Aaptamine alkaloid compound in sponge from Paracel Islands and anti-tumor application of alkaloid compound
A technology of alkaloids and compounds, applied in antitumor drugs, drug combinations, organic chemistry, etc., to achieve significant antitumor activity and simple preparation methods
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Embodiment 1
[0028] Embodiment 1. Preparation of compounds of the present invention
[0029] 1. Prepare dichloromethane crude extract
[0030] Take 2.3 kg of dry and pulverized sponge sponge, use 15 L of 95% ethanol to percolate and extract 4 times, each time for 3 days, combine the extracts, concentrate the extracts under reduced pressure to obtain extracts, and extract with dichloromethane solution to obtain dichloromethane Methylene chloride total extract, suspend the total dichloromethane extract with 90% methanol, degrease with n-hexane, add water to adjust the methanol concentration of the suspension to 60%, extract with dichloromethane 3 times, concentrate the extract Obtain 84.4g of dichloromethane crude extract;
[0031] 2. Separation and purification
[0032] 1) Separation and purification: the crude dichloromethane extract was subjected to VLC decompression column chromatography, and methanol:dichloromethane=100:1, 80:1, 60:1, 50:1, 30:1, 20:1, 10:1, 8:1, 5:1, 1:1, 0:1 were u...
Embodiment 2
[0041] Embodiment 2 The in vitro antitumor activity experiment of the compound of the present invention:
[0042] For compound C of the present invention 14 h 13 N 3 o 3 In vitro anti-tumor experiments were carried out, and the tumor cell lines used were as follows:
[0043] Human acute leukemia cell HL60 (HL60), purchased from ATCC, ATCC number is CCL-240;
[0044] Human chronic leukemia cell K562 (K562), purchased from ATCC, ATCC number is CCL-243;
[0045] Human breast cancer cell MCF-7 (MCF-7), purchased from ATCC, ATCC number is HTB-22;
[0046] Human nasopharyngeal carcinoma cell line KB (KB), purchased from ATCC, ATCC number is CCL-17;
[0047] Human liver cancer Hep G2 (Hep G2), purchased from ATCC, ATCC number is HB-8065;
[0048] Human colon cancer cell line HT-29 (HT-29), purchased from ATCC, ATCC number is HTB-38.
[0049] Human acute leukemia cells HL60 and human chronic leukemia cells K562, both cell lines were grown in suspension in RPMI-1640 medium with...
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