Method for producing lactic acid

A kind of manufacturing method, the technology of lactic acid, is applied in the manufacture field of lactic acid, can solve the problem such as the unclear method that the LDH activity of Rhizopus genus is improved

Inactive Publication Date: 2014-12-24
KAO CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the method for improving the LDH activity of Rhizopus without relying on gene recombination is still unclear.

Method used

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  • Method for producing lactic acid
  • Method for producing lactic acid
  • Method for producing lactic acid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0090] Examples are shown below to describe the present invention more specifically.

[0091] The preparation method of manufacturing example 1 spore suspension

[0092] Rhizopus oryzae ( Rhizopus oryzae) JCM14625 strain (=NBRC 5384) spore suspension freeze-preserved sample (-80°C) was inoculated with 1 platinum loop in PDA medium (Difco Potato Dextrose Agar, manufactured by Becton, Dickinson and Company), and cultured statically at 30°C 7 to 10 days. After visually confirming the growth of mycelia and the blackening of the ends of mycelium accompanied by sporulation, 30 to 40 mL of physiological saline was added thereto, and the mycelium and spores were transferred together using a platinum ring to a 50-mL capped centrifuge tube (manufactured by Greiner ), mixed vigorously in the tube. The mixed spore suspension was filtered through a 3GP100 cylindrical funnel-shaped glass filter (manufactured by Shibata Scientific Co., Ltd.) to obtain a spore liquid. The number of spo...

manufacture example 2

[0093] Preparation of Manufacturing Example 2 Mycelia

[0094] To a baffled Erlenmeyer flask (manufactured by Asahi Glass Co., Ltd.) with a volume of 200 mL, 80 mL of PDB medium was added without adding the surfactant described below or at a final concentration of 0.5% (w / v). The Rhizopus oryzae prepared in 1 ( Rhizopus oryzae ) spore suspension to become 1×10 3 Each-spore / mL-medium was inoculated, and then cultured with agitation at 170 rpm for 3 days at 27°C. Since the mycelia remained on the filter, the above-mentioned culture was filtered through a pre-sterilized stainless steel sieve with a mesh size of 250 μm (manufactured by As One), and the bacterial cells were collected on the filter.

[0095] Surfactant

[0096] Sorbitan monolaurate: RHEODOL (registered trademark) SP-L10 (Kao)

[0097] Sorbitan monooleate: RHEODOL (registered trademark) SP-O10V (Kao)

[0098] Polyoxyethylene (3) lauryl ether: EMULGEN (registered trademark) 103 (Kao)

[0099] Polyoxyethylene ...

manufacture example 3

[0108] Proliferation of production example 3 mycelium

[0109] 100mL of inorganic culture solution (composition: 10% glucose, 0.1% ammonium sulfate, 0.06% potassium dihydrogen phosphate, 0.025% magnesium sulfate heptahydrate, 0.009% zinc sulfate heptahydrate, 3.0-4.0 g (wet weight) of recovered bacteria were inoculated in 5.0% calcium carbonate (all concentrations are % (w / v)), and cultured with stirring at 220 rpm for about 40 hours at 27°C. Next, the culture cultured with the above-mentioned inorganic culture solution was filtered with a stainless steel mesh filter holder (manufactured by MILLIPORE) sterilized in advance, and bacterial cells were collected on the filter. Further, the bacterial cells were washed with 100 mL of physiological saline on the filter holder. Physiological saline used for washing was removed by suction filtration. The obtained bacterial cells were used for the following LDH activity evaluation and lactic acid fermentation productivity evaluation. ...

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Abstract

Provided are: a bacterium belonging to the genus Rhizopus, which has an improved lactic acid production ability; and a method for producing lactic acid using the bacterium. A method for improving the activity of lactate dehydrogenase in a bacterium belonging to the genus Rhizopus, comprising germinating a spore of the bacterium belonging to the genus Rhizopus in a culture solution containing a surfactant under specified conditions to produce a mycelium of the bacterium; and a method for producing lactic acid using the mycelium of the bacterium belonging to the genus Rhizopus.

Description

technical field [0001] The invention relates to a method for producing lactic acid. Background technique [0002] Lactic acid, which is a raw material of poly-lactic acid (PLA), attracts attention as a biodegradable plastic material, is obtained from a plant-derived raw material by a biological production method. Therefore, PLA is known to not increase atmospheric CO 2 amount of material (carbon neutral material). There are D-body and L-body of optical isomers in lactic acid, and in order to control the characteristics of polylactic acid as a plastic material, lactic acid with high optical purity is required. [0003] Conventionally, Lactobacillus ( Lactobacillus ), Lactococcus ( Lactococcus ) and other lactic acid bacteria are cultured in an anaerobic environment to make lactic acid fermentation (non-patent document 1). Further, as a method for specifically producing L-lactic acid, it is known to cultivate Rhizopus ( Rhizopus ) methods (Patent Documents 1 to 3). Al...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12P7/56C12R1/845
CPCC12P7/56C12N1/14
Inventor 壶井雄一高桥史员西村祐美泽田和久
Owner KAO CORP
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