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Method for detecting ultralow content of aflatoxin

A technology for aflatoxin and content, which is applied in measuring devices, instruments, material analysis by electromagnetic means, etc., can solve the problems of narrow detection range and low sensitivity, and achieve the effects of simple equipment, simple operation and good development space.

Inactive Publication Date: 2014-12-03
CHONGQING MEDICAL UNIVERSITY
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Problems solved by technology

[0009] The purpose of the embodiments of the present invention is to provide a method for detecting ultra-low levels of aflatoxin, aiming to solve the problems of low sensitivity and narrow detection range of current methods for detecting aflatoxin

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  • Method for detecting ultralow content of aflatoxin
  • Method for detecting ultralow content of aflatoxin
  • Method for detecting ultralow content of aflatoxin

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Embodiment Construction

[0025] In order to make the object, technical solution and advantages of the present invention more clear, the present invention will be further described in detail below in conjunction with the examples. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.

[0026] figure 1 The flow chart of the method for detecting ultra-low content aflatoxin provided by the present invention is shown. For ease of illustration, only the parts relevant to the present invention are shown.

[0027] Such as figure 1 Shown, the method for the detection ultra-low content aflatoxin that the embodiment of the present invention provides comprises the following steps:

[0028] Prepare a glassy carbon electrode, chemically modify a layer of single-walled carbon nanotubes on the glassy carbon electrode, and use a linker to covalently bond;

[0029] Coupling of bovine serum albumin-aflatoxin to form an...

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Abstract

The invention discloses a method for detecting ultralow content of aflatoxin. The method comprises the following steps: preparing a glassy carbon electrode, chemically modifying the glassy carbon electrode with a layer of single-walled carbon nanotubes, and covalently bonding by using a bridging agent; coupling bovine serum albumin with aflatoxin to form antigen AFB1-BSA; reacting and connecting the antigen AFB1-BSA with the activated carboxyl group on the single-walled carbon nanotubes; competitively adsorbing a primary antibody through using an antigen-antibody reaction and an added aflatoxin monomer by adopting a sandwich technology and a competitive technology to obtain a secondary antibody; labeling the secondary antibody by alkaline phosphatase; determining the electrochemical signal of the alkaline phosphatase labeled secondary antibody, and drafting a standard curve to obtain the concentration of aflatoxin in an actual measurement sample. The method for detecting ultralow content of aflatoxin has the advantages of simple operation, high selectivity, high specificity, simple and rapid detection of the ultralow content of aflatoxin, simple required device, low cost, great actual use values and good development space.

Description

technical field [0001] The invention belongs to the field of aflatoxin detection, in particular to a method for detecting ultra-low content of aflatoxin. Background technique [0002] Aflatoxin (AFT) is mainly a secondary metabolite produced by fungi such as Aspergillus flavus and Aspergillus parasiticus. Aflatoxin is divided into B1, B2, G1, G2 and M1, M2 several subtypes, of which AFB1 is listed as a class I carcinogen by the International Agency for Research on Cancer, the most toxic, 10 times that of potassium cyanide, 68 times that of arsenic Long-term intake of low doses or single exposure of large doses can cause liver cancer or acute poisoning in various animals. Low-dose long-term intake or high-dose exposure can cause liver cancer or acute poisoning in many animals. [0003] The physical and chemical properties of AFB1 are very stable. It decomposes at a high temperature of 268-269 °C, and the toxicity is reduced by half only after 4 hours of high-pressure 0.103 ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/551G01N27/327
CPCG01N33/551G01N27/3278G01N33/54366
Inventor 李朝睿张弦邱景富
Owner CHONGQING MEDICAL UNIVERSITY
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