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Noninvasive fabricating method for pulmonary fibrosis animal model

A technology of animal model and pulmonary fibrosis, which is applied in medical science, veterinary equipment, animal husbandry, etc., can solve the problems of uneven dosage, difficulty in calculation, mastering the amount of inhalation, and many device requirements, etc. Simple, reduce exogenous damage, and reduce the cost of modeling

Inactive Publication Date: 2015-05-13
天津中医药大学第二附属医院
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  • Application Information

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Problems solved by technology

Therefore, in addition to the atomized particle size, there are more requirements for the device, and it is difficult to calculate and grasp the amount of inhalation, which may easily cause uneven dosage.

Method used

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  • Noninvasive fabricating method for pulmonary fibrosis animal model
  • Noninvasive fabricating method for pulmonary fibrosis animal model
  • Noninvasive fabricating method for pulmonary fibrosis animal model

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Embodiment Construction

[0022] The technical solutions of the present invention will be further described in detail below in conjunction with the accompanying drawings and specific embodiments.

[0023] Experimental animals: 60 clean-grade Wistar male rats, aged 3-6 months, weighing 180 ± 20g, provided by Beijing Huafukang Biotechnology Co., Ltd., animal qualification (license) certificate number: SCXK (Beijing) 2009 -0004; experimental rats were fed in Tianjin Experimental Animal Center, the relative humidity of the feeding environment was 50%-60%, and the temperature was controlled at 18-22°C; conventional feed, free drinking water, regular ventilation and flushing of feces, keeping the environment quiet, The experiment started after 1 week of adaptive feeding.

[0024] Experimental drug: bleomycin (Qishu mixture group, prednisone group) produced by Nippon Kayaku Co., Ltd., import license number X20000349

[0025] Modeling method

[0026] The method of instilling bacterial solution in the trachea...

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Abstract

The invention discloses a noninvasive fabricating method for a pulmonary fibrosis animal model. The method comprises the steps: the method that the bacterium solution dropping is poured inside a lower trachea through an endoscope is adopted, and a nasal zero degree 4 # hard-type endoscope, a xenon lamp cold light source and a Panasonic KS-822 video monitoring system are utilized; a rat is placed in a dryer with 26 cm diameter, and the ether anesthesia is carried out on the rat, the 200 microlitre of the medicine bleomycin is ejected inside the trachea, the medicine is fully flowed into the bronchus along with the airflow when inhaling air, elastic fixings clamped on the four limbs of the rat are removed, the rat is placed into a rat cage to wait for the anabiosis, the anabiosis period ranges from 30 sec to 60 sec, and the rat is placed into a constant temperature animal house for feeding; the activity, the feeding and the spirit conditions of the inoculated rat are observed every day, and the corresponding data is recorded.

Description

technical field [0001] The invention belongs to the technical field of animal experimental model construction, and in particular relates to a non-invasive method for making an animal model of pulmonary fibrosis. Background technique [0002] There are many existing methods for constructing animal models of pulmonary fibrosis, mainly including: tracheotomy, tracheal intubation, forehead mirror-guided perfusion, intraperitoneal injection or intravenous infusion of chemical drugs, radiation, oropharyngeal drug delivery . [0003] There are many modeling methods, but each has a fly in the ointment, and there are more or less shortcomings. The exogenous trauma of tracheotomy on the specimen mouse is complicated and has a great impact on relevant laboratory data, and the operation is time-consuming; the method of tracheal intubation or frontal mirror guidance has large blindness in operation, poor accuracy, and low success rate. Low, time-consuming and other defects. [0004] T...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61D7/00
CPCA61D7/00A01K67/027A01K2267/03
Inventor 孙增涛郑兆晔廉富杨晓敏闫博刘恩顺祁海燕李美凤封继宏
Owner 天津中医药大学第二附属医院
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