A kind of vitrification cryopreservation method of flax seeds

A vitrification ultra-low temperature, flax seed technology, applied in the direction of plant preservation, botany equipment and methods, applications, etc., to achieve the effect of simple process, reliable stability, simple and convenient operation
CN104222072BActive Publication Date: 2016-06-29INST OF BAST FIBER CROPS CHINESE ACADEMY OF AGRI SCI
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
INST OF BAST FIBER CROPS CHINESE ACADEMY OF AGRI SCI
Publication Date
2016-06-29

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Abstract

The invention discloses a vitrification ultra-low temperature preservation method of flax seeds. The specific method steps are as follows: take dried fresh flax seeds, place them in a loading solution for 20 minutes at normal temperature, and then transfer them to a frozen container filled with a vitrification protective agent. In the storage tube, the cryopreservation tube was placed in a gradient cooling box, down to -30°C--60°C, and put into liquid nitrogen for ultra-low temperature preservation. Take out the cryopreservation tube from the liquid nitrogen. When the room temperature of flax seeds is greater than or equal to 20°C, thaw naturally. If it is less than 20°C, thaw quickly in a 30°C water bath for 1 minute, wash 2-3 times with water, and then grow directly. The invention has the advantages of simple process, convenient operation, stable genetic properties of the preserved flax seeds, a germination rate of 98.67% in a seed germination test, and long-term preservation of the flax seeds.
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Description

technical field

[0001] The invention belongs to the technical field of flax germplasm resource preservation, and in particular relates to a vitrification ultra-low temperature preservation method of flax seeds. Background technique

[0002] Flax (Linumusitatissimum) is an ancient bast fiber crop and oil crop in the world, which originated in the Middle East and the Mediterranean coast. Flax has a long history of cultivation in China and is a characteristic economic crop. Flax fiber has the best effect among the five hemp spinning industrial raw material crops, and is known as the queen of hemp. Flax germplasm resources are the basic work of flax breeding, scientific research, production and teaching. The primary task of germplasm resources research is to collect germplasm and preserve germplasm safely. At present, the preservation methods of flax germplasm resources are normal temperature and low temperature preservation of flax seeds. Flax seeds stored at room temperature...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
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