A method for introducing exogenous genes into closed-growth pollinated indica rice using pmi screening markers

A technology of exogenous gene and closed-growth pollination, applied in the field of genetic transformation, can solve the problem of screening marker genes without the genetic transformation method of closed-growth pollinated indica rice

Inactive Publication Date: 2017-01-18
RICE RES ISTITUTE ANHUI ACAD OF AGRI SCI
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Problems solved by technology

[0005] Therefore, there is currently no screening marker gene and corresponding transformation method suitable for the genetic transformation method of closed-growth pollinated indica rice varieties.

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  • A method for introducing exogenous genes into closed-growth pollinated indica rice using pmi screening markers
  • A method for introducing exogenous genes into closed-growth pollinated indica rice using pmi screening markers
  • A method for introducing exogenous genes into closed-growth pollinated indica rice using pmi screening markers

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Embodiment Construction

[0054] Unless otherwise specified, the operations in the following specific embodiments are performed by common conventional operations in the art. Those skilled in the art can easily obtain teachings about such routine operations from the prior art, for example, with reference to the textbooks Sambrook and David Russell, Molecular Cloning: A Laboratory Manual, 3rd ed., Vols 1, 2; Charles Neal Stewart, Alisher Touraev, Vitaly Citovsky and Tzvi Tzfira, Plant Transformation Technologies, etc. The medicinal raw materials, reagent materials, etc. used in the following examples are all commercially available products unless otherwise specified.

[0055] Example——Genetic Transformation Method of Clover-pollinated Indica Rice 9m90 Using Mannose as Screening Agent

[0056] 1. Induction and pre-culture of mature embryo callus

[0057] The mature seeds of the closed glume pollinated indica rice 9m90 (preserved by the Rice Research Institute of the Anhui Academy of Agricultural Science...

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Abstract

The invention provides a method for introducing an exogenous gene into a closed glume pollinated indica rice by using a PMI (phosphomannose isomerase, phosphomannose isomerase) screening marker. Specifically, the present invention is mediated by the Agrobacterium strain EHA105 containing the pCAMBIA1381-PMI vector, and using mannose as a screening agent, the exogenous gene is introduced into the indica rice variety 9m90 pollinated by closed glumes. After the introduction, it was confirmed by PCR detection and identification that the exogenous gene had been introduced into 9m90. This method successfully realizes the genetic transformation of indica rice varieties pollinated by closed glumes, and does not require the use of antibiotics or herbicides for screening. While speeding up the process of trait improvement, it avoids gene drift caused by pollen spreading, and improves the degree of environmental friendliness.

Description

technical field [0001] The invention relates to the technical fields of biotechnology and plant genetic engineering. Specifically, the present invention relates to a genetic transformation method for introducing exogenous genes into the clover-pollinated indica rice variety 9m90 by using PMI screening markers. Background technique [0002] Rice (Oryza sativa L.) is the most important food crop in my country and even in the world, and more than 60% of the world's population uses rice as a staple food. In recent years, in the face of increasingly frequent drought, extreme temperature and other abiotic stresses, population growth and ecological environment pressure, the importance of modern biotechnology methods, especially transgenic technology, in crop genetic improvement has become increasingly prominent. Transgenic technology has provided a new path and opened up new space for crop yield improvement, quality improvement and resistance enhancement. [0003] With the rapid ...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/84A01H4/00A01H5/00
Inventor 李浩杨剑波魏鹏程李莉杨亚春倪大虎宋丰顺倪金龙秦瑞英陆徐忠马卉
Owner RICE RES ISTITUTE ANHUI ACAD OF AGRI SCI
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