Preparation method of Fe3O4@Au-BSA magnetic nano-compound and chiral separation use thereof

A fe3o4au-, fe3o4au-bsa technology, applied in other chemical processes, laboratory containers, laboratory utensils, etc., can solve the problems of limited chiral separation applications, expensive chromatographic columns, short service life, etc., and achieves the method Simple and fast, good magnetic response performance, simple and controllable fixation effect

Inactive Publication Date: 2014-09-17
NANCHANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Chromatography is a common tool for chiral separation, but its application in chiral separation is limited due to the high price of chromatographic columns, short service life, large sample volume and even racemization.

Method used

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  • Preparation method of Fe3O4@Au-BSA magnetic nano-compound and chiral separation use thereof
  • Preparation method of Fe3O4@Au-BSA magnetic nano-compound and chiral separation use thereof
  • Preparation method of Fe3O4@Au-BSA magnetic nano-compound and chiral separation use thereof

Examples

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Embodiment 1

[0023] Fabrication of PDMS chip: Using the GaAs positive mold (produced by the 55th Institute of Electronics in Nanjing) as a template, fabricate a typical cross-shaped PDMS microfluidic chip channel, such as figure 1 shown. The specific production process is as follows: Take a certain amount of PDMS monomer and curing agent according to 10:1 (mass ratio), mix evenly, degas, pour on the GaAs male mold, and cure at 70 oC for 2 hours. After cooling, peel off the PDMS chip containing the cross-shaped channel from the template, cut it into the desired shape with a blade, and punch holes in the buffer pool, sample pool and sample waste pool with a puncher to form a diameter of 3 mm holes. At the same time, using flat glass as a template, the same procedure was followed to prepare a PDMS chip without microchannels as a cover slip. The PDMS chip containing the cross channel and the PDMS cover sheet without the channel were ultrasonically cleaned with secondary water, methanol, and ...

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Abstract

The invention discloses a synthesis method of Fe3O4 magnetic nanoparticles coated with gold and its use in chiral selector assembling, stationary phase preparation and chiral amino acid separation analysis. Through an ultrasonic chemical method, gold nanoparticles coat the surfaces of the Fe3O4 magnetic nanoparticles so that Fe3O4@Au core-shell nanoparticles having good magnetism and good biocompatibility are obtained, through gold-ammonia bond action, bovine serum albumin is fixed to the surfaces of the Fe3O4@Au nanoparticles, and under the action of an external magnetic field, Fe3O4@Au-bovine serum albumin can be controllably fixed in a separation channel of a micro-fluidic chip so that a micro-fluidic chip with Fe3O4@Au-bovine serum albumin as a chiral stationary phase is obtained.

Description

technical field [0001] The present invention relates to a kind of Fe 3 o 4 The preparation method of Au-BSA magnetic nanocomposite and its application in chiral amino acid separation belong to the field of microfluidic chip technology. Background technique [0002] Chirality is one of the most important properties in nature and plays an extremely important role in life activities. Because of their important physiological functions and special physicochemical and physiological properties, amino acids have become a research hotspot in enantiomer resolution. Chromatography is a common tool for chiral separation, but its application in chiral separation is limited due to the disadvantages of high price, short service life, large sample volume and even racemization of chromatographic columns. Capillary electrophoresis has the advantages of rapidity and low consumption, and has become an active field of chiral separation research. Microfluidic chip electrophoresis technology i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01J20/29B01J20/30B01L3/00C07D209/20
Inventor 邱建丁王莉王晓妮
Owner NANCHANG UNIV
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