A kind of preparation method and application of low ground tea polysaccharide

A technology of low ground tea and polysaccharides, which is applied in the field of medicine, can solve the problems that the research on low ground tea polysaccharides has not been reported, and achieve the effects of rich raw materials, improved immunity, good social and economic benefits

Active Publication Date: 2016-04-27
HENAN UNIV OF CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The current research on its active ingredients mainly involves coumarins, flavonoids and polyphenols, triterpenoids, volatile oils, etc., but the research on polysaccharides of dwarf tea has not been reported yet

Method used

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  • A kind of preparation method and application of low ground tea polysaccharide
  • A kind of preparation method and application of low ground tea polysaccharide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] The preparation method of the short tea polysaccharide of the present embodiment comprises the following steps:

[0015] (1) Take the Aodi tea and dry it, crush it into powder; weigh the Aodi tea powder, add distilled water to the Aodi tea powder, the weight ratio of the Aodi tea powder to the distilled water is 1:10, soak for 30min, at 450W power Ultrasonic for 50 minutes, then heated at 70°C for 60s at the same power, and then heated at 70°C for 60s at an interval of 120s, and then at an interval of 120s. Ethanol to make the ethanol content reach the volume concentration of 80%, let it stand at 4°C for 12 hours, centrifuge to remove the supernatant, collect the precipitate, wash off the water in the precipitate with absolute ethanol, and then wash away the absolute ethanol in the precipitate with acetone , and finally wash off the acetone in the precipitate with anhydrous ether, and dry under reduced pressure to obtain crude tea polysaccharide;

[0016] (2) Add the c...

Embodiment 2

[0018] The preparation method of the short tea polysaccharide of the present embodiment comprises the following steps:

[0019] (1) Take the Aodi tea and dry it, crush it into powder; weigh the Aodi tea powder, add distilled water to the Aodi tea powder, the weight ratio of the Aodi tea powder to the distilled water is 1:15, soak for 35min, at a power of 300W Ultrasound for 30 minutes, then heated at 80°C for 55s at the same power, followed by heating at 80°C for 55s at an interval of 130s, and then at an interval of 130s. Ethanol to make the ethanol content reach the volume concentration of 60%, let stand at 5°C for 16 hours, centrifuge to remove the supernatant, collect the precipitate, wash away the moisture in the precipitate with absolute ethanol, and then wash away the absolute ethanol in the precipitate with acetone , and finally wash off the acetone in the precipitate with anhydrous ether, and dry under reduced pressure to obtain crude tea polysaccharide;

[0020] (2)...

Embodiment 3

[0022] The preparation method of the short tea polysaccharide of the present embodiment comprises the following steps:

[0023] (1) Take the Aodi tea and dry it, crush it into powder; weigh the Aodi tea powder, add distilled water to the Aodi tea powder, the weight ratio of the Aodi tea powder to the distilled water is 1:20, soak for 25min, at 600W power Ultrasonic for 90 minutes, then heated at 75°C for 65s at the same power, and then heated at 75°C for 65s at an interval of 110s, and then at an interval of 110s. Ethanol to make the ethanol content reach the volume concentration of 70%, let it stand at 6°C for 14 hours, centrifuge to remove the supernatant, collect the precipitate, wash away the moisture in the precipitate with absolute ethanol, and then wash away the absolute ethanol in the precipitate with acetone , and finally wash off the acetone in the precipitate with anhydrous ether, and dry under reduced pressure to obtain crude tea polysaccharide;

[0024] (2) Add t...

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Abstract

The invention discloses a preparation method and application of herba ardisiae japonicae polysaccharide, and is used for effectively solving the problems of preparing the herba ardisiae japonicae polysaccharide and effectively applying the herba ardisiae japonicae polysaccharide to an anti-fatigue and immunity-improving drug. A technical scheme for solving the problems is as follows: adding distilled water into the dried herba ardisiae japonicae powder for soaking, carrying out ultrasonic treatment and microwave intermittent heating, filtering to obtain filtrate, concentrating the filtrate and adding alcohol, centrifuging and washing precipitates to obtain a herba ardisiae japonicae polysaccharide crude product; purifying the crude product to obtain the herba ardisiae japonicae polysaccharide. The herba ardisiae japonicae polysaccharide prepared by the preparation method disclosed by the invention takes natural traditional Chinese medicine materials as raw materials, so that the produced herba ardisiae japonicae polysaccharide is ensured to be free of toxic and side effect, capable of effectively resisting fatigue and improving immunity of a body by being applied to a drug, has good social benefits and economic benefits, and is innovation on traditional Chinese medicines.

Description

technical field [0001] The invention relates to the field of medicine, in particular to a preparation method and application of tea polysaccharide. Background technique [0002] Low ground tea is the dry whole herb of Ardisiajaponica (Thunb.) Blume, also known as flat ground wood, don't grow old, not out of the forest, and leaf bottom pearl. It has the effects of relieving cough and reducing phlegm, removing blood stasis and detoxification, diuresis, and relieving pain; it is mainly used for sore throat, joint pain, amenorrhea, tuberculosis, chronic bronchitis, jaundice hepatitis, and fatigue. The current research on its active ingredients mainly involves coumarins, flavonoids and polyphenols, triterpenoids, volatile oils, etc., but the research on the polysaccharides of Dwarf Tea has not been reported yet. Polysaccharides are polymers in which aldose and / or ketose are linked together by glycosidic bonds in nature. It is an essential component of all living organisms and is...

Claims

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Application Information

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Patent Type & AuthorityPatents(China)
IPC IPC(8): C08B37/00A61P39/00A61P37/04
Inventor李建生刘富岗冯素香吴兆宇王蒙蒙李晓玉
OwnerHENAN UNIV OF CHINESE MEDICINE