ID NO: 1, wherein the variant comprises amino acid substitution C503S / C565L, so that the variant comprises the amino acid sequence shown in SEQ ID NO: 2; or amino acid substitution C503S / C565P, so that the variant comprises the amino acid sequence shown in SEQ ID NO: 3; or amino acid substitution C503L / C565P, so that the variant comprises the amino acid sequence shown in SEQ ID NO: 4. The present disclosure also provides a conjugate and a pharmaceutical composition comprising the variant, a polynucleotide encoding the variant, a bacterium comprising the variant, and a method for preparing the variant.

This invention discloses a modified attapulgite-based flocculant, its preparation method, and its application, belonging to the field of water body treatment technology for harmful algal blooms. The modified attapulgite-based flocculant of this invention comprises attapulgite powder and chitosan, with chitosan loaded on the surface of the attapulgite powder. The mass ratio of chitosan to attapulgite powder is 1:(2-14). This invention rationally utilizes attapulgite minerals based on the principle of waste-to-waste conversion; the preparation method is simple, non-toxic, and low-cost; through the modification method of this invention on different clay minerals, it was found that compared with other minerals studied in this invention (attapulgite, montmorillonite, pyrite, and bentonite), chitosan-modified attapulgite has excellent removal effects; the flocculant can effectively settle harmful algae in wastewater containing *Microcystis aeruginosa* or *Anabaena globulus*, and is suitable for various complex environments.

The invention belongs to the field of biological treatment of pollutants, and particularly relates to a method for jointly treating biogas slurry by using anabaena and trichoderma and application. The anabaena and the trichoderma viride are simultaneously cultured in the pig farm biogas slurry, so that ammonia nitrogen, phosphorus and COD in the pig farm biogas slurry can be effectively reduced. Meanwhile, the harvested compound of the anabaena and the trichoderma viride can be applied to rice fields and has application potential in the aspect of crop disease resistance.

The invention discloses a modified rectorite-based flocculant as well as a preparation method and application thereof, and belongs to the technical field of harmful algal bloom water treatment. The modified rectorite-based flocculant comprises rectorite powder and chitosan, the chitosan is loaded on the surface of the rectorite powder, and the mass ratio of the chitosan to the rectorite powder is 1: (2-14). According to the invention, rectorite minerals are reasonably utilized according to the principle of treating wastes with wastes; the preparation method is simple, non-toxic and low in cost; different clay minerals are modified through the modification method, and it is found that compared with other minerals (attapulgite, montmorillonite, pyrite and bentonite) researched in the invention, the chitosan modified rectorite has an excellent removal effect; when the flocculating agent is added into wastewater containing microcystis aeruginosa or anabaena cinerea, harmful algae can be effectively settled, and the flocculating agent is suitable for various complex environments.

The invention relates to the technical field of straw returning, and discloses a nitrogen-fixing cyanobacteria suspension for rice field straw returning and a preparation method and application thereof.The preparation method comprises the steps that nitrogen-fixing anabaena strains are subjected to activation-domestication culture and then subjected to gradient illumination amplification culture, and anabaena liquid is obtained; centrifuging the anabaena liquid, collecting precipitates, adding a stabilizer solution to adjust the concentration of algae cells, and then performing ultrasonic dispersion and stirring treatment to obtain the nitrogen-fixing cyanobacteria suspension. The application comprises the following steps: crushing the rice straws, and applying the complex microbial inoculant to perform ploughing operation; after rice seedlings are transplanted, the nitrogen-fixing cyanobacteria suspension for straw returning to the field is applied. By utilizing the efficient biological nitrogen fixation capacity of the nitrogen-fixing anabaena, after the suspension is prepared and applied to the field, a nitrogen source can be continuously provided for straw decomposition bacteria through the nitrogen fixation effect, the microbial decomposition requirement is met, nitrogen contention between the decomposition bacteria and rice plants is avoided, and sufficient nitrogen supply from the seedling establishment stage to the jointing stage of the rice is ensured.