Method for production of lactic acid bacteria by utilizing ethanol industrial yellow water

A technology of yellow water and lactic acid bacteria in the alcohol industry, which is applied in the field of microbial cultivation using yellow water in the alcohol industry, can solve the problems of high production cost, increased cost, and impact on the application of lactic acid bacteria, so as to increase production, effectively reduce content, and improve comprehensive Use the effect of value

Inactive Publication Date: 2014-08-27
HENAN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current production methods of lactic acid bacteria mostly use artificially adding a large amount of high-quality nutrients as raw materials for production, which has high production costs, which in turn affects the application of lactic acid bacteria and increases the cost of related products that use lactic acid bacteria as raw materials

Method used

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  • Method for production of lactic acid bacteria by utilizing ethanol industrial yellow water
  • Method for production of lactic acid bacteria by utilizing ethanol industrial yellow water
  • Method for production of lactic acid bacteria by utilizing ethanol industrial yellow water

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Embodiment 1: a kind of method utilizing alcohol industrial yellow water to produce lactic acid bacteria, what this method adopted is that corn is the yellow water of raw material production alcohol, and concrete steps are as follows:

[0030] (1) Filter the collected alcohol industrial yellow water with general medicinal gauze (double layers), and collect the filtrate as a culture substrate for lactic acid bacteria for later use;

[0031] (2) Determination of the nutritional components of the filtrate obtained in step (1), the determination results are shown in the attached figure 1 , and carry out nutritional supplementation according to the measurement results and the nutritional needs for the growth and reproduction of lactic acid bacteria. The types and quantities of specific nutritional supplements are as follows: add 2.5g of urea, 10g of glucose, and 0.5mL of Tween 80 per liter of the above-mentioned alcohol industrial yellow water;

[0032] (3) Sterilize the nut...

Embodiment 2

[0038] Embodiment 2: a kind of method utilizing the yellow water of alcohol industry to produce lactic acid bacteria, what this method adopted is that corn is the yellow water of raw material production alcohol, and concrete steps are as follows:

[0039] (1) Filter the collected alcohol industrial yellow water with general medicinal gauze (double layers), and collect the filtrate as a culture substrate for lactic acid bacteria for later use;

[0040] (2) Determination of the nutritional components of the filtrate obtained in step (1), the determination results are shown in the attached figure 1 , and carry out nutritional supplementation according to the measurement results and the nutritional needs for the growth and reproduction of lactic acid bacteria. The types and quantities of specific nutritional supplements are as follows: 2.5g of urea, 10g of glucose, and 0.5mL of Tween 80 are added to each liter of the above-mentioned alcohol industrial yellow water filtrate;

[004...

Embodiment 3

[0047] Embodiment 3: a kind of method utilizing the yellow water of alcohol industry to produce lactic acid bacteria, what this method adopted is that corn is the yellow water of raw material production alcohol, and concrete steps are as follows:

[0048] (1) Filter the collected alcohol industrial yellow water with general medicinal gauze (double layers), and collect the filtrate as a culture substrate for lactic acid bacteria for later use;

[0049] (2) Determination of the nutritional components of the filtrate obtained in step (1), the determination results are shown in the attached figure 1 , and carry out nutritional supplementation according to the measurement results and the nutritional needs for the growth and reproduction of lactic acid bacteria. The types and quantities of specific nutritional supplements are as follows: 2.5g of urea, 10g of glucose, and 0.5mL of Tween 80 are added to each liter of the above-mentioned alcohol industrial yellow water filtrate;

[005...

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Abstract

A method for production of lactic acid bacteria by utilizing ethanol industrial yellow water includes collection and treatment of the ethanol industrial yellow water, cultivation of the lactic acid bacteria in the ethanol industrial yellow water, and receiving and preparation of the lactic acid bacteria. The method for production of the lactic acid bacteria by utilizing the ethanol industrial yellow water not only can make full use of the ethanol industrial yellow water, reduce the treatment cost of the yellow water, optimize comprehensive utilization of the yellow water and improve the ecological environment, but also can expand sources of a nutrition substrate in a conventional lactic acid bacteria production, lower the production costs and expand the application scope of lactic acid bacteria.

Description

technical field [0001] The invention relates to the field of comprehensive utilization of yellow water in the alcohol industry and the field of microorganism cultivation, in particular to the use of yellow water in the alcohol industry for microorganism cultivation. Background technique [0002] Alcohol industry is an important energy industry in my country, but huge by-products will be produced in the process of alcohol production, among which solid components such as distiller's grains and part of yellow water can continue to produce feed materials such as DDGS and be reused, and most of them are The yellow water has not been well utilized. Yellow water is a liquid substance rich in various nutrients discharged during the production of alcohol. Yellow water is rich in organic acids (especially carboxylic acids such as lactic acid, butyric acid, caproic acid, and acetic acid), alcohols, aldehydes, At the same time, it also contains amino acids, sugars, alcohol, humus, yeast ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12R1/01
Inventor 何万领张广勤李旺丁轲李元晓李晓丽
Owner HENAN UNIV OF SCI & TECH
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