A strain of Raoult ornithinolyticum and its application

A technology of ornithine and bacterial agent, applied in Raoule ornithine solubilization and its application field, can solve the problem that the effect of promoting growth and improving soil is not satisfactory, and the phosphate solubilizing bacteria cannot play a good role and solve the problem. The effect of phosphorus inoculants is getting worse and other problems, so as to achieve the effect of promoting phosphorus utilization, good phosphorus solubilization ability, and improving traits.

Inactive Publication Date: 2016-11-23
HENAN UNIVERSITY OF TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Scientists have also been working on developing better phosphate solubilizing bacteria to improve the current bad situation. However, because the cultivation conditions of phosphate solubilizing bacteria in the laboratory are too different from the environmental conditions of the soil in practical applications, most of them Phosphorus solubilizers are still unsatisfactory in promoting growth and improving soil
An important reason for the poor effect of phosphate solubilizing bacteria is that there are too many nutrients in the medium, and phosphate solubilizing bacteria cannot function well under oligotrophic conditions.

Method used

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  • A strain of Raoult ornithinolyticum and its application
  • A strain of Raoult ornithinolyticum and its application
  • A strain of Raoult ornithinolyticum and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1. Isolation and identification of Raoultella ornithinolytica WN-3

[0030] 1. Isolation of strains

[0031] In April 2012, the activated sludge of Wulongkou Sewage Treatment Plant in Zhengzhou City, Henan Province was collected. On the way, the sample was placed in an ice box below 4°C, and after being brought back to the laboratory, 5g of the sample was weighed and placed in a triangle filled with 45mL of sterile water. Shake the bottle at 28°C and 150rpm for 1h. After the sample is evenly dispersed in sterile water, draw 1mL of the suspension into a test tube filled with 9mL of sterile water, mix well, and make the concentrations of 10 -1 -10 -6 g / mL bacterial suspension, draw 100 μL from each concentration of bacterial suspension and spread it on 1 / 4 Montina solid medium plate, and cultivate it in a constant temperature incubator at 28°C for 5-6 days. Different colonies with transparent circles on the edge of the colonies were streaked and purified, and ma...

Embodiment 2

[0039] Embodiment 2, the phosphorus-solubilizing ability of Raoultella ornithinolytica (Raoultella ornithinolytica) WN-3

[0040]1. Inoculate one loop of WN-3 colony preserved on the slant into 5ml LB liquid medium, and cultivate overnight at 28°C on a 150rpm shaker to obtain bacterial solution 1, and then add 5ml of bacterial solution 1 to 100ml LB liquid medium , cultured on a shaker at 150 rpm at 28°C for 24 hours to obtain bacterial solution 2, take 5ml of bacterial solution 2, centrifuge (8000rpm, 10min), wash twice with sterile water, and resuspend with sterile water to obtain bacterial solution 3.

[0041] 2. Put the bacterial solution 3 into 50ml 1 / 4 Montina liquid medium, culture at 28°C, shaker at 150rpm for 3 days, during which 5ml samples were taken at 0, 0.5, 1, 1.5, 2 and 3 days, and centrifuged at 8000rpm After 10 minutes, the supernatant was taken to measure the concentration of soluble phosphorus, and the phosphorus-solubilizing ability of the strain WN-3 chan...

Embodiment 3

[0044] Embodiment 3, the growth-promoting experiment of Raoultella ornithinolytica (Raoultella ornithinolytica) WN-3 to potted wheat

[0045] 1. The activation of the bacterial strain is the same as step 1 of Example 2, until the bacterial liquid 2 is obtained, and it is centrifuged (6000rpm, 10min) to obtain the bacterium of WN-3 with a wet weight of 0.1g, and 3ml of distilled water is added to resuspend the bacterium to prepare Bacteria.

[0046] 2. Set up the following control group and experimental group:

[0047] Experimental group: Water the flowerpot thoroughly first, then pour the inoculum prepared in step 1 into the center of the flowerpot, and evenly sprinkle 1 gram (about 24 grains) of wheat (Jimai No. 38) on the place where the inoculum is poured. Cover with damp soil.

[0048] Control group: no bacterial agent, other steps are the same as the experimental group.

[0049] Cultivate the experimental group and the control group in the greenhouse at 25°C for 20 day...

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Abstract

The invention discloses a strain of Raoultella ornithinolyticum and its application. The invention discloses a strain of Raoultella ornithinolytica, the preservation number of which is CGMCC No.9407. The Raoultella ornithinolytica WN‑3 disclosed by the present invention with a preservation number of CGMCC No.9407 is tolerant to an oligotrophic living environment and has good phosphorus-solubilizing ability, which can promote the improvement of soil phosphorus utilization rate. Raising, increasing crop yield and / or improving its traits, improving farmland soil, has significant economic, environmental and social benefits.

Description

technical field [0001] The invention relates to a Raoultella ornithinolyticum strain and its application, belonging to the field of biotechnology. Background technique [0002] More than 70% of the cultivated land soil in my country is in a state of phosphorus deficiency. Although the potential phosphorus content in most farmland soils is large after long-term application of phosphorus fertilizers, the amount of phosphorus that can be used by crop growth and development is very small, and 95% of the soil All of the above phosphorus gradually accumulates in insoluble and ineffective forms. Long-term low-efficiency use of chemical phosphate fertilizers will cause changes in soil physical and chemical properties, resulting in long-term adverse effects. Studies have found that there is a type of microorganisms that can convert insoluble phosphorus, which is difficult for plants to absorb and utilize, into soluble phosphorus forms that are easy to use, promote the absorption of p...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/00A01P21/00C05G3/00C12R1/01
CPCA01N63/00C05G3/00C12N1/205C12R2001/01
Inventor 李海峰李志建屈建航赵凤霞李向阳
Owner HENAN UNIVERSITY OF TECHNOLOGY
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