Preparation and application for growth-promoting formula with bamboo vinegar replacing forage antibiotic

A technology of bamboo vinegar liquid and antibiotics, applied in the field of livestock and poultry feed, can solve problems such as differences in bacteriostatic and bactericidal effects, and achieve the effects of protecting the ecological environment, short growth cycle, and easy sustainable production.

Inactive Publication Date: 2015-02-25
JIANGYIN CHINA GRAND BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Bamboo vinegar has strong antibacterial and bactericidal effects on bacteria and fungi, but there are certain differences in antibacterial and bactericidal effects, and the order of inhibitory effects is mold > bacteria > yeast

Method used

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  • Preparation and application for growth-promoting formula with bamboo vinegar replacing forage antibiotic
  • Preparation and application for growth-promoting formula with bamboo vinegar replacing forage antibiotic
  • Preparation and application for growth-promoting formula with bamboo vinegar replacing forage antibiotic

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Escherichia coli and Staphyloccocus aureus were provided by the School of Veterinary Medicine, Nanjing Agricultural University. The bacterial types of Escherichia coli were 452 and 048, and the bacterial types of Staphylococcus aureus were 563 and 187.

[0021] Four kinds of Escherichia coli and Staphylococcus aureus were transferred to LB medium (50μL bacterial solution + 5mL LB medium), cultured on a shaker for 5 hours, distributed into 2mL tubes, centrifuged at 5000g for 4 minutes, eluted to remove Replace the LB medium with new medium. Use a microplate reader to measure the OD600 value, calculate the number of bacteria in the bacterial solution, and then dilute to 1×10 with LB medium 8 CFU / mL.

[0022] According to antibiotic use standards and its recommended dosage in broiler production, namely aureomycin 50-100mg / kg, flavomycin 1-5mg / kg, bacitracin zinc 25-50mg / kg, colistin sulfate 2-20mg / kg , Dilute the 4 feed antibiotics to 2 times the recommended maximum dos...

Embodiment 2

[0031] A kind of bamboo vinegar liquid provided by the present invention replaces the weight percent of each component in the antibiotic growth-promoting formula of feed: 55.1% of corn, 32.2% of soybean meal, 4.5% of corn gluten powder, 3.2% of soybean oil, 5% of premix, Bamboo vinegar 1%, chlortetracycline; later diet: 60.8% corn, 23.7% soybean meal, 5.5% corn gluten powder, 5% soybean oil, 5% premix, 1% bamboo vinegar, chlortetracycline. Among them, the compound premix was purchased from Nanjing Huamu Animal Science and Technology Research Institute of Jiangsu Province, and the bamboo vinegar was purchased from Jiangyin Zhongju Biotechnology Co., Ltd.

[0032] The present invention proposes a kind of method that bamboo vinegar liquid replaces feed antibiotic growth-promoting formula to make compound feed according to the following steps

[0033] A, take by weighing above-mentioned various feed raw materials according to the requirement of each component weight percent in the...

Embodiment 3

[0038] Using a single factor completely randomized design experiment, 240 one-day-old healthy AA broilers were randomly divided into 5 treatment groups. The first group was supplemented with 25 mg / kg aureomycin on the basis of the basal diet, and the last four groups were treated with basal diet respectively. Add feed antibiotics (chlortetracycline) 7.5mg / kg, 12.5mg / kg, 17.5mg / kg, 25mg / kg on the basis of the optimum addition amount (1%) determined in middle addition test 1. There were 6 replicates for each treatment, and 8 chickens per replicate. The basal diet was formulated according to the NRC1994 standard. The test period was 42 days. The broiler chickens were fed with the early diet at the age of 0-21 days, and the broiler chickens were fed with the later diet at the age of 22-42 days.

[0039] Before entering the chicks, the chicken house is thoroughly cleaned and disinfected. The test chickens were reared in cages, with 24h light (natural light during the day and incan...

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Abstract

The invention relates to preparation and application for a growth-promoting formula with bamboo vinegar replacing a forage antibiotic. The formula is characterized in that the formula comprises the following nutrient levels: daily ration (earlier stage/later stage) comprises 20-23%/19.41% of crude protein, 12.56MJ/kg/13.34MJ/kg of metabolic energy, 0.97%/0.81% of calcium, 1.18%/0.81% of lysine and 0.5%/0.81% of methionine; and the nutrient levels in the above mixed formula can be obtained by proportioning the following raw materials according to the following usage amount (earlier stage/later stage): 55.1%/60.8% of corn, 32.2%/23.7% of bean meal, 4.5%/5.5% of corn protein powder, 3.2%/5% of bean oil, 5% of a premix, 1% of bamboo vinegar, and 7.5-25mg/kg of aureomycin. The growth-promoting formula in which the bamboo vinegar is utilized for replacing forage antibiotic has extremely good effect for replacing antibiotic, improving production performance and reducing ammonia gas in faeces.

Description

technical field [0001] The invention belongs to the field of livestock and poultry feed, and relates to the preparation and application of a feed formula added with bamboo vinegar instead of feed antibiotics. Background technique [0002] Since the discovery of antibiotics, many diseases caused by microorganisms are no longer a concern for people, and medical technology has also taken a huge leap forward. Antibiotics have so many benefits that people not only use them to treat diseases, but also use them as feed additives to prevent animal diseases and promote animal growth. The growth-promoting mechanism of antibiotics has been thoroughly studied as early as the mid-1980s, mainly including the following four mechanisms: 1. Inhibit asymptomatic infection; 2. Reduce the utilization of nutrients by microorganisms; 3. Thin the intestinal wall of animals and increase animal resistance Absorption and utilization of nutrients; 4. Reduce the metabolites of harmful microorganisms. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K1/14A23K1/17A23K1/18A23K20/195
Inventor 李春梅崔宇普少瑕李延森
Owner JIANGYIN CHINA GRAND BIOTECH
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