A method for preparing high-purity tea saponin by using enzyme method combined with membrane technology

Abstract

The invention relates to a method for preparing high-purity tea saponin by using an enzymic method together with a membrane technique. The method is characterized by comprising the following steps: a, crushing tea seed meal by using a ultrafine grinding technique; b, adding water for enzymolysis into a substance in the step a; c, centrifuging the enzymatic hydrolysate obtained in the step b; d, passing the supernate obtained in the step c through an ultrafiltration membrane to obtain crude tea saponin; e, re-adjusting a concentrated solution obtained in the step b to be of weak alkalescence, and further performing concentration treatment by using an ultrafiltration membrane 2, and intermittently adding pure water for dilution in the filtration process; and f, performing spray drying on the concentrated solution obtained in the sep e, thereby obtaining a product. By adopting the method, the process is simple, the scheme is efficient, the product is safe, the tea seed meal resource can be more efficiently utilized, the problems that a conventional process is low in yield, the purity is insufficient and the environment is polluted are solved, and a source of safe and degradable natural surfactant is provided for environment protection.

Description

technical field

[0001] The invention relates to a method for preparing high-purity tea saponin by using enzyme method combined with membrane technology. Background technique

[0002] Since ancient times, China has a tradition of using tea seed cakes after tea seed oil extraction for daily personal hygiene and cleaning. Modern studies have shown that tea saponin is the core component of cleaning and decontamination. However, due to the high cost of high-purity saponin caused by the scarcity of Camellia oleifera resources and the immature production process, tea saponin has become a major player in the surfactant market. performance is not active. This technology aims to develop a production process of low-cost high-purity tea saponin, and improve the competitiveness of high-purity tea saponin in the surfactant market, especially the daily surfactant market.

[0003] Tea seed meal is a by-product called tea seed meal that is separated by pressing after camellia oleifera is p...

Examples

Embodiment 1

[0040] Embodiment 1: a kind of method utilizing biological method combined with membrane technology to extract tea saponin is to prepare the method for high-purity tea saponin to carry out as follows:

[0041] a. Using ultra-fine crushing technology to crush the tea seed meal;

[0042] b, hydrolyzing the substance in step a with water;

[0043] c. centrifuging the enzymatic hydrolyzate obtained in step b;

[0044] d, the supernatant obtained in the c step is passed through an ultrafiltration membrane to obtain crude saponin;

[0045] e, readjust the concentrated solution in step d to be slightly alkaline, use the ultrafiltration membrane 2 to carry out further concentration treatment, and add pure water intermittently to dilute during the filtration process;

[0046] f. Spray-dry the concentrate in step e to obtain the product.

[0047] In the step a, the tea seed meal is pulverized to 100-120 mesh.

[0048] In the b step, add water accounting for 8 times the weight of the...

Embodiment 2

[0056] Embodiment 2: a kind of method utilizing biological method combined membrane technology to extract tea saponin is to prepare the method for high-purity tea saponin to carry out as follows:

[0057] a. Using ultra-fine crushing technology to crush the tea seed meal;

[0058] b, hydrolyzing the substance in step a with water;

[0059] c. centrifuging the enzymatic hydrolyzate obtained in step b;

[0060] d, the supernatant obtained in the c step is passed through an ultrafiltration membrane to obtain crude saponin;

[0061] e, readjust the concentrated solution in step d to be slightly alkaline, use the ultrafiltration membrane 2 to carry out further concentration treatment, and add pure water intermittently to dilute during the filtration process;

[0062] f. Spray-dry the concentrate in step e to obtain the product.

[0063] In the step a, the tea seed meal is pulverized to 100-120 mesh.

[0064] In the b step, add water accounting for 8 times the weight of the tea ...

Embodiment 3

[0072] Embodiment 3: a kind of method utilizing biological method combined membrane technology to extract tea saponin, is to prepare the method for high-purity tea saponin to carry out as follows:

[0073] a. The tea seed meal is pulverized by ultra-fine pulverization technology; the tea seed meal is pulverized to 100-120 mesh.

