Pharmaceutical composition comprising nelumbo nucifera seed extract as active ingredient for protecting brain nerve cells

A technology of brain nerve cells and production methods, which is applied in the field of pharmaceutical compositions for brain nerve cell protection, can solve problems such as the protective activity of lotus seed heart extracts that have not been mentioned, and achieve excellent brain nerve cell protection effects and excellent brain nerve cell protection. The effect of nerve cell protective activity

Inactive Publication Date: 2014-11-12
NAT INST OF BIOLOG RESOURCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, none of the above patents mentioned that lotus seed extract has brain nerve cell protective activity.

Method used

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  • Pharmaceutical composition comprising nelumbo nucifera seed extract as active ingredient for protecting brain nerve cells
  • Pharmaceutical composition comprising nelumbo nucifera seed extract as active ingredient for protecting brain nerve cells
  • Pharmaceutical composition comprising nelumbo nucifera seed extract as active ingredient for protecting brain nerve cells

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Embodiment 1: the extraction method of lotus divided layer

[0051] Ultrasonic extraction is performed on lotus seeds, seed coats, lotus seed hearts and cotyledons in various parts of the lotus flower. The extraction solvent is 80% methanol, and the amount of solvent is 1L of solvent per 100g of sample. The extraction time is 90 minutes per sample, and a total of 3 extractions, and the ultrasonic frequency is 42kHz. The extraction yields of each part were 4.15% for lotus seed, 9.25% for seed coat, 44.06% for lotus seed heart and 19.76% for cotyledon.

[0052] Lianzixin utilizes hexane, CHCl in order of solvent polarity from low to high 3 , EtOAc, n-BuOH solvent partitioning. The amount divided into each layer is 0.76g of hexane layer, CHCl 3 Layer 1.00 g, EtOAc layer 0.41 g, n-BuOH layer 2.65 g.

Embodiment 2

[0053] Embodiment 2: the brain nerve cell protection activity of each position of lotus flower

[0054] The protective activity of brain nerve cells was determined using a mouse hippocampus cell line, namely HT22 cells. The viability of HT22 cells was determined by MTT assay. To prepare for MTT assay, HT22 cells were plated 3×10 on a 48well plate 4 After seeding the cells / wells, they were cultured at 37° C. for 24 hours. After culturing, the samples were added at different concentrations before being treated with glutamate, and after culturing for 1 hour, they were treated with glutamate. Then, after culturing at 37°C for 24 hours, MTT solution was added, and after 3 hours, it was dissolved with DMSO, and then the absorbance at 570 nm was measured using an enzyme-linked immunosorbent assay (ELISA) reader. , the results showed that by measuring the protective activity of brain nerve cells in various parts of the lotus flower, the lotus seed heart part showed a 90.84% ​​cytop...

Embodiment 3

[0055] Embodiment 3: the brain nerve cell protection activity of each division layer of lotus seed heart

[0056] In order to measure the protective activity of brain nerve cells in various parts of the lotus flower, the most active lotus seed heart was divided first, and then the cytoprotective activity of each divided layer was measured. The measurement results show that the cell protection activity of the lotus seed heart ethyl acetate (EtoAc) layer is the strongest under the condition of 100ug / ml, reaching 131.82% ( figure 2 , image 3 ).

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Abstract

The present invention relates to a pharmaceutical composition and food composition comprising Nelumbo nucifera seed extract as an active ingredient for the protection of brain nerve cells. Particularly, the composition is characterized by having an activity of removing reactive oxygen species (ROS) or an antioxidant activity. More particularly, the antioxidant activity is characterized by the activity of removing 2,2-diphenyl-1-picrylhydrazyl (DPPH) radicals or the activity of removing hydrogen peroxide (H2O2). Also, the present invention is extracted from a natural substance and is thus safe for the human body and provides fundamental data to the food and pharmaceutical fields, which use products derived from natural substances.

Description

technical field [0001] The invention relates to a pharmaceutical composition for protecting brain nerve cells containing lotus seed heart extract as an active ingredient. Background technique [0002] All parts of the lotus flower of the lotus family have very prominent functions and curative effects in traditional Chinese medicine formulas. Roemerine and nuciferine have good analgesic and sedative effects. Folks not only use it to treat pneumonia, asthma, gonorrhea, physical weakness, indigestion and other diseases, but also use it to treat snake and poisonous insect bites. Studies have shown that it helps to strengthen the body, eliminate fatigue, calm the mind, and long-term consumption can improve health. Lotus seed meat refers to the medicinal material that peels off the seed coat of the lotus seed and dries it. In Japan, this plant is called lotus meat. In China, the seed of this plant is called lotus seed. The dry young leaves and young roots in the mature seeds It ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/62A61P25/28A61P25/00
CPCA61K36/62A23L33/105A61P25/00A61P25/28E04G11/36E04G25/04E04G2001/242A23V2002/00A61K36/48
Inventor 金银实马忠制吴庚姬
Owner NAT INST OF BIOLOG RESOURCES
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