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Method for preparing rare ginsenoside Rg2 through biotransformation

A kind of ginsenoside, a rare technology, applied in the field of biochemical pharmaceuticals, can solve the problems of polluting the environment, many by-products, large organic solvents, etc., and achieve the effects of high yield, easy operation and low cost

Inactive Publication Date: 2015-02-11
NORTHEAST NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the chemical method has low yield, many by-products, consumes a lot of organic solvents, and pollutes the environment, while the biotransformation method using microorganisms can overcome these disadvantages, so the biotransformation method is regarded as the most potential way to prepare rare ginsenosides method

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Embodiment 1——preparation of fermentation strain

[0020] Cellulosimerbium sp.21 stored in freeze-dried tubes was streaked and inoculated on LB solid medium, and cultured in a constant temperature incubator at 37°C for 1 day to activate the strains. The activated strains were inoculated in LB liquid medium, and cultured overnight in a horizontal shaker at 37°C and 180 rpm to obtain seed liquid.

Embodiment 2

[0022] Add ginseng stem and leaf total saponins with a final concentration of 6 mg per ml and 3% seed solution to 15 ml of LB medium, and place them in a horizontal shaker at 37°C for cultivation at a speed of 180 rpm. After culturing for 12 hours, take it out, add n-butanol, extract ginsenosides, and detect with TLC method, the ginsenoside Re in the conversion product has not been completely converted, and there is a small amount of residue.

Embodiment 3

[0024] Add ginseng stem and leaf total saponins with a final concentration of 6 mg / ml and a seed solution with a volume fraction of 3% to 15 ml of LB medium, and culture in a horizontal shaker at 37°C with a rotational speed of 180 rpm. After culturing for 24 hours, take it out, add n-butanol, extract ginsenosides, and detect by TLC method. The ginsenoside Re in the conversion product is completely converted to produce rare ginsenoside Rg2, a small amount of ginsenoside Rh1 and triol aglycone. The transformed product was separated by silica gel column chromatography, and the proportion of transformed ginsenoside Rg2 in the transformed product reached 42%.

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Abstract

The invention relates to a method for preparing rare ginsenoside Rg2. According to the method, Cellulosimicrobium sp.21 serves as fermented culture biotransformation total saponins of panax ginseng. By means of the method, the high-quality rare ginsenoside Rg2 can be efficiently prepared.

Description

technical field [0001] The invention relates to a preparation method of rare ginsenoside Rg2, mainly relates to a method for preparing rare ginsenoside Rg2 by using a cellulosimicrobium sp.21 as a fermentation strain to biotransform total saponins of ginseng stems and leaves, and belongs to the field of biochemical pharmacy. Background technique [0002] Ginsenoside (ginsenoside) is one of the main active ingredients in ginseng, which is composed of saponin and sugar side chains, and is a sterol compound. According to the different glycoside structure, ginsenosides can be divided into two categories: oleanane-type pentacyclic triterpenoid saponins (oleanolic acid saponins) and dammarane-type tetracyclic triterpene saponins (dammarane), the latter of which It can be divided into protopanaxadiol saponins and protopanaxatriol saponins. Ginsenosides have strong pharmacological activity and play an important role in anti-tumor, anti-fatigue, anti-hypoxia, inhibition of myocardia...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12P33/20C12R1/01
CPCC12P33/20C12N1/205C12R2001/01
Inventor 周义发高娟王楠王姣孙成新台桂花
Owner NORTHEAST NORMAL UNIVERSITY
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