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Application of chlorothiazide in preparing medicament for inhibiting tumor cell metastasis and diffusion

A tumor cell, chlorothiazide technology, applied in the application field of medicine, can solve the problems such as the pharmacological effects of chlorothiazide have not been found, and achieve the effect of reducing the research and development cycle and reducing the number of deaths

Inactive Publication Date: 2015-05-13
南京格耀生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patented technology prevents cancer from getting back into their original place when they start growing again after being treated with certain medications or treatments like chemotherapy. It works because it blocks how many healthy ones have gone through this process instead of killing them themselves completely. Additionally, there's no harmful side effect on any older people who were given these chemical agents more than once during previous years.

Problems solved by technology

Technic problem: Chemically synthesized chemical agents called ibramsitylin are used on treating high blood pressures such as heart failure due to excessive sodium retention caused by certain medications like beta blocker therapy. These chemistry produce unwanted side effects which affect patient outcomes including reduced urinary output, decreased kidney function, elevated plasma levels, etc., making it necessary to find other options than just reducing salt content without causing these negative health consequences.

Method used

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  • Application of chlorothiazide in preparing medicament for inhibiting tumor cell metastasis and diffusion
  • Application of chlorothiazide in preparing medicament for inhibiting tumor cell metastasis and diffusion
  • Application of chlorothiazide in preparing medicament for inhibiting tumor cell metastasis and diffusion

Examples

Experimental program
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Effect test

Embodiment 1

[0023] Example 1: Application of chlorothiazide in the preparation of drugs for inhibiting the metastasis and spread of colon cancer cells. The specific experimental plan is as follows:

[0024] 1. Experimental materials

[0025] Colon cancer cells (HT29) were used in the experimental stage of the present invention, and the drug used in the positive control was nocodazole (experimental concentration: 10uM). The negative control group received no treatment.

[0026] Wherein experiment adopts chlorothiazide compound C 7 h 6 ClN 3 o 4 S 2 , CAS NO. is 58-94-6, the concentrations are 10uM, 5uM, 1uM, 0.5uM.

[0027] 2. Experimental method

[0028] For colon cancer cells cultured for 24 hours (cell density: 10 5 ) for drug stimulation for 2 hours:

[0029] (1) Centrifuge the colon cancer cells in a better state in the culture dish, and add an appropriate amount of culture medium to make a cell suspension;

[0030] (2) Get the cell suspension count, according to 10 5 The ...

Embodiment 2

[0038] Example 2: Transwell cell migration assay was used to determine the effect of chlorothiazide on the migration ability of colon cancer cells.

[0039] Will 1×10 5 The HT29 cells were inoculated in Transwell chambers with a 12.0 μm membrane at the bottom, and continued to be cultured. After the cells adhered to the wall, DMEM complete medium with different concentrations of chlorothiazide was added to the upper chamber. To maintain osmotic pressure, 0.05%-0.2% BSA was added, and DMEM complete medium containing 15% FBS was added to the lower chamber for 24 hours. The unmigrated cells on the upper surface of the membrane were carefully wiped off with a cotton swab, and the cells that migrated to the lower surface of the membrane were fixed with 2% PFA at 37C for 10 minutes and then stained with Giemsa. Ten visual fields were randomly selected under a high-power microscope to count the migrated cells.

[0040] Cell migration rate = [the average number of cells in the exper...

Embodiment 3

[0044] Example 3: The damage repair experiment detects the effect of chlorothiazide on the migration ability of tumor cells.

[0045] Take HT29 cells in the logarithmic growth phase, and add 1×10 cells to each well of a 24-well plate 5 After the cells grow into a single layer, draw a "one"-shaped scratch, wash with PBS, add different concentrations of chlorothiazide to the control group and the experimental group, and take pictures. After 22 hours of culture, the same part is taken again to measure the migration distance. The experiment was repeated 3 times, and the cell migration rate=[(0h average value of the experimental group-22h average value of the experimental group) / (0h average value of the blank control group-22h average value of the blank control group)]×100%.

[0046]Chlorthiazide inhibited migration of LTEP and A549 cells at different concentrations (0.5-10 μg / ml) for 22 hours. The results showed that chlorothiazide had a dose-dependent inhibitory effect on colon c...

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Abstract

The invention discloses application of chlorothiazide in preparing a medicament for inhibiting tumor cell metastasis and diffusion. According to the application disclosed by the invention, the medicament prepared by utilizing the chlorothiazide is capable of inhibiting the metastasis ability of tumor cells; as being a low toxic medicament, the chlorothiazide can have large effect of blocking the process of tumor diffusion; meanwhile, other relative tumor medicaments are also adopted to carry out treatment, and the mortality of normal cell can be greatly reduced; while the traditional tumor medicaments are mostly cytotoxic medicaments, a great amount of the normal cells die together while the tumor cells are killed.

Description

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Claims

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Application Information

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Owner 南京格耀生物科技有限公司
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