Biological enrichment method of conjugated linolenic acid isomer

A conjugated linolenic acid and bio-enrichment technology, applied in the field of biomedicine, can solve problems such as long fermentation cycle, achieve the effects of enhancing health care function, improving enrichment level and overcoming influence

Active Publication Date: 2015-04-01
NANCHANG UNIV
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

However, the output of conjugated linolenic acid reported in the literature is usually less than 1mg/mL, and there is a shortcoming of a long fermentation period.
Invention patent CN200410060670.9 reports specific biosynthesis by Lactobacillus casei CGMCC 1.574 c 9, t 11, c The method of 15-conjugated linolenic acid isomers, although the product has high purity, the yield in MRS and skimmed milk, a common medium for lactic acid bacteri

Method used

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  • Biological enrichment method of conjugated linolenic acid isomer
  • Biological enrichment method of conjugated linolenic acid isomer

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Experimental program
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Effect test

Embodiment 1

[0020] After soaking 1 part of low-temperature defatted soybeans with 4 parts of water for 6 hours, coarsely grind and then finely grind once with a refiner, and then process twice with a colloid mill to obtain a mixed emulsion, after boiling, adjust the protein content in it to The mass percentage is 8% to obtain the basal medium. Add 1% fructo-oligosaccharides by mass to the basic medium, and pasteurize to obtain a bio-enriched medium. Insert 5% Lactobacillus casei ( Lactobacillus casei ) CGMCC 1.574 production strain, and at the same time add 0.4% linolenic acid substrate, mix well and incubate at 37°C for 24h. After enrichment by this method, the product has a characteristic absorption peak at 268nm, indicating that the product is conjugated linolenic acid, and further confirmed by capillary electrophoresis analysis that the isomer is c 9, t 11, c 15-conjugated linolenic acid isomers. The concentration of the substrate linolenic acid has a significant impact on the sy...

Embodiment 2

[0022] After soaking 1 part of low-temperature defatted soybeans with 4 parts of water overnight, coarsely grind and then finely grind 1 time with a refiner, and then process 2 times with a colloid mill to obtain a mixed emulsion. After boiling, adjust the protein content therein to The mass percentage is 5%, and the skimmed milk powder with a mass percentage of 3% is added to obtain a basal medium. Add 1% fructo-oligosaccharides by mass to the basic medium, and pasteurize to obtain a bio-enriched medium. Insert 5% Lactobacillus casei ( Lactobacillus casei ) CGMCC 1.574 strains for production, at the same time add 0.4% by mass percentage of linolenic acid substrate, mix well and incubate at 37°C for 24h, the product c 9, t 11, c The content of 15-conjugated linolenic acid isomers is 0.38% by mass. After the fermentation product is spray-dried or freeze-dried at low temperature, it is processed into tablets by pressing and coating, which can effectively prevent c 9, t 11,...

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Abstract

The invention relates to a biological enrichment method of a conjugated linolenic acid isomer. The biological enrichment method comprises biological enrichment culture medium preparation, production strain preparation, biological enrichment of the conjugated linolenic acid isomer, and other steps to obtain the c9,t11,c15-conjugated linolenic acid isomer. According to the present invention, the c9,t11,c15-conjugated linolenic acid isomer mass content in the obtained product is more than 0.36%, and the substrate conversion rate is more than 90%; and with the biological enrichment culture, the c9,t11,c15-conjugated linolenic acid isomer-rich anti-cancer function product is obtained, proteins and cellulose in defatted soybean can be decomposed into polypeptides, soluble dietary fibers and other active factors having health functions through lactic acid bacteria, and the health function of the product is significantly enhanced so as to further broaden the application range of the product.

Description

technical field [0001] The invention belongs to the technical field of biomedicine. Background technique [0002] Conjugated linolenic acid is a conjugated isomer of linolenic acid, which is a collective name for a group of octadecyl-conjugated trienoic acids. There are many positional isomers and geometric isomers, such as: c 9, t 11, c 15-conjugated linolenic acid and t 10, c 12, c 15-conjugated linolenic acid isomers, etc. Studies have shown that conjugated linolenic acid has physiological functions such as anti-cancer, prevention of atherosclerosis, and weight loss, and its physiological activities are isomer-specific, such as: c 9, t 11, c 15-conjugated linolenic acid isomers have anticancer and atherosclerotic effects. In nature, conjugated linolenic acid mainly exists in the milk and fat of ruminants, mainly composed of c 9, t 11, c 15-conjugated linolenic acid and a small amount t 9, t 11, c 15-conjugated linolenic acid and other isomers, but its conte...

Claims

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Application Information

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IPC IPC(8): C12P7/64C12R1/245
CPCC12P7/6427
Inventor 刘晓华李海星
Owner NANCHANG UNIV
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