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Saponin compound extracted from Momordica grosvenori root, preparation and use thereof

A technology of saponins and compounds, which is applied in the field of preparation of saponins and the above-mentioned saponins, and can solve problems such as no reports of water-soluble saponins

Inactive Publication Date: 2009-05-27
GUANGXI INST OF BOTANY THE CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] So far, there have only been reports on the chemical components of the fat-soluble part of Luo Han Guo root, but no reports on its water-soluble saponins

Method used

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  • Saponin compound extracted from Momordica grosvenori root, preparation and use thereof
  • Saponin compound extracted from Momordica grosvenori root, preparation and use thereof
  • Saponin compound extracted from Momordica grosvenori root, preparation and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Get 3,000 grams of dried Luo Han Guo root fragments (30 mesh) collected in Longjiang Township, Yongfu, Guilin, Guangxi, soak them in 15 liters of methanol with a volume concentration of 80%, extract them under reflux at 80°C for 3 times, each time for 3 hours, filter, and combine the extractions liquid, and recover methanol under reduced pressure to obtain 120 grams of extract. The extract was dissolved in 2 liters of distilled water, filtered, and the filtrate was passed through a D101 type macroporous resin column, followed by water, methanol with a volume concentration of 50%, and methanol with a volume concentration of 100% for gradient elution, and the 100% methanol eluate was collected. Methanol was recovered under reduced pressure to obtain 5.1 g of extract, which was then chromatographed on a silica gel column and eluted with a gradient of a mixed solvent of chloroform:methanol:water=20:1:0.1~5:1:0.1 (v / v). Analysis and detection, collecting the fractions contai...

Embodiment 2

[0022] Get 2000 grams of dried Luo Han Guo root fragments (20 mesh) collected in Longjiang Township, Yongfu, Guilin, Guangxi, soak them in 8 liters of methanol with a volume concentration of 80%, reflux extraction at 80 ° C for 3 times, each time for 2 hours, filter, and combine the extractions Liquid, decompression recovery ethanol to obtain 78 grams of extract. The extract was dissolved in 1 liter of distilled water, filtered, and the filtrate was passed through an AB-8 type macroporous resin column, followed by water, methanol with a volume concentration of 50%, and methanol with a volume concentration of 100% for gradient elution, and collected for elution with 100% methanol liquid, methanol was recovered under reduced pressure to obtain 3.5 grams of extract, and then silica gel column chromatography was used for gradient elution with a mixed solvent of chloroform:methanol:water=20:1:0.1~5:1:0.1 (v / v). For layer chromatography detection, the fractions containing Siraitic a...

Embodiment 3

[0024] Get 3000 grams of dried Luo Han Guo root fragments (40 mesh) collected in Longjiang Township, Yongfu, Guilin, Guangxi, soak them in 18 liters of ethanol with a volume concentration of 90%, extract twice at 85° C. for 4 hours each time, filter, and combine the extracts , Ethanol was recovered under reduced pressure to obtain 130 grams of extract. The extract was dissolved in 2 liters of distilled water, filtered, and the filtrate was passed through a Diaion HP20 type macroporous resin column, followed by water, methanol with a volume concentration of 50%, and methanol with a volume concentration of 100% for gradient elution, and the 100% methanol eluate was collected , reclaim methanol under reduced pressure to obtain 5.7 grams of extract, then use silica gel column chromatography, elution with chloroform:methanol:water=20:1:0.1~5:1:0.1 (v / v) mixed solvent gradient, thin layer For chromatographic detection, the fractions containing Siraitic acid IIB and Siraitic acid IIC...

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Abstract

The invention relates to the medicine technical field, in particular to saponin compounds of Siraitic acid IIB and Siraitic acid IIC which are extracted and separated from fructus momordicae roots, a method for preparing the same and application thereof in an anti-tumor medicine. The molecular formulas of the two compounds are C40H60O14 and C41H62O15 respectively. By various modern spectral analysis, particularly the application of advanced two-dimensional nuclear magnetic resonance spectrum to comprehensive analysis, the chemical structures of the two compounds are determined. In vitro anti-tumor tests show that the compound Siraitic acid IIB can obviously inhibit lung cancer cells A-549 and liver cancer cells Hep-G2, and the compound Siraitic acid IIC can also obviously inhibit liver cancer cells Hep-G2. The invention provides lead compound for research on a new anti-tumor medicine, and has essential values in comprehensively developing and utilizing fructus momordicae resources.

Description

(1) Technical field: [0001] The invention relates to the technical field of medicine, in particular to a saponin compound with a novel structure isolated from the root of Luo Han Guo; the invention also relates to a preparation method and application of the saponin compound. (two) background technology: [0002] Luo Han Guo Siraitia grosvenrii (Swingle) C. Jeffrey is a perennial rhizome plant of Cucurbitaceae Luo Han Guo. It is a unique economic and medicinal plant in my country and a traditional specialty in northern Guangxi. Luo Han Guo has a well-developed root system, hypertrophic root tubers, bitter taste, and slightly cold nature. It has the effects of clearing away heat and dampness, dredging collaterals and relieving pain. Pharmacological experiments have shown that the extract of Luo Han Guo root has various effects such as anti-inflammatory, analgesic, decomposing stems, and reducing enzymes. Clinical trials have proved that it has a significant effect on rheumatoi...

Claims

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Application Information

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IPC IPC(8): C07J71/00C07J17/00A61K31/704A61K31/7048A61P35/00
Inventor 李典鹏刘金磊卢艺芳卢风来黄永林陈月圆文永新野原稔弘
Owner GUANGXI INST OF BOTANY THE CHINESE ACAD OF SCI
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