A construction method of ecological canal
A construction method and water channel technology, applied in the fields of botanical equipment and methods, horticulture, coastline protection, etc., can solve the problems of low degree of development and difficulty in further maintaining the stability of canal slope protection, and achieve the effect of increasing stability
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[0021] Among them, as a particularly preferred embodiment of the present invention, the solid medium contains 400-700g / kg of straw, 100-300g / kg of bean cake powder, 2-10g / kg of potassium dihydrogen phosphate and 1-10g of / kg of ferrous sulfate. Wherein, the solid medium may also contain the rest of water and / or other components. In this preferred mode, on the one hand, Bacillus subtilis can grow rapidly during the fermentation process, and on the other hand, the obtained fermented material can be used as a microbial agent, which can better colonize and establish a microenvironment in the slope protection soil of the canal.
[0022] Wherein, the straw can be crushed into a conventional size that can be used for fermentation, for example, the length can be 5-10mm, the width can be 3-8mm, and the thickness can be 3-8mm. The straw may be straw from various sources, for example including at least one of corn straw, sorghum straw, rice straw, wheat straw and soybean straw.
[0023...
preparation Embodiment 1
[0032] This preparation example is used to illustrate the cultivation of Bacillus subtilis and the preparation of microbial inoculum of the present invention.
[0033] Inoculate Bacillus subtilis (Bacillus subtilis) with the preservation number CCTCCNO: M2013498 into LB medium (containing 10 g / L of tryptone, 5 g / L of yeast extract and 10 g / L of NaCl), at 35 ° C and 80 rpm Cultivate under shaking per minute until the concentration of viable bacteria is 10 10 CFU / mL to obtain the seed liquid of Bacillus subtilis.
[0034] Inoculate Bacillus licheniformis (Bacillus licheniformis) with the preservation number CCTCCNO: M2013497 into LB medium (containing 10 g / L of tryptone, 5 g / L of yeast extract and 10 g / L of NaCl), at 35 ° C and 80 rpm Cultivate under shaking per minute until the concentration of viable bacteria is 5×10 10 CFU / mL to obtain the seed bacterial liquid of Bacillus licheniformis.
[0035] The seed bacterium liquid of 100mL above-mentioned Bacillus subtilis and the ...
preparation Embodiment 2
[0037] This preparation example is used to illustrate the cultivation of Bacillus subtilis and the preparation of microbial inoculum of the present invention.
[0038] Inoculate Bacillus subtilis (Bacillus subtilis) with the preservation number CCTCCNO: M2013498 into LB medium (containing 10 g / L of tryptone, 5 g / L of yeast extract and 10 g / L of NaCl), at 35 ° C and 80 rpm Cultivate under shaking per minute until the concentration of viable bacteria is 10 10 CFU / mL to obtain the seed liquid of Bacillus subtilis.
[0039] Inoculate Bacillus licheniformis (Bacillus licheniformis) with the preservation number CCTCCNO: M2013497 into LB medium (containing 10 g / L of tryptone, 5 g / L of yeast extract and 10 g / L of NaCl), at 35 ° C and 80 rpm Cultivate under shaking per minute until the concentration of live bacteria is 10×10 10 CFU / mL to obtain the seed bacterial liquid of Bacillus licheniformis.
[0040] The seed bacterium liquid of 100mL above-mentioned Bacillus subtilis and the s...
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