Culture medium for increasing bacteria specific content in escherichia coli of plasmid DNA (Deoxyribonucleic Acid) content

Abstract

The invention discloses a culture medium for increasing a bacteria specific content in escherichia coli of a plasmid DNA (Deoxyribonucleic Acid) content, relating to the field of biomedical sciences. Each litre of the culture medium contains the following components: 5 g of yeast powder, 5 g of peptone or beef extract, 9 g of sodium chloride, 10 ml of glycerinum, 5 g of lactose, 1 g of citric acid, 10 g of disodium hydrogen phosphate, 0.2 g of sodium hyaluronate, 3 g of ammonium sulphate, and 12 g of calcium chloride. The content of the peptone or beef extract is reduced on the basis of the original LB (Luria-Bertani) culture medium; 9g of sodium chloride is added into 1 L; a cell isotonic culture solution is obtained; calcium chloride is used for reducing bacteria settlement, assisting to stop bacteria flocculation and mutually inhibiting growth; a slight anaerobic environment is set up by introducing a part of CO2 in the culture medium, so that the tolerance of escherichia coli is increased; by means of addition of hyaluronic acid and ammonium sulphate, the membrane permeability is adjusted; diffusion and operation of water electrolytes are kept; and a cell metabolism microenvironment is provided.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to a culture medium for increasing the bacterial ratio of plasmid DNA in Escherichia coli. Background technique [0002] With the development of recombinant DNA technology and genome sequencing in recent years, a lot of progress has been made in the research and use of gene therapy and DNA vaccines, among which PCR technology has been put into actual medical testing. In the research process, plasmid DNA carrying foreign genes is the main drug carrier used in the research and development of gene therapy and DNA vaccines. The preparation of a large amount of plasmid DNA requires a large number of cultured E. coli to achieve the purpose of preparation. There is a good medium, and the existing medium cannot effectively improve the survival rate and growth rate of E. coli. Contents of the invention [0003] In order to overcome the deficiencies in the prior art, the object of the present in...

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Problems solved by technology

In the research process, plasmid DNA carrying foreign genes is the main drug carrier used in the research and development of gene therapy and DNA vaccines. The preparation of a large amount of plasmid DNA requires a large number of cultured E. coli to achieve the purpose of preparation. There is a good medium, the existing medium can not effectively improve the survival rate and growth rate of E. coli

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