Preparation method of traditional Chinese medicine micro-ecological additive for prevention and treatment of subclinical mastitis of cows

Abstract

Disclosed is a preparation method of a traditional Chinese medicine micro-ecological additive for prevention and treatment of subclinical mastitis of cows. The preparation method comprises performing superfine grinding on eucommia ulmoides leaves, ginkgo leaves, purple perilla, Chinese pulsatilla roots, coneflowers, humulus scandens, shrimp shells, milkvetch roots and licorice roots; adding water and boiling through the high heat; performing soft file insulation; performing cooling and filtration; collecting filtrate; adding white sugar and boiling; cooling down to the room temperature and performing lactobacillus, tea fungi and yeast inoculation to obtain the micro-ecological additive through fermentation; enabling the cows to drink the obtained micro-ecological additive in proportion. According to the traditional Chinese medicine micro-ecological additive, the functions of heat clearing and detoxification, sterilization and anti-inflammation, lactation promotion and the like are achieved, Chinese herbal medicine active materials, active small peptides, lactic acid and probiotics are rich, the amino acid is rich and balanced, the traditional antibiotic treatment method is replaced, and the harm of antibiotics to the health of the cows and antibiotic residues in the milk are reduced. According to the preparation method of the traditional Chinese medicine micro-ecological additive for the prevention and treatment of the subclinical mastitis of the cows, the use is simple and convenient, drug residues are not produced, any toxic and side effect is not produced, the viable bacteria content is high, the preparation method is suitable for industrialized production, and the market prospects are good.

Description

technical field

[0001] The invention belongs to the technical field of feed microbial fermentation, and in particular relates to a preparation method of a traditional Chinese medicine microecological additive for preventing and treating cow recessive mastitis. Background technique

[0002] Cow mastitis (Mastitis) is an inflammatory pathological change of the mammary gland due to physical, chemical, and microbial stimulation. Its main feature is the physical and chemical properties and bacteriological changes of the milk. One of the most serious diseases. According to the presence or absence of clinical symptoms, dairy cow mastitis is divided into two types: clinical mastitis and subclinical mastitis. Clinically, pathogenic microorganism infection is the main pathogenic factor, and pathogenic microorganisms mainly include Staphylococcus aureus, Streptococcus agalactiae, Streptococcus dysgalactiae, Escherichia coli, Streptococcus uberis, Klebsiella pneumoniae and so on. Infe...

Examples

Embodiment 1

[0022] A method for preparing a traditional Chinese medicine microecological additive for preventing and treating cow recessive mastitis, comprising the following steps:

[0023] Weigh the following Chinese herbal medicines in parts by weight (Kg): 120 Eucommia leaves, 120 Ginkgo leaves, 100 perilla, 100 Pulsatilla chinensis, 100 Echinacea purpurea, 100 Humulus chinensis, 80 shrimp shells, 60 astragalus, 40 licorice, and ultrafinely pulverize.

[0024] Preparation of fermented liquid: add 6 times the amount of water of the total weight of Chinese herbal medicine, soak for 30 minutes, boil with strong fire, keep warm for 30 minutes with slow fire, cool and filter with 12 layers of gauze; Filter through two layers of gauze, and combine the two filtrates. Add 15Kg of white sugar to 100 Kg of filtrate as a unit, boil for 5 minutes, cool to room temperature, inoculate 15Kg of Lactobacillus, Kombucha and yeast preparations, and ferment in a sterile fermentation room at 35°C for 7 da...

Embodiment 2

[0027] A method for preparing a traditional Chinese medicine microecological additive for preventing and treating cow recessive mastitis, comprising the following steps:

[0028] Weigh the following Chinese herbal medicines by weight (Kg): Eucommia leaf 150, Ginkgo biloba 150, Perilla 130, Pulsatilla 130, Echinacea 120, Humulus 120, Shrimp shell 100, Astragalus 80, Licorice 60, ultrafinely pulverized.

[0029] Preparation of fermentation broth: add 8 times the amount of water of the total weight of Chinese herbal medicine, soak for 60 minutes, boil with strong fire, keep warm for 60 minutes with slow fire, cool and filter with twelve layers of gauze; Filter through two layers of gauze, and combine the two filtrates. Add 20Kg of white sugar to 100 Kg of filtrate as a unit, boil for 10 minutes, cool to room temperature, inoculate 30Kg of Lactobacillus, Kombucha and yeast preparations, and ferment in a sterile fermentation room with a fermentation temperature of 37°C, a fermentat...

technical field

[0001] The invention belongs to the technical field of feed microbial fermentation, and in particular relates to a preparation method of a traditional Chinese medicine microecological additive for preventing and treating cow recessive mastitis. Background technique

[0002] Cow mastitis (Mastitis) is an inflammatory pathological change of the mammary gland due to physical, chemical, and microbial stimulation. Its main feature is the physical and chemical properties and bacteriological changes of the milk. One of the most serious diseases. According to the presence or absence of clinical symptoms, dairy cow mastitis is divided into two types: clinical mastitis and subclinical mastitis. Clinically, pathogenic microorganism infection is the main pathogenic factor, and pathogenic microorganisms mainly include Staphylococcus aureus, Streptococcus agalactiae, Streptococcus dysgalactiae, Escherichia coli, Streptococcus uberis, Klebsiella pneumoniae and so on. Infe...

Examples

Embodiment 1

[0022] A method for preparing a traditional Chinese medicine microecological additive for preventing and treating cow recessive mastitis, comprising the following steps:

[0023] Weigh the following Chinese herbal medicines in parts by weight (Kg): 120 Eucommia leaves, 120 Ginkgo leaves, 100 perilla, 100 Pulsatilla chinensis, 100 Echinacea purpurea, 100 Humulus chinensis, 80 shrimp shells, 60 astragalus, 40 licorice, and ultrafinely pulverize.

[0024] Preparation of fermented liquid: add 6 times the amount of water of the total weight of Chinese herbal medicine, soak for 30 minutes, boil with strong fire, keep warm for 30 minutes with slow fire, cool and filter with 12 layers of gauze; Filter through two layers of gauze, and combine the two filtrates. Add 15Kg of white sugar to 100 Kg of filtrate as a unit, boil for 5 minutes, cool to room temperature, inoculate 15Kg of Lactobacillus, Kombucha and yeast preparations, and ferment in a sterile fermentation room at 35°C for 7 da...

Embodiment 2

[0027] A method for preparing a traditional Chinese medicine microecological additive for preventing and treating cow recessive mastitis, comprising the following steps:

[0028] Weigh the following Chinese herbal medicines by weight (Kg): Eucommia leaf 150, Ginkgo biloba 150, Perilla 130, Pulsatilla 130, Echinacea 120, Humulus 120, Shrimp shell 100, Astragalus 80, Licorice 60, ultrafinely pulverized.

[0029] Preparation of fermentation broth: add 8 times the amount of water of the total weight of Chinese herbal medicine, soak for 60 minutes, boil with strong fire, keep warm for 60 minutes with slow fire, cool and filter with twelve layers of gauze; Filter through two layers of gauze, and combine the two filtrates. Add 20Kg of white sugar to 100 Kg of filtrate as a unit, boil for 10 minutes, cool to room temperature, inoculate 30Kg of Lactobacillus, Kombucha and yeast preparations, and ferment in a sterile fermentation room with a fermentation temperature of 37°C, a fermentat...

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