A kind of pharmaceutical composition for treating leukemia and preparation method thereof

A composition and leukemia technology, applied in the field of medicine, can solve the problems of many side effects, large doses, and insufficient curative effect, and achieve the effect of prolonging survival time and remarkable effect

Inactive Publication Date: 2016-04-06
淄博市食品药品检验所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Chemotherapy has obvious effects, but its side effects are also more
Chinese patent medicine adjusts the functions of the human body to achieve the balance of yin and yang. Some ingredients in the medicine can enhance immunity, but its curative effect is not fast enough, and there are disadvantages such as large doses.

Method used

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  • A kind of pharmaceutical composition for treating leukemia and preparation method thereof
  • A kind of pharmaceutical composition for treating leukemia and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

experiment example 1

[0021] Experimental example 1, pharmaceutical composition of the present invention is to L 615 Drug Efficacy Test in Leukemia Mice

[0022] 1. Sample preparation

[0023] 1. Extraction preparation

[0024] (1) Preparation of volatile oil and its clathrate: take 150g of succulent grass and 98g of Ganqing Qinglan, add 2500ml of water, use steam distillation to prepare volatile oil (3h), filter, and store the filtrate and filter residue separately;

[0025] (2) Add 5ml of collected volatile oil to 30g of β-cyclodextrin to clathrate the volatile oil to obtain 33g of volatile oil clathrate;

[0026] (3) Take the filtrate obtained in step (1), concentrate under reduced pressure to a clear paste with a relative density of 1.15-1.25 at 50° C., add ethanol to adjust to a volume percentage of 70% alcohol concentration, leave it at 2° C. for 24 hours, and filter. Discard the precipitate to obtain filtrate A;

[0027] (4) Take the herb dregs obtained in step (1), add 300g of cassia yu...

experiment example 2

[0053] Experimental Example 2: For P 388 Pharmacodynamics Test in Leukemia Mice

[0054] 1. Modeling method: ascites type P 388 Leukemic mice were sacrificed by dislocation of the cervical spine, abdomen was disinfected, ascites was sucked, mixed with normal saline, and the cell concentration was adjusted under a microscope to be (1-1.5)*10(^7) cells / ml, 0.2ml per mouse (Kunming species) Inoculation concentration and dose of mice) were injected intraperitoneally.

[0055] 2. To P 388 The effect of natural killer cell (NKC) and interleukin-2 (IL-2) activity after chemotherapy in leukemia mice.

[0056] Materials: 50 first-class Kunming mice (Shandong Experimental Animal Center), 50 males and 50 males, weighing 18-22 g. Samples 1-4 (same as experimental example 1 sample, batch numbers are 20140321, 20140322, 20140323 and 20140324 respectively), cyclophosphamide (Jiangsu Hengrui Medicine Co., Ltd.), standard recombinant human interleukin-2 (Beijing Shuanglu Pharmaceutical Co....

Embodiment 1

[0069] A pharmaceutical composition for treating leukemia, which is composed of the following raw materials: 300g of Plum japonica, 175g of plateau buttercup, 150g of succulent grass, and 98g of Ganqing Qinglan.

[0070] Its preparation method is:

[0071] (1) Take the succulent grass and Ganqing Qinglan, add 10 times the volume of water of the total weight of the medicinal materials, use steam distillation to decoct for 5 hours, collect the volatile oil, filter, and store the filtrate and filter residue for later use;

[0072] (2) 5.5 ml of the collected volatile oil was clathrated with β-cyclodextrin with a volume-to-weight ratio of 6 times the weight to obtain 36 g of volatile oil clathrate;

[0073] (3) Take the filtrate obtained in step (1), concentrate it under reduced pressure to a clear paste with a relative density of 1.20 at 50° C., add ethanol to adjust to a volume percentage of 70% alcohol concentration, leave it at 2-10° C. for 24 hours, filter, Discard the preci...

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Abstract

The invention discloses a medicament composition for treating leukemia and a preparation method of the medicament composition. The composition is obtained by distilling lancea tibetica and dracocephalum tanguticum, collecting volatile oil to prepare a volatile oil clathrate compound, concentrating and drying filtrate and an ethanol extracting solution of anemone rivularis and ranunculus brotherusii to obtain an extract, and then uniformly mixing the volatile oil clathrate compound with the extract. According to the medicament composition disclosed by the invention, the survival time of a mice serving as a leukemia model can be obviously prolonged, so that the proportion of a T cell subset of the model mice tends to be normal, the secretion level of cell factors such as IL-2, IL-6 and IFN-gamma is increased, and the immunosuppression caused by tumors is recovered. The medicament composition can still improve the activity of naturally killing cells (NKC) and interleukin-2(IL-2) of the leukemia mice P388 which is subjected to chemotherapy, and the immunosuppression caused by the chemotherapy is gradually recovered; therefore the medicament composition has a positive significance for treatment of leukemia, reconstruction of the immunity and prevention of the immunosuppression caused by the tumor chemotherapy.

Description

technical field [0001] The invention relates to a pharmaceutical composition for treating leukemia and a preparation method thereof, belonging to the technical field of medicine. Background technique [0002] Leukemia is a malignant clonal disease of hematopoietic stem cells. Clinically, different degrees of anemia, hemorrhage, infection, fever, enlargement of liver, spleen, lymph nodes and bone pain can be seen. According to reports, the incidence of leukemia in various regions of my country occupies the sixth place among various tumors. Because leukemia cells originate from the blood system and have the characteristics of tumor cells, it is customarily called blood cancer. The distribution of leukemia is worldwide, accounting for about 5% of the total incidence of cancer, most of which are children and young people. The number of leukemia patients in my country is about 3-4 per 100,000 population, and there are more men than women. Leukemia ranks 6th (male) and 8th (fe...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/71A61P35/02
Inventor 王彦厚董芳王嘉南
Owner 淄博市食品药品检验所
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