Method for regenerating plant from camellia callus

Abstract

The invention mainly relates to a method for regenerating a plant from camellia callus. The method has the following steps of stripping off the inner and outer seed coats of a camellia fruit seed, and inoculating the seed to a 1 / 2MS culture medium; when a sterile seedling grows above 3 cm, inoculating the soft leaf of the young plant to a callus induction culture medium which is MS + 0.5mg.L <-1>6-BA+1.0mg.L <-1>2,4-D; when the callus grows to get a diameter about 1 cm, shifting the callus to a callus differentiation culture medium which is MS+mg.L <-1>6-BA20+0.1mg.L <-1>I BA+ mg.L <-1> KT0.1; when an indefinite bud grows to 0.5 cm, carrying out the separation and inoculating to a strong bud culture medium which is MS + 0.2mg.L <-1>6-BA+0.05mg.L <-1>NAA; and when a bud stick grows to 4 to 5cm, cutting off the basal of the bud stick, immersing the basal of the bud stick in 1,000 g.L <-1> I BA, and then inoculating to a MW + 0.2mg.L <-1>I BA+0.2mg.L <-1>NAA culture medium. The method has the advantages that: the method has a simple culture medium recipe, a simple and convenient operating process, short culture time, high regeneration frequency and a high propagation expansion coefficient, and facilitates the large-scale production of rare camellia plants and the realization of the genetic transformation of exogenous genes.

Description

technical field [0001] The invention relates to a camellia tissue culture method, belongs to the field of biotechnology, and is a method for regenerating plants from camellia callus. Background technique [0002] Camellia is a characteristic precious flower in my country and a world-renowned ornamental woody plant. Camellia is an important material for landscaping. It has beautiful flower colors, long flowering period, bright green leaves, colorful tree crowns, and the habit of growing well under tall tree crowns. It is widely used in park green spaces, natural scenic spots and scenic spots. Camellia is also a good potted plant. Certain dwarf shrub varieties are often used as potted plants. In addition to ornamental value, camellia also has high economic value. Its flowers and roots can be used as medicine, and its fruit can be used to extract oil. The yellow extract can be used as food coloring. [0003] In order to maintain the excellent traits of the parents of the cam...

AI Technical Summary

Problems solved by technology

Long Zhenxiong (1981) used the pollen of Camellia oleifera as explants, and obtained a higher callus induction frequency on a suitable medium, and obtained green shoots and roots, but failed to obtain complete regeneration due to pollution plant

[0005] At present, there is no report of obtaining adventitious buds from the camellia callus, and no patent application