A processing method for improving gel performance of shrimp meatballs
A processing method, the technology of shrimp meatballs, which is applied in the field of food processing, can solve the problems of lack of inherent umami taste of aquatic products, insufficient gel strength of shrimp meatballs, loss of water-soluble protein, etc., shorten the thawing time and make the process parameters easy to master , the effect of improving the performance of the gel
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Embodiment 1
[0025] Example 1: (preparation of shrimp head protein hydrolyzate)
[0026] Fresh or frozen shrimp heads, boiled at 90°C for 30 minutes, crushed with a tissue grinder, homogenized with water, adjusted to pH 8.0 and preheated to 50°C, added Alcalase protease (enzyme activity 100kU / g, Novozymes, Denmark) ) 0.3% (w / w) enzymatic hydrolysis at 50°C for 2h, and then inactivate the enzyme at 90°C for 30min, then lower the temperature to 60°C and adjust the pH to 7.5, add Flavourzyme protease (500MG, Novozymes, Denmark) 0.1% (w / w) Enzyme hydrolysis at 60°C for 4 hours, and then inactivate the enzyme at 90°C for 30 minutes; the hydrolyzate was centrifuged at 6000rpm to remove the precipitate, the supernatant was incubated at 80°C for 10min, and then the fat layer was removed by centrifugation at 8000rpm, followed by electrodialysis to remove salt, Ultrafiltration membrane ultrafiltration (molecular weight cut-off: 30kDa), concentrated by rotary evaporator and then freeze-dried, the cr...
Embodiment 2
[0028] Put frozen shrimps into plastic cages (cage height ≤ 20cm), the height of the material should not exceed the cage, and then place the cage in a continuous radio frequency thawing machine (the distance between the two electrode plates of the radio frequency thawing machine is 24.5cm, the radio frequency is 27.12MHz, On the conveyor belt with a working power of 7.5kW, turn on the radio frequency thawing machine and thaw for 12~15min. Take 10kg of thawed shrimp, put it in a meat grinder and chop and mix for 1~2min, then add 200g of salt, 300g of glucose and 700g of shrimp head protein hydrolyzate, continue chopping and mixing for 3~5min until the shrimp meat is milky white and sticky. Entering Leuconostoc enterica into the minced shrimp ( Leuconostoc mesenteroides , ATCC8086) bacterial suspension (containing 10 8 ~10 9 CFU / mL), the inoculum size was 2%, mixed evenly, put into a closed stainless steel container and evacuated, and anaerobically fermented at 6°C for 24h. ...
Embodiment 3
[0030] Put frozen shrimps into plastic cages (cage height ≤ 20cm), the height of the material should not exceed the cage, and then place the cage in a continuous radio frequency thawing machine (the distance between the two electrode plates of the radio frequency thawing machine is 24.5cm, the radio frequency is 27.12MHz, On the conveyor belt with a working power of 7.5kW, turn on the radio frequency thawing machine and thaw for 12~15min. Take 10kg of thawed shrimp, put it in a meat grinder and chop and mix for 1~2min, then add 300g of salt, 500g of glucose and 500g of shrimp head protein hydrolyzate in turn, continue chopping and mixing for 3~5min until the minced shrimp is milky white and sticky. Introduce Lactobacillus flexus ( Lactobacillus curvatus , ATCC51436) bacterial suspension (containing 10 8 ~10 9 CFU / mL), the inoculum size was 3.5%, mixed evenly, placed in a closed stainless steel container to vacuumize, and anaerobically fermented at 8°C for 18h. The fermente...
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