The invention relates to a method for verifying and fermenting polar marine
ray fungi AFN1007 producing antitumor active substance, and the research on the
antitumor activity of the zymotic fluid thereof, belonging to the microbial
medicine field. The strain AFN1007 is verified to be a novel kind of
Streptomyces by the traditional classification and the analysis of 16S rDNA
gene of the AFN1007. The compositions of a culture medium by percentage are: 1 percent of soybean flour, 1 percent of tragantine, 0.1 percent of KNO3, 0.05 percent of MgSO4, 0.001 percent of FeSO4, 0.05 percent of NaCl and natural
seawater with a pH value between 7.2 and 7.4. The strain is fermented by using the culture medium. The
MTT method shows that the zymotic fluid has strong antitumor action on four
tumor cells of KB, BGC 803, Hep G2 and
Hela and an antitumor compound with a novel structure is expected to be found in the zymotic fluid.