The invention provides anabaena sp. PD-3 capable of efficiently degrading polychlorinated biphenyl (PCB), and application thereof to microbiological degradation of polychlorinated biphenyl. The anabaena sp. PD-3 is collected in the China Center For Type Culture Collection, with an address of Wuhan universality, Wuhan, China, a postal code of 430072, a collection date of September 8, 2013, and a collection number of CCTCC No.M2013398. The invention provides anabaena PD-3 capable of efficiently degrading polychlorinated biphenyl. The anabaena is capable of effectively degrading PCB28, 29, 30 and 31; after the exposure for a week, the degradation efficiency of the anabaena to PCB28 exceeds 60 percent, the dechlorinating percents of the anabaena to PCB44 and PCB61 are respectively 63.6 percent and 57.3 percent, and are superior to those (49.3 percent and 48.3 percent) of anabaena PD-1 (with a collection number of CCTCC No: M2011446). Thus, the anabaena PD-3 can be widely applied to the degradation of polychlorinated biphenyl in the environment, and has an important application prospect.