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Preparation and application of sika deer spleen extract having function of increasing white blood cells

A technology for increasing white blood cells and sika deer, applied in the direction of extracellular fluid diseases, medical preparations containing active ingredients, applications, etc., to achieve the effect of increasing content and activity

Active Publication Date: 2015-01-28
北京中京丰创科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Leukopenia is clinically treated with hormones and lithium salts, although the curative effect is improved, but the side effects are large

Method used

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  • Preparation and application of sika deer spleen extract having function of increasing white blood cells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] . Weigh 250g of sika deer spleen and remove the surface connective tissue;

[0021] . Add 750ml of normal saline and grind for 5 minutes with a colloid mill;

[0022] .Heat the deer spleen suspension to 50°C, adjust the pH value to 5, add 0.25g neutral protease, 0.75g trypsin and 1.5g 5'-phosphodiesterase at the same time to incubate for 2 hours, after the incubation is completed, under 4000g centrifugal force Carry out centrifugation, obtain supernatant liquid I;

[0023] .The deer spleen precipitate obtained in the previous step continued to be added with an equal mass of normal saline, and the temperature was raised to 60°C. At the same time, 0.12g of neutral protease, 0.48g of papain and 0.6g of 5'-phosphodiesterase were added to incubate for 3 hours, and then in Centrifuge under 4000g centrifugal force for 10min to obtain supernatant II liquid;

[0024] e. Mix the supernatant liquid I and liquid II, then perform ultrafiltration with a 10KD ultrafiltration...

Embodiment 2

[0026] . Weigh 250g of sika deer spleen and remove the surface connective tissue;

[0027] . Add 1200ml of normal saline, and grind for 5 minutes with a colloid mill;

[0028] .Heat the deer spleen suspension to 55°C, adjust the pH value to 5, add 0.51g neutral protease, 1.48g trypsin and 3.1g 5'-phosphodiesterase at the same time and keep it warm for 2 hours. centrifuged at a lower temperature to obtain the supernatant liquid I;

[0029] .The deer spleen precipitate obtained in the previous step was continued to be added with an equal mass of normal saline, and the temperature was raised to 60°C. At the same time, 0.22g of neutral protease, 0.78g of papain and 1.05g of 5'-phosphodiesterase were added to incubate for 1h, and then in Centrifuge under 4000g centrifugal force for 10min to obtain supernatant II liquid;

[0030] e. Mix the supernatant liquid I and liquid II, then perform ultrafiltration with a 10KD ultrafiltration membrane, and spray-dry the ultrafiltrate...

Embodiment 3

[0032] . Weigh 250g of sika deer spleen and remove the surface connective tissue;

[0033] . Add 1400ml of normal saline and grind for 4 minutes with a colloid mill;

[0034] .Heat the deer spleen suspension to 50°C, adjust the pH value to 5, add 0.54g neutral protease, 1.62g trypsin and 3.52g 5'-phosphodiesterase at the same time and incubate for 1h. After the incubation, under 4000g centrifugal force Carry out centrifugation, obtain supernatant liquid I;

[0035] .The deer spleen precipitate obtained in the previous step was continued to be added with an equal mass of normal saline, and the temperature was raised to 60°C. At the same time, 0.28g of neutral protease, 0.89g of papain and 1.45g of 5'-phosphodiesterase were added to incubate for 2h, and then in Centrifuge under 4000g centrifugal force for 10min to obtain supernatant II liquid;

[0036] e. Mix the supernatant liquid I and liquid II, then perform ultrafiltration with a 10KD ultrafiltration membrane, and ...

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Abstract

The invention discloses a novel extracting method of deer spleen extract and an application of deer spleen extract in immunity enhancement drugs. A traditional deer spleen extracting technology is improved, and centrifugation sediments are further extracted by a complex enzyme. Both polypeptide content and nucleotide content are improved by 30%. Moreover, the inosine ingredient occupies 8% of total nucleotide content in the extract, and the inosine content in the extract is improved. Meanwhile, the activity of the extract obtained by the method is improved by 20% than that of transfer factors extracted by the original technology for increasing the white blood cells.

Description

Technical field: [0001] The invention discloses the preparation and application of a sika deer spleen extract which increases the effect of white blood cells, and belongs to the technical field of biopharmaceuticals. technical background: [0002] Leukopenia is very common clinically, mostly secondary. When the white blood cells decrease, the resistance will also decrease, and symptoms such as dizziness, fatigue, heart palpitations, low fever, listlessness, insomnia, loss of appetite, pharyngitis, and mucosal ulcers will occur, seriously affecting people's quality of life and health. Leukopenia is treated clinically with hormones and lithium salts. Although the curative effect is improved, the side effects are large. For a health care product for treating leukopenia with good curative effect, low cost and no side effects, the market demand is huge. [0003] Transfer factor is a complex composed of polypeptides and oligonucleotides, which has strong chemotactic activity o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/26A61P7/00
CPCA23V2002/00A61K35/26A23V2200/324A23V2250/204
Inventor 逯家辉王迪刘洋彭小君滕乐生王贞佐滕利荣孟庆繁刘艳贾东旭周毓麟程瑛琨
Owner 北京中京丰创科技有限公司
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