Process method for extracting chlorogenic acid from blumea riparia(BL.)DC

A process method, the technology of chlorogenic acid, is applied in chemical instruments and methods, the separation/purification of carboxylate, and the preparation of carboxylate, which can solve the problems of low preparation purity, complicated preparation process, and difficult industrial production. Achieve the effects of simple process steps, simple operation and constant yield

Active Publication Date: 2014-11-05
广西万寿堂药业有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] In order to solve the above technical problems, the present invention provides a process for extracting chlorogenic acid from Diangui Ainaxiang, which solves the problems of low preparation purity, small preparation amount, complicated preparation process and difficulty in realizing industrialized production. The combination of porous resin column enrichment and mixed solvent phase separation method realizes the distribution gradient purification of chlorogenic acid, the extraction purity is high, and the process steps are simple, suitable for industrial production

Method used

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  • Process method for extracting chlorogenic acid from blumea riparia(BL.)DC
  • Process method for extracting chlorogenic acid from blumea riparia(BL.)DC
  • Process method for extracting chlorogenic acid from blumea riparia(BL.)DC

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Embodiment 1

[0034] The process of extracting chlorogenic acid from Ainaxiang osmanthus Dianensis:

[0035]Put the impurity-removed medicinal material of Yunnan Gui Ainaxiang into an extraction tank, add water to decoct twice, add 10 times the amount of water for decoction for 1.5 hours for the first time, and decoct for 1 hour with 10 times the amount of water for the second time, and control the temperature at 65°C, filter with a 200-mesh filter cloth, combine the filtrate; concentrate the filtrate to a concentrated solution with a relative density of 1.08 at 60°C, move it to a sedimentation tank, let it stand for 8 hours, and process it by sedimentation and centrifugation, take the clarified filtrate and concentrate it to a relative density of 60°C An extract with a density of 1.36; the extract is dissolved in 5 times the amount of water and heated to 65°C, filtered to obtain an aqueous solution of the extract, adjusted to pH 3, added to a polar macroporous resin column for saturated ads...

Embodiment 2

[0037] The process of extracting chlorogenic acid from Ainaxiang osmanthus Dianensis:

[0038] Put the impurity-removed medicinal material of Yunnan Gui Ainaxiang into an extraction tank, add water to decoct twice, add 15 times the amount of water for decoction for 2 hours for the first time, and decoct for 1.5 hours with 10 times the amount of water for the second time, and control the temperature at 80°C, filter with a 230-mesh filter cloth, and combine the filtrates; concentrate the filtrate to a concentrated solution with a relative density of 1.08 at 65°C, move it to a sedimentation tank, and let it stand for 8 hours. An extract with a density of 1.38; the extract is dissolved in 8 times the amount of water and heated at 80°C, filtered to obtain an aqueous solution of the extract, adjusted to pH 5, added to a polar macroporous resin column for saturated adsorption, and the adsorption flow rate is controlled 10ml / min, the total amount of the extract is controlled as 1 time...

Embodiment 3

[0040] The process of extracting chlorogenic acid from Ainaxiang osmanthus Dianensis:

[0041] Put the impurity-removed medicinal material of Yunnan Gui Ainaxiang into an extraction tank, add water to decoct twice, add 12 times the amount of water to decoct for 1.5 hours for the first time, add 8 times the amount of water to decoct for 1 hour for the second time, and control the temperature as follows: 60°C, filter with a 180-mesh filter cloth, and combine the filtrates; concentrate the filtrate to a concentrated solution with a relative density of 1.08 at 58°C, move it to a sedimentation tank, and let it stand for 8 hours. An extract with a density of 1.36; the extract is dissolved in 6 times the amount of water and heated at 70°C, filtered to obtain an aqueous solution of the extract, adjusted to pH 4, added to a polar macroporous resin column for saturated adsorption, and the adsorption flow rate is controlled It is 6ml / min, and the total amount of extract is controlled as ...

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Abstract

The invention discloses a process method for extracting chlorogenic acid from blumea riparia(BL.)DC, and belongs to the technology field of traditional Chinese medicine extraction process. According to the method, a chlorogenic acid monomer is prepared from the following process: water extraction, concentration, macroporous resin adsorption and separation, elution, purification, and further purification, wherein a compound C is analyzed and determined to be chlorogenic acid with the content of not less than 98.6% by HPLC and is the chlorogenic acid monomer. The process method for extracting the chlorogenic acid from blumea riparia(BL.)DC solves the problems that the preparation purity is not high, the preparation amount is small, the preparation course is complicated and industrialized production is difficult to realize; with combination of the polar macroporous resin column enrichment and a mixed solvent split-phase method, the chlorogenic acid distribution gradient purification is realized, the extraction purity is high, the process steps are simple, and the process method is suitable for industrialized production.

Description

technical field [0001] The invention relates to the technical field of extracting traditional Chinese medicines, in particular to a process for extracting chlorogenic acid from yunnangui ainaxiang. Background technique [0002] Diangui Ainaxiang Blumea riparia (BL.) DC, also recorded in "Flora of China", is called False Dongfengcao [1], and the whole herb is commonly used as medicine by folks. It is recorded in "Chinese Medicine Sea" that the nature and flavor of Diangui Ainaxiang belong to the meridians: light, sweet and flat, enter the three meridians of liver, kidney and bladder; efficacy and indications to promote blood circulation and stop bleeding - this product is sweet and light into the liver, nourishes the liver and activates blood circulation, astringent and hemostasis , has the function of promoting blood circulation without hurting blood, stopping bleeding without leaving silt, and can be used for various hemorrhages; diuresis and swelling—this product is sweet ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C69/732C07C67/48C07C67/56
CPCC07C67/48C07C67/56C07C2601/14C07C69/732
Inventor 毛金玲雷飞凤农常东罗盛东
Owner 广西万寿堂药业有限公司
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