SSR molecular markers cosegregated from cucumber powdery mildew-resistant major QTL

A technology of cucumber powdery mildew and molecular markers, which is applied in the field of genetic engineering, can solve the problems of difficulty in QTL positioning and achieve the effect of accelerating the process and promoting cloning

Inactive Publication Date: 2014-11-19
SHANGHAI JIAO TONG UNIV
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  • Abstract
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Problems solved by technology

[0007] The purpose of the present invention is to overcome the difficulty of QTL positioning and provide three co-do

Method used

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  • SSR molecular markers cosegregated from cucumber powdery mildew-resistant major QTL
  • SSR molecular markers cosegregated from cucumber powdery mildew-resistant major QTL
  • SSR molecular markers cosegregated from cucumber powdery mildew-resistant major QTL

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Experimental program
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Example Embodiment

[0021] 1. Identification of major QTL / genes for cucumber powdery mildew resistance

[0022] 1. Group construction and resistance identification

[0023] The initial resistance QTL mapping used F 2 In the population, the resistant parent is S1003 and the susceptible parent is S1001. They are all cucumber inbred lines of North China. F produced by cross between two parents 1 Generation F 2 Generation groups. The bacterial strain used for the identification of powdery mildew resistance was isolated from cucumber plants with disease in the greenhouse of Shanghai Jiao Tong University. Randomly select 148 F 2 Individuals of the next generation are tested for resistance at the seedling stage. Collect purified powdery mildew bacteria from infected seedlings, prepare a spore suspension with sterile water, and adjust the concentration to 1×10 5 A·mL -1 . When the third true leaf of cucumber is just unfolded, spray the prepared spore suspension evenly on the true leaf, whichever is not wat...

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Abstract

The invention discloses three SSR molecular markers cosegregated from a cucumber powdery mildew-resistant major QTL. The three SSR molecular markers are respectively named as SSR-N1, SSR-N2 and SSR-N3, wherein the nucleotide sequence of the SSR-N1 is shown in the SEQ ID No.1; the nucleotide sequence of the SSR-N2 is shown in the SEQ ID No.2; the nucleotide sequence of the SSR-N3 is shown in the SEQ ID No.3. The three SSR molecular markers disclosed by the invention all have high stability, can be simply and rapidly applied to auxiliary screening of powdery mildew-resistant individual plants at the cucumis sativus seedling stage, and lay a foundation for assistant breeding of powdery mildew-resistant molecular markers, so that the process of breeding cucumber powdery mildew-resistant molecules is accelerated greatly. Moreover, the cosegregated molecular markers lay a foundation for cloning of the cucumber powdery mildew-resistant major QTL.

Description

Technical field [0001] The invention relates to genetic engineering technology, in particular to an SSR molecular marker co-segregated with a major QTL for cucumber powdery mildew resistance. Background technique [0002] Cucumber (Cucumis sativus L.) is an annual herbaceous creeping climbing plant in the Cucurbitaceae melon family. As one of the top ten important vegetable crops in the world, cucumber is also one of the main vegetable crops in my country, accounting for about 10% of the country's vegetable area. Cucumber is not only an important vegetable crop that has always been valued by breeders, but the number of chromosomes of cucumber is less 2n=2x=14, and the genome is small. Especially in 2009, the successful sequencing of cucumber genome was carried out as a model plant of Cucurbitaceae. Molecular biology research provides great convenience. [0003] In cucumber production, it faces many diseases, among which powdery mildew is one of the most serious diseases. Cucumbe...

Claims

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Application Information

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IPC IPC(8): C12N15/11C12Q1/68
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 蔡润聂京涛潘俊松何欢乐杨俊俊彭佳林
Owner SHANGHAI JIAO TONG UNIV
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