Needle mushroom-strain liquidation culture medium

A culture medium and Flammulina velutipes technology, which is applied in fertilizer mixtures, fertilization devices, applications, etc., can solve the problems of long growth cycle of solid strains, difficult transportation of liquid strains, and high investment cost for picking up impurities, and achieves good performance characteristics and neat mushrooms. The effect of good degree and less liquefaction residue

Inactive Publication Date: 2014-06-04
TIANSHUI ZHONGXING BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Solid strains have disadvantages such as long growth cycle, inconsistent bacterial age, and poor quality, while liquid strains have disadvantages such as difficult transportation, storage, testing, miscellaneous removal and high investment costs, which have become the main factors restricting the development of industrial edible fungi; using solid The liquefaction technology of Flammulina velutipes strains, which is the direct expansion of strains to liquid strains, solves the shortcomings of liquid strains that are not easy to transport, store, detect, pick up impurities and high investment costs. It also has the advantages of neat fruiting of liquid strains, fast growth time, The advantages of high yield, wherein the selection of liquefaction medium is a key technology, the solid strains cultivated by the medium can be directly expanded into liquid strains, which directly determines the success or failure of the liquefaction of Flammulina velutipes strains, and in the prior art There is no record of technology related to liquefaction of Flammulina velutipes strains

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0008] A kind of liquefaction culture medium of Flammulina velutipes strain, this culture medium is made up of raw material, water by the weight ratio of 1: 1.2, wherein the composition of said raw material is by weight percentage: paper pulp 45%, bagasse 44%, corn flour 6.6% , 1.1% each of sugar, gypsum, lime, and superphosphate.

[0009] Flammulina velutipes bacterial classification liquefaction preparation method comprises the steps: 1) test tube culture slant medium is PDA enrichment medium: potato 200g, corn flour 20g, bran 50g, glucose 20g, KH 2 PO 4 1g, MgSO 4 1g, VB 1 10 pieces, 20g of agar, 1000ml of water; insert the F8815 strain (Institute of Plant Protection, Chinese Academy of Agricultural Sciences) on the slope of the PDA enriched medium, and place it at 25°C for constant temperature cultivation for 8-10 days; 2) Cultivate the liquefied medium in a glass bottle Bottling after mixing and preparation, at 1.5kg / cm 3 Sterilize under pressure and 121°C for 1.5 ...

Embodiment 2

[0011] A liquefaction culture medium for Flammulina velutipes strains, the culture medium is composed of raw materials and water in a weight ratio of 1:1.3, wherein the components of the raw materials are by weight percentage: 50% of paper pulp, 40% of bagasse, and 6% of corn flour , 1% each of sugar, gypsum, lime, and superphosphate.

[0012] Flammulina velutipes bacterial classification liquefaction preparation method is the same as embodiment 1, repeats it no longer.

Embodiment 3

[0014] A kind of liquefaction culture medium of Flammulina velutipes strain, this culture medium is made up of raw material, water by the weight ratio of 1:1.25, wherein the composition of said raw material is by weight percentage: pulp 55%, bagasse 36%, corn flour 5.4% , 0.9% each of sugar, gypsum, lime, and superphosphate.

[0015] Flammulina velutipes bacterial classification liquefaction preparation method is the same as embodiment 1, repeats it no longer.

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PUM

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Abstract

The invention discloses a needle mushroom-strain liquidation culture medium. A solid strain cultured by utilizing the culture medium can be directly made into a liquid strain. The culture medium is prepared from raw materials and water according to a weight ratio of 1:(1.2-1.3), wherein the raw materials include the following components in percentage by weight: 45-55% of paper pulp, 36-44% of bagasse, 5.4-6.6% of corn flour, 0.9-1.1% of sugar, 0.9-1.1% of plaster, 0.9-1.1% lime of and 0.9-1.1% of calcium superphosphate.

Description

technical field [0001] The invention relates to a culture medium of Flammulina velutipes, in particular to a liquefaction culture medium of Flammulina velutipes. Background technique [0002] Solid strains have disadvantages such as long growth cycle, inconsistent bacterial age, and poor quality, while liquid strains have disadvantages such as difficult transportation, storage, testing, miscellaneous removal and high investment costs, which have become the main factors restricting the development of industrial edible fungi; using solid The liquefaction technology of Flammulina velutipes strains, which is the direct expansion of strains to liquid strains, solves the shortcomings of liquid strains that are not easy to transport, store, detect, pick up impurities and high investment costs. It also has the advantages of neat fruiting of liquid strains, fast growth time, The advantages of high yield, wherein the selection of liquefaction medium is a key technology, the solid stra...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05G1/00
Inventor 陶军
Owner TIANSHUI ZHONGXING BIO TECH
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