[0074] b. hydrolyzing the substance in step a with water; the enzyme is an industrially produced protease. The protease produced industrially is papain. Add 8 times the weight of tea seed cake water, control the temperature at 10°C, add 0.5% of the tea seed kernel weight of enzyme under the condition of pH 8.5, and enzymatically hydrolyze for 4 hours;

[0075] c. Centrifuge the enzymatic hydrolyzate obtained in step b; adjust the pH of the centrifuge to 6-7 and then centrifuge;

[0076] d. Pass the supernatant obtained in the c step through an ultrafiltration membrane to obtain a crude saponin solution; adjust the pH to 2-3; The conditions are: pre...

technical field

[0001] The invention relates to a method for preparing high-purity tea saponin by using enzyme method combined with membrane technology. Background technique

[0002] Since ancient times, China has a tradition of using tea seed cakes after tea seed oil extraction for daily personal hygiene and cleaning. Modern studies have shown that tea saponin is the core component of cleaning and decontamination. However, due to the high cost of high-purity saponin caused by the scarcity of Camellia oleifera resources and the immature production process, tea saponin has become a major player in the surfactant market. performance is not active. This technology aims to develop a production process of low-cost high-purity tea saponin, and improve the competitiveness of high-purity tea saponin in the surfactant market, especially the daily surfactant market.

[0003] Tea seed meal is a by-product called tea seed meal that is separated by pressing after camellia oleifera is p...

Examples

Embodiment 1

[0040] Embodiment 1: a kind of method utilizing biological method combined with membrane technology to extract tea saponin is to prepare the method for high-purity tea saponin to carry out as follows:

[0041] a. Using ultra-fine crushing technology to crush the tea seed meal;

[0042] b, hydrolyzing the substance in step a with water;

[0043] c. centrifuging the enzymatic hydrolyzate obtained in step b;

[0044] d, the supernatant obtained in the c step is passed through an ultrafiltration membrane to obtain crude saponin;

[0045] e, readjust the concentrated solution in step d to be slightly alkaline, use the ultrafiltration membrane 2 to carry out further concentration treatment, and add pure water intermittently to dilute during the filtration process;

[0046] f. Spray-dry the concentrate in step e to obtain the product.

[0047] In the step a, the tea seed meal is pulverized to 100-120 mesh.

[0048] In the b step, add water accounting for 8 times the weight of the...

Embodiment 2

[0056] Embodiment 2: a kind of method utilizing biological method combined membrane technology to extract tea saponin is to prepare the method for high-purity tea saponin to carry out as follows:

[0057] a. Using ultra-fine crushing technology to crush the tea seed meal;

[0058] b, hydrolyzing the substance in step a with water;

[0059] c. centrifuging the enzymatic hydrolyzate obtained in step b;

[0060] d, the supernatant obtained in the c step is passed through an ultrafiltration membrane to obtain crude saponin;

[0061] e, readjust the concentrated solution in step d to be slightly alkaline, use the ultrafiltration membrane 2 to carry out further concentration treatment, and add pure water intermittently to dilute during the filtration process;

[0062] f. Spray-dry the concentrate in step e to obtain the product.

[0063] In the step a, the tea seed meal is pulverized to 100-120 mesh.

[0064] In the b step, add water accounting for 8 times the weight of the tea ...

Embodiment 3

[0072] Embodiment 3: a kind of method utilizing biological method combined membrane technology to extract tea saponin, is to prepare the method for high-purity tea saponin to carry out as follows:

[0073] a. The tea seed meal is pulverized by ultra-fine pulverization technology; the tea seed meal is pulverized to 100-120 mesh.

[0074] b. hydrolyzing the substance in step a with water; the enzyme is an industrially produced protease. The protease produced industrially is papain. Add 8 times the weight of tea seed cake water, control the temperature at 10°C, add 0.5% of the tea seed kernel weight of enzyme under the condition of pH 8.5, and enzymatically hydrolyze for 4 hours;

[0075] c. Centrifuge the enzymatic hydrolyzate obtained in step b; adjust the pH of the centrifuge to 6-7 and then centrifuge;

[0076] d. Pass the supernatant obtained in the c step through an ultrafiltration membrane to obtain a crude saponin solution; adjust the pH to 2-3; The conditions are: pre...