Molecular cloning and application of glp2r gene fragment related to pork quality traits
A technology of gene fragments and traits, applied in the field of single nucleotide polymorphism sites, including nucleic acid molecules of porcine gene nucleotides as shown in SEQ ID NO: 1, to achieve the effect of improving production economic benefits
Inactive Publication Date: 2016-02-24
HUNAN AGRICULTURAL UNIV
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Problems solved by technology
At present, GLP2R has been cloned and expressed in rats, humans, and mice, but there are few reports on pig GLP2R gene research at home and abroad
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Embodiment Construction
[0014] The present invention will be further explained below in conjunction with specific examples, but the specific implementation does not limit the present invention in any way.
[0015] Cloning of the GLP2R gene:
[0016] Using the human GLP2R gene sequence (GenBank accession number NM_004246.1) as the seed sequence, using BLASTN in GenBank to design specific primers with reference to the porcine EST (Expressed Sequence Tags) with more than 80% homology, and using RACE technology to clone the porcine Full-length cDNA of GLP2R gene.
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The invention belongs to the technical field of livestock molecular biology and particularly relates to a molecular cloning of GLP2R gene fragments associated with pork quality traits and application of GLP2R gene fragments. The human GLP2R gene sequence is taken as a seed sequence, specific primers are designed by BLASTN in GenBank and referring to swine EST of which the homology is above 80% and swine GLP2R gene cDNA full-length is cloned by RACE. DNA sequence of the swine GLP2R gene fragments is shown in SEQ ID NO:1. GLP2R gene polymorphism is determined by designing a primer based on human GLP2R gene via comparative genomics method, amplifying by swine genomic DNA as template, screening SNP from amplification fragment via sequencing, and genotyping by PCR-RFLP. A / G base mutation resulting in PCR-RFLP-BstEII polymorphism is found at 244bp, a new molecular marker is provided for swine marker-assisted selection and the situation of pig breeds at home and abroad can be detected by the marker.
Description
technical field [0001] The invention belongs to the technical field of livestock molecular biology, and in particular relates to a nucleic acid molecule comprising porcine gene nucleotides as shown in SEQ ID NO:1. The present invention also relates to the molecular cloning method of the GLP2R gene shown in SEQ ID NO: 1, the single nucleotide polymorphism site in the polynucleotide sequence and the method for detecting the single nucleotide polymorphism site. Background technique [0002] Pork is the main source of animal protein. As people's demand for meat increases, the requirements for meat quality also increase accordingly. There are genetic and non-genetic factors that affect pork quality. The fat content in pig muscle has a great influence on the meat quality. Generally speaking, the higher the fat content in the muscle, the tenderer the pork quality, and the better the meat quality. Most meat quality traits belong to quantitative traits, which are controlled by minor...
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IPC IPC(8): C12N15/12C12Q1/68C12N15/11
Inventor 马海明柴玉兰王玲玉杨虎
Owner HUNAN AGRICULTURAL UNIV
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