Micro-fluidic chip for screening hepatoenteral circulation drugs

A technology of microfluidic chips and enterohepatic circulation, which is applied to laboratory containers, laboratory utensils, biochemical equipment and methods, etc., can solve time-consuming, labor-intensive, money-consuming, moral and ethical issues

Inactive Publication Date: 2014-08-13
DALIAN UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to overcome the time-consuming, labor-intensive, money-consuming and ethical issues involved in the existing methods of screening drugs for enterohepatic circulation, the present invention provides a microfluidic chip that can be used for screening drugs for enterohepatic circulation

Method used

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  • Micro-fluidic chip for screening hepatoenteral circulation drugs
  • Micro-fluidic chip for screening hepatoenteral circulation drugs
  • Micro-fluidic chip for screening hepatoenteral circulation drugs

Examples

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Embodiment

[0024] (1) Choose optical glass as the substrate of the microfluidic chip, place the substrate in a glass jar filled with H2O-NH4OH-H2O2 (5:1:1), and wash the substrate with a 75°C water bath until its surface Bubbles appear again; wash with deionized water, and ultrasonicate three times in a 50°C water bath for 25 minutes each time; place the substrate in an ultra-clean bench to air dry naturally, and pre-bake on a 95°C hot plate for 10 minutes before use.

[0025] (2) Carry out the gluing of the substrate by the spin coating method: place the SU-8 photoresist in a 55°C water bath and ultrasonically clean it three times for 25 minutes each time before uniform gluing, the purpose is to remove the air bubbles in the photoresist; set The gluing process of the homogenizer is to rotate at a speed of 500 rm / min for 30 sec, and then at a speed of 1000 rm / min for 30 sec to obtain a photoresist layer with a height ranging from 100 to 150 μm.

[0026] (3) The glue-coated substrate is p...

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Abstract

The invention belongs to the fields of the biochemistry, the analysis chemistry, machining and drug screening, and relates to a micro-fluidic chip for screening hepatoenteral circulation drugs. The micro-fluidic chip is formed by four basic units, each of the basic units comprises a fluid inlet, a fluid outlet and a cell culture pool; the cell culturing pool of each of the basic unit I, the basic unit II and the basic unit III is a circle with the diameter of 5mm, and the cell culture pool of the basic unit IV is provided with cylindrical fences; and the micro-fluidic chip concretely comprises, from top to bottom, the basic unit I, a colon cancer cell film, a blood vessel endothelial cell film, the basic unit II, the basic unit III, a blood vessel endothelial cell film, the basic unit IV, a hepatocyte film and a chip. The micro-fluidic hepatoenteral circulation chip adopts multi-layer basic unit integration, complex fluid control and multiple microstructure design to realize the micromation, the integration and the rapid high flux of the screening of the hepatoenteral circulation drugs.

Description

technical field [0001] The invention belongs to the fields of biochemistry, analytical chemistry, mechanical processing and drug screening, and relates to a microfluidic chip for realizing drug screening of enterohepatic circulation. Background technique [0002] Microfluidic chip is a science and technology characterized by the manipulation of fluids in the micron-scale space. It has the ability to shrink the basic functions of biological and chemical laboratories onto a chip of a few square centimeters. Therefore, in theory, microfluidic chips can be applied to any discipline involving fluids, the most immediate of which should be chemistry, biology, and medicine. At the same time, its second-wave influence has penetrated some traditional concepts Disciplines that do not involve fluids, such as optics and informatics. All these disciplines are directly facing the actual needs of various industries in society, and the fields involved include disease diagnosis, drug screeni...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M3/00
CPCB01L3/502707B01L3/502761B01L2200/0631B01L2200/0636B01L2200/0647B01L2200/12B01L2300/0887
Inventor 罗勇孙丽君赵伟杰林炳承田中群
Owner DALIAN UNIV OF TECH
